Proteomic analysis of the venom of the predatory ant <i>Pachycondyla striata</i> (Hymenoptera: Formicidae)

Santos, Pollyanna Pereira; Games, Patrícia Dias; Azevedo, Dihego Oliveira; Barros, Edvaldo; Oliveira, Leandro Licursi de; Ramos, Humberto Josué de Oliveira; Baracat‐Pereira, Maria Cristina; Serrão, José Éduardo

doi:10.1002/arch.21424

Cited by 17 publications

(12 citation statements)

References 51 publications

(70 reference statements)

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“…PfA-M1 has various localizations and, in particular, in the food vacuole (a unique proteolytic organelle), where is involved in parasite metabolism and participates in the last steps of hemoglobin degradation [91]. Thus, it is reasonable to assume that BnLCA may participate to the general degradation of host tissues, perhaps increasing their permeability to other venom components and facilitating nutritional exploitation of the host, as suggested for other venom zincmetallopeptidases [92,93].…”

Section: Discussionmentioning

confidence: 99%

Venomics of the ectoparasitoid wasp Bracon nigricans

Becchimanzi¹,

Avolio²,

Bostan³

et al. 2020

BMC Genomics

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Background: Venom is one of the most important sources of regulation factors used by parasitic Hymenoptera to redirect host physiology in favour of the developing offspring. This has stimulated a number of studies, both at functional and "omics" level, which, however, are still quite limited for ectophagous parasitoids that permanently paralyze and suppress their victims (i.e., idiobiont parasitoids). Results: Here we present a combined transcriptomic and proteomic study of the venom of the generalist idiobiont wasp Bracon nigricans, an ectophagous larval parasitoid of different lepidopteran species, for which we recently described the host regulation strategy and the functional role of the venom in the induction of physiological changes in parasitized hosts. The experimental approach used led to the identification of the main components of B. nigricans venom involved in host regulation. Enzymes degrading lipids, proteins and carbohydrates are likely involved in the mobilization of storage nutrients from the fat body and may concurrently be responsible for the release of neurotoxic fatty acids inducing paralysis, and for the modulation of host immune responses. Conclusion:The present work contributes to fill the gap of knowledge on venom composition in ectoparasitoid wasps, and, along with our previous physiological study on this species, provides the foundation on which to develop a functional model of host regulation, based both on physiological and molecular data. This paves the way towards a better understanding of parasitism evolution in the basal lineages of Hymenoptera and to the possible exploitation of venom as source of bioinsecticidal molecules.

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Section: Discussionmentioning

confidence: 99%

Venomics of the ectoparasitoid wasp Bracon nigricans

Becchimanzi¹,

Avolio²,

Bostan³

et al. 2020

BMC Genomics

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“…[ 27,52 ] Additionally, venoms often contain multiple components which can act synergistically or act on different and unrelated targets. [ 3‐5 ] This can be further complicated by variations in the diet and habitat of the insect as well as the season. [ 5,53 ]…”

Section: Discussionmentioning

confidence: 99%

“…[ 3‐5 ] This can be further complicated by variations in the diet and habitat of the insect as well as the season. [ 5,53 ]…”

Section: Discussionmentioning

confidence: 99%

“…[ 2 ] Often venoms contain a mixture of peptides or other biomolecules that act in concert to impair or kill the target. [ 3‐5 ] Nonetheless, when isolated, the individual components can have significant potential as therapeutic agents. [ 6‐9 ] Notably, a number of commercially available drugs such as captopril, eptifibatide, tirofiban, ziconotide, and exenatide all have origins in venoms from a variety of organisms.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the structure‐activity relationship in ponericin L1 from Neoponera goeldii

2020

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Naturally derived antimicrobial peptides have been an area of great interest because of high selectivity against bacterial targets over host cells and the limited development of bacterial resistance to these molecules throughout evolution. There are also a significant number of venom-derived peptides that exhibit antimicrobial activity in addition to activity against mammals or other organisms. Many venom peptides share the same net cationic, amphiphilic nature as host-defense peptides, making them an attractive target for development as potential antibacterial agents. The peptide ponericin L1 derived from Neoponera goeldii was used as a model to investigate the role of cationic residues and net charge on peptide activity. Using a combination of spectroscopic and microbiological approaches, the role of cationic residues and net charge on antibacterial activity, lipid bilayer interactions, and bilayer and membrane permeabilization were investigated. The L1 peptide and derivatives all showed enhanced binding to lipid vesicles containing anionic lipids, but still bound to zwitterionic vesicles. None of the derivatives were especially effective at permeabilizing lipid bilayers in model vesicles, in-tact Escherichia coli, or human red blood cells. Taken together the results indicate that the lack of facial amphiphilicity regarding charge segregation may impact the ability of the L1 peptides to effectively permeabilize bilayers despite effective binding. Additionally, increasing the net charge of the peptide by replacing the lone anionic residue with either Gln or Lys dramatically improved efficacy against several bacterial strains without increasing hemolytic activity. K E Y W O R D S antimicrobial peptides, fluorescence, lipid binding, membrane permeabilization, venom peptides

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“…were able to discover multiple isoforms of crotapotin in the venom of the South American rattlesnake ( Crotalus durissus terrificus ) using de novo sequencing . Further recent studies have applied the technique other organisms, including snails, ants, scorpions, and spiders . Mainly PEAKS and manual de novo sequencing were used to evaluate the MS data in these studies.…”

Section: Practical Applications Of De Novo Sequencing In Proteomicsmentioning

confidence: 99%

A Potential Golden Age to Come—Current Tools, Recent Use Cases, and Future Avenues for De Novo Sequencing in Proteomics

et al. 2018

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In shotgun proteomics, peptide and protein identification is most commonly conducted using database search engines, the method of choice when reference protein sequences are available. Despite its widespread use the database-driven approach is limited, mainly because of its static search space. In contrast, de novo sequencing derives peptide sequence information in an unbiased manner, using only the fragment ion information from the tandem mass spectra. In recent years, with the improvements in MS instrumentation, various new methods have been proposed for de novo sequencing. This review article provides an overview of existing de novo sequencing algorithms and software tools ranging from peptide sequencing to sequence-to-protein mapping. Various use cases are described for which de novo sequencing was successfully applied. Finally, limitations of current methods are highlighted and new directions are discussed for a wider acceptance of de novo sequencing in the community.

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Proteomic analysis of the venom of the predatory ant Pachycondyla striata (Hymenoptera: Formicidae)

Cited by 17 publications

References 51 publications

Venomics of the ectoparasitoid wasp Bracon nigricans

Venomics of the ectoparasitoid wasp Bracon nigricans

Investigation of the structure‐activity relationship in ponericin L1 from Neoponera goeldii

A Potential Golden Age to Come—Current Tools, Recent Use Cases, and Future Avenues for De Novo Sequencing in Proteomics

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