Proteomic Analysis of the Oxidative Stress Response Induced by Low-Dose Hydrogen Peroxide in Bacillus anthracis

Kim, Sang Hoon; Kim, Se Kye; Jung, Kyoung Hwa; Kim, Yun Ki; Hwang, Hyun Chul; Ryu, Sam Gon; Chai, Young Gyu

doi:10.4014/jmb.1209.09070

Cited by 17 publications

(10 citation statements)

References 27 publications

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“…Thus, investigating factors which support the virulence of a pathogen would provide greater insight into the understanding of bacterial physiology for mining new targets for combating infections. Various stresses encountered by the pathogen within the host like iron depletion (Carlson et al, 2009;Cendrowski et al, 2004), oxidative stress (Cybulski et al, 2009;Kim Abbreviations: Pi, inorganic phosphate; PDM, phosphate depleted medium; PBM, phosphate balanced medium; PRM, phosphate repleted medium; BHI, brain heart infusion media; PBS, phosphate buffer saline; MOI, multiplicity of infection; PA, protective antigen; LF, lethal factor; EF, edema factor; DMEM, Dulbecco's modified eagle's medium; MTT, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); ELISA, enzyme linked immunosorbent assay; HRP, horse radish peroxidase; CMS, culture media supernatant. et al, 2013), carbon dioxide stress (McKenzie et al, 2014) etc.…”

Section: Introductionmentioning

confidence: 99%

Phosphate starvation enhances the pathogenesis of Bacillus anthracis

Aggarwal

Somani

Bhatnagar

2015

International Journal of Medical Microbiology

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Section: Introductionmentioning

confidence: 99%

Phosphate starvation enhances the pathogenesis of Bacillus anthracis

Aggarwal

Somani

Bhatnagar

2015

International Journal of Medical Microbiology

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“…In our study, CAT and SOD enzymes showed increased synthesis/activity during UV-B stress, probably to overcome the impacts of UV-B-induced oxidative stress. Over-production of antioxidative enzymes and carotenoid pigments in D. radiodurans and their roles in protecting the ROS-mediated damaging effects have been also reported by different workers [Tian et al, 2007;Kim et al, 2013]. The major goal of this study relates to the identification of differentially formed proteins in D. radiodurans following UV-B exposure.…”

Section: Discussionmentioning

confidence: 72%

Ultraviolet-B Radiation Stress-Induced Toxicity and Alterations in Proteome of Deinococcus radiodurans

Kumar¹,

Ghosh²,

Kumar³

2020

Microb Physiol

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Deinococcus radiodurans is a polyextremophilic bacterium capable to survive and grow at high doses of ionizing radiation. Besides resistance to ionizing radiation, the bacterium is also resistant to toxic chemicals and desiccation. This study deals with the effects of non-ionizing radiation (ultraviolet-B) on survival, alterations in proteomic profile, and gene expression in D. radiodurans. Exposure of culture to UV-B caused decrease in the percentage survival with increasing duration, complete killing occurred after 16 h. D. radiodurans also showed enhancement in the generation of reactive oxygen species and activities of antioxidative enzymes. Separation of proteins by 2-dimensional gel electrophoresis revealed major changes in number and abundance of different proteins. Twenty-eight differentially abundant protein spots were identified by MALDI-TOF MS/MS analysis and divided into 8 groups including unknown proteins. Gene expression of a few identified proteins was also analyzed employing qRT-PCR, which showed differential expression corresponding to the respective proteins. In silico analysis of certain hypothetical proteins (HPs) suggested that these are novel and as yet not reported from D. radiodurans subjected to UV-B stress. These HPs may prove useful in future studies especially for assessing their significance in the adaptation and management of stress responses against UV-B stress.

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“…Recent studies show that the mechanisms of WRF growth inhibition by metals are similar to other microbes (Zeng et al, 2012;Zeng et al, 2015;Zhang et al, 2015). Possible mechanisms of inhibition due to metal-induced toxicity may include; (i) alteration in the morphology of fungal mycelium (Errasquın and Vazquez, 2003;Peña-Castro et al, 2004); (ii) inhibition of intracellular and extracellular proteins due to the denaturation of sulfhydryl groups (Hall, 2002;Kiyono et al, 2010); (iii) disruption in the formation of cell wall by inhibiting Ca +2 dependent regulatory pathways which results in enhanced intercellular Ca +2 efflux (Poirier et al, 2008;Zeng et al, 2012); (iv) induction of oxidative stress due to the inactivation of enzymatic and non-enzymatic antioxidants such as thiols and peroxidases (Kim et al, 2013;Zhang et al, 2015); and (v) formation and accumulation of excessive reactive oxygen species (ROS) namely O −2 , OH −1 and H 2 O 2 , causing severe damage to the cell structure (lipids and proteins) and nucleic acid (DNA and mRNA) as well as enzyme inactivation (Chen et al, 2014;Gupta et al, 2016;Zeng et al, 2012). Despite their potential inhibitory effects, ROS at low concentrations plays an important role in cellular signaling systems such as induction of mutagenic response (Valko et al, 2006;Wan et al, 2015).…”

Section: Growth Inhibition Mechanismsmentioning

confidence: 99%

Impact of wastewater derived dissolved interfering compounds on growth, enzymatic activity and trace organic contaminant removal of white rot fungi – A critical review

Asif

Hai

Hou

et al. 2017

Journal of Environmental Management

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White-rot fungi (WRF) and their ligninolytic enzymes have been investigated for the removal of a broad spectrum of trace organic contaminants (TrOCs) mostly from synthetic wastewater in lab-scale experiments. Only a few studies have reported the efficiency of such systems for the removal of TrOCs from real wastewater. Wastewater derived organic and inorganic compounds can inhibit: (i) WRF growth and their enzyme production capacity; (ii) enzymatic activity of ligninolytic enzymes; and (iii) catalytic efficiency of both WRF and enzymes. It is observed that essential metals such as Cu, Mn and Co at trace concertation (up to 1 mM) can improve the growth of WRF species, whereas non-essential metal such as Pb, Cd and Hg at 1 mM concentration can inhibit WRF growth and their enzyme production. In the case of purified enzymes, most of the tested metals at 1-5 mM concentration do not significantly inhibit the activity of laccases. Organic interfering compounds such as oxalic acid and ethylenediaminetetraacetic acid (EDTA) at 1 mM concentration are potent inhibitors of WRF and their extracellular enzymes. However, inhibitory effects induced by interfering compounds are strongly influenced by the type of WRF species as well as experimental conditions (e.g., incubation time and TrOC type). In this review, mechanisms and factors governing the interactions of interfering compounds with WRF and their ligninolytic enzymes are reviewed and elucidated. In addition, the performance of WRF and their ligninolytic enzymes for the removal of TrOCs from synthetic and real wastewater is critically summarized.

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Proteomic Analysis of the Oxidative Stress Response Induced by Low-Dose Hydrogen Peroxide in Bacillus anthracis

Cited by 17 publications

References 27 publications

Phosphate starvation enhances the pathogenesis of Bacillus anthracis

Phosphate starvation enhances the pathogenesis of Bacillus anthracis

Ultraviolet-B Radiation Stress-Induced Toxicity and Alterations in Proteome of <b><i>Deinococcus radiodurans</i></b>

Impact of wastewater derived dissolved interfering compounds on growth, enzymatic activity and trace organic contaminant removal of white rot fungi – A critical review

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