Abstract:Purpose: To investigate global protein expression profiles in the trabecular meshwork (TM) of normal and glucocorticoid-induced ocular hypertensive rat eyes by proteomic analysis, which has not yet been conducted to date. Materials and Methods: A rat ocular hypertension model was produced by topical application of dexamethasone (DEX) for 4 weeks. Age-matched untreated rats served as controls. Intraocular pressure (IOP) was monitored by an electronic tonometer. TM protein expression profiling and protein identi… Show more
“…It is known that glucocorticoids affect extracellular matrix deposition in the trabecular meshwork [6], and alter aqueous proteomics [7] and gene expression. [8,9] There are no such studies on the effect of anti-VEGF agents.…”
Severe and sustained ocular hypertension may occur after intravitreal ranibizumab. Although the mechanism of the pressure rise is unknown, all eyes in our series were controlled with medical therapy.
“…It is known that glucocorticoids affect extracellular matrix deposition in the trabecular meshwork [6], and alter aqueous proteomics [7] and gene expression. [8,9] There are no such studies on the effect of anti-VEGF agents.…”
Severe and sustained ocular hypertension may occur after intravitreal ranibizumab. Although the mechanism of the pressure rise is unknown, all eyes in our series were controlled with medical therapy.
“…Three rats (rats 4, 5, and 6) were treated with topical application of DEX on both eyes for 4 weeks to induce ocular hypertension as described previously. 10,11 The other rats (rats 1, 2, and 3) were treated with vehicle only and served as controls. At the end of treatment, they were used for experiments after IOP measurement conducted with an electronic tonometer as described.…”
Section: Rat Ocular Hypertension Modelmentioning
confidence: 99%
“…At the end of treatment, they were used for experiments after IOP measurement conducted with an electronic tonometer as described. 10,11 All experiments were conducted in compliance with the University of the Ryukyus Animal Care and Use Committee Guidelines and the ARVO Statement for the Use of Animals in Vision Research.…”
Section: Rat Ocular Hypertension Modelmentioning
confidence: 99%
“…10 We used a rat ocular hypertension model generated by relatively short-term topical DEX application, 11 and compared global protein expression profi les in the TM of control and DEX-treated rat eyes. 10 Type I collagen C propeptides, which are processing by-products of fi brillar collagen, were downregulated. The data strongly suggested that collagen turnover, a cycle of homeostatic degradation and repair by newly formed fi brils, is slowed in DEX-treated eyes.…”
Downregulation of CRYAA, SOD1, and TPI1, observed here after a short period of DEX-induced ocular hypertension, may be involved in the onset of neural damage in steroid-induced glaucoma.
“…in uveitic cystoid macula edema [2,4]. However, triamcinolone remained an off-label approach and side effects such as increase in intraocular pressure limit its use [5,6,7]. In addition, because of its short intraocular halftime, reinjections are often needed.…”
Section: Focus On: Intravitreal Therapy In Noninfectious Uveitismentioning
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