Proteomic analysis of rat retina in a steroid-induced ocular hypertension model: Potential vulnerability to oxidative stress

Abstract

Downregulation of CRYAA, SOD1, and TPI1, observed here after a short period of DEX-induced ocular hypertension, may be involved in the onset of neural damage in steroid-induced glaucoma.

Anterior and posterior segment changes in rat eyes with chronic steroid administration and their responsiveness to antiglaucoma drugs

“…In this study, we found significantly increased retinal oxidative stress as indicated by reduced SOD and CAT activities and reduced GSH contents. Global protein expression profiles in the retinae of glucocorticoid treated rats have previously been shown to down-regulate SOD in steroid-induced ocular hypertensive rats by proteomic analysis (Miyara et al, 2008). From these finding it appears likely that in steroid treated eyes, increased retinal oxidative stress as a consequence of elevated IOP might be contributing to loss of GC as observed in retinal morphometry.…”

Intrauterine Growth Restriction Modifies the Developmental Pattern of Intestinal Structure, Transcriptomic Profile, and Bacterial Colonization in Neonatal Pigs

“…These modifications that were evident throughout the entire experimental period for protein and glucose metabolism and from d 2 for lipid metabolism may participate in variations in energy expenditure and nutrient utilization in neonates with IUGR (42). Moreover, the postnatal increase in PTGS2, RPS2, APOA1, SEPP1, and TLR3 and the decrease in VCP expression in ileum (43)(44)(45)(46)(47)(48), and the increase in GPR65 and TIMP1 and decrease in CASP7 expression in the colon (49-51) may be consequences of the increased exposure of fetuses with IUGR to glucocorticoids, as reported (32,33).…”

Retinal Cell Responses to Elevated Intraocular Pressure: A Gene Array Comparison between the Whole Retina and Retinal Ganglion Cell Layer

“…These included Cryab, which in whole retina was found to be downregulated 25-fold, similar to data reported by Ahmed et al 5 Belonging to the small heat shock protein family, ␣-crystallins can serve as molecular chaperones, leading to the speculation that their downregulation could contribute to RGC damage in glaucoma. However, although downregulation of several crystallins has also been reported in the DBA/2J mouse glaucoma model, 7 and reduced ␣A-crystallin was noted by proteomic analysis in a rat glaucoma model, 39 Hsp 27, another small heat shock protein, has been reported to be elevated by several groups in retinas of eyes with experimental glaucoma 40,41 and in human glaucomatous eyes. 42 Other researchers have obtained more variable results, with a suggestion that retinal contamination may play a role.…”