Proteomic Analysis of the Hyaloid Vascular System Regression during Ocular Development

Abstract

We describe a simple proteomic approach to investigate the differential protein expression patterns and identify the physiologically relevant angiogenic and anti-angiogenic factors involved in the hyaloid vascular system regression. Differentially-expressed proteins were identified using twodimensional gel electrophoresis followed by nano-flow chromatography coupled to tandem mass spectrometry. These proteins are expected to provide insight as to their function in the early maintenance and eventual regression of the hyaloid vascular system.

The capacity of neural crest‐derived stem cells for ocular repair

“…D) and is completed after birth (Stone et al, ). At E16.5, the hyaloid vasculature is sandwiched between the lens and the neural retina (Albe et al, ). Hyaloidal endothelial cells are immunopositive for platelet endothelial cell adhesion molecule (PECAM; Fig.…”

Novel aspects of corneal angiogenic and lymphangiogenic privilege

“…Differentially expressed proteins were identified using two-dimensional gel electrophoresis from the lens and vitreous of P1 and P16 mice (Fig. 18) followed by nanoflow chromatography coupled with tandem mass spectrometry (Albe et al, 2008). Using this approach, the following factors expressed at P16 may be involved in angiogenesis: Tumor necrosis factor-α (TNF-α), hepatoma-derived growth factor (HDGF), fibroblast growth factor-22, and kininogen.…”

“…FGF-22 also induces angiogenesis and stabilizes the endothelial barriers protecting the microvascular and epithelial tissues against mild injuries, and it speeds their repair after major damage (Beyer et al, 2003). Kininogen is a plasma protein that plays important roles in fibrinolysis, thrombosis, and inflammation (Albe et al, 2008). …”

“…Representative 2-DE gels of proteins obtained from the lens and vitreous of P1 mouse and P16 mouse using IPG strips with pH range 4–7 and 7–10. The proteins excised for analysis and identification by MS are marked with numbers from 1 to 46 (reprinted with permission from Albe et al, 2008). …”

Semaphorin 7A Promotes Angiogenesis in an Experimental Corneal Neovascularization Model

“…Recent studies have reported that semaphorins, including Semaphorin 3B, 3F, 3E, 4D, and 6A, can either promote or inhibit tumor angiogenesis and progression 4–7 . We previously detected Semaphorin 7A (Sema7a) expression in mouse ocular tissues during the course of ocular angiogenesis analysis 8, 9 .…”