Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t

Tian, Dagang; Liu, Yang; Chen, Zaijie; Chen, Ziqiang; Wang, Feng; Zhou, Yuanchang; Luo, Yuming; Yang, Liming; Chen, Songbiao

doi:10.1186/s12284-018-0240-3

Cited by 16 publications

(19 citation statements)

References 30 publications

(44 reference statements)

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“…2). Similar to this present investigation, comparative proteome evaluation was also carried out with a single transgenic plant line in rice 29–31 , soybean 32 , and maize 33 . Although the leaf morphology was not largely affected post 24 and 48 h of infection, the molecular signalling network was speculated to be altered for generating a novel leaf proteome.…”

Section: Resultsmentioning

confidence: 92%

Proteo-metabolomic investigation of transgenic rice unravels metabolic alterations and accumulation of novel proteins potentially involved in defence against Rhizoctonia solani

Karmakar

Datta

Molla

et al. 2019

Sci Rep

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The generation of sheath blight (ShB)-resistant transgenic rice plants through the expression of Arabidopsis NPR1 gene is a significant development for research in the field of biotic stress. However, to our knowledge, regulation of the proteomic and metabolic networks in the ShB-resistant transgenic rice plants has not been studied. In the present investigation, the relative proteome and metabolome profiles of the non–transformed wild-type and the AtNPR1- transgenic rice lines prior to and subsequent to the R. solani infection were investigated. Total proteins from wild type and transgenic plants were investigated using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (MS). The metabolomics study indicated an increased abundance of various metabolites, which draws parallels with the proteomic analysis. Furthermore, the proteome data was cross-examined using network analysis which identified modules that were rich in known as well as novel immunity-related prognostic proteins, particularly the mitogen-activated protein kinase 6, probable protein phosphatase 2C1, probable trehalose-phosphate phosphatase 2 and heat shock protein. A novel protein, 14–3–3GF14f was observed to be upregulated in the leaves of the transgenic rice plants after ShB infection, and the possible mechanistic role of this protein in ShB resistance may be investigated further.

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Section: Resultsmentioning

confidence: 92%

Proteo-metabolomic investigation of transgenic rice unravels metabolic alterations and accumulation of novel proteins potentially involved in defence against Rhizoctonia solani

Karmakar

Datta

Molla

et al. 2019

Sci Rep

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“…In our earlier studies, four proteins of a receptor-like protein kinase (gi|59,800,021), a NADP dependent malic enzyme (gi|54,606,800), a putative transaldolase (gi|57, 900,129), and a putative bowman birk trypsin inhibitor (gi|53,792,234), three C3HC4-type Ring finger E3 ubiquitin ligase (APIP2, 6, 10), and a bowman-Birk type bran trypsin inhibitor (APIP4) were confirmed to be involved in Piz-t-KJ201 interaction (Park et al 2012(Park et al , 2016Tian et al 2018;Zhang et al 2020). In the present study, we observed that a receptor-like protein kinase precursor (LOC_Os10g33040), a NADP-dependent malic enzyme (LOC_Os05g09440), a transaldolase (LOC_ Os08g05830), a bowman-Birk type bran trypsin inhibitor precursor (LOC_Os01g03360), and two zinc finger C3HC4 type domain containing protein (LOC_ Os01g20910 and LOC_Os12g04590) were also differentially regulated in Piz-t-KJ201 (Additional Table 2).…”

Section: Validation Of the Present Study By Comparing Previous Reportsmentioning

confidence: 99%

“…This gene was once widely used in breeding programs for increasing resistance to M. oryzae (Zhou et al 2006;Tian et al 2020). Numerous studies also have examined the interaction profiles of Piz-t-AvrPiz-t in rice (Park et al 2012(Park et al , 2016Wang et al 2016;Tang et al 2017;Tian et al 2018;Bai et al 2019;Zhang et al 2020). However, transcriptome profiling underlying the Piz-t-mediated rice resistance to blast fungus remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Weighted Gene Co-Expression Network Coupled with a Critical-Time-Point Analysis during Pathogenesis for Predicting the Molecular Mechanism Underlying Blast Resistance in Rice

Tian

Chen

Lin

et al. 2020

Rice

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Background Rice blast, caused by the ascomycete fungus M. oryzae, is one of the most important diseases of rice. Although many blast resistance (R) genes have been identified and deployed in rice varieties, the molecular mechanisms responsible for the R gene-mediated defense responses are yet not fully understood. Results In this study, we used comparative transcriptomic analysis to explore the molecular mechanism involved in Piz-t-mediated resistance in a transgenic line containing Piz-t (NPB-Piz-t) compared to Nipponbare (NPB). Clustering and principal component analysis (PCA) revealed that the time-point at 24-h post inoculation (hpi) was the most important factor distinguishing the four time-points, which consisted of four genes of mitogen-activated protein kinases (MAPKs) signaling pathway, one gene related to WRKY DNA-binding domain containing protein, five pathogenesis-related protein (OsPR1s) genes, and three genes of R proteins involving in the most significant protein-protein interaction (PPI) pathway. Using weighted gene co-expression network analysis (WGCNA) to investigate RNA-seq data across 0, 24, 48, and 72 hpi, nine modules with similar patterns expression pattern (SEP) and three modules with differential expression pattern (DEP) between NPB-Piz-t and NPB across 0, 24, 48, and 72 hpi with KJ201 (referred to as Piz-t-KJ201 and NPB-KJ201) were identified. Among these the most representative SEP green-yellow module is associated with photosynthesis, and DEP pink module comprised of two specific expressed nucleotide-binding domain and leucine-rich repeat (NLR) genes of LOC_Os06g17900 and LOC_Os06g17920 of Pi2/9 homologous, three NLR genes of LOC_Os11g11810, LOC_Os11g11770, and LOC_Os11g11920 which are putatively associated with important agronomic traits, and a B3 DNA binding domain containing protein related genes (LOC_Os10g39190). Knockout of LOC_Os10g39190 via CRISPR-Cas9 resulted in plant death at the seedling stage. Conclusions The research suggested that Piz-t and multiple NLR network might play important roles in the regulation of the resistance response in the Piz-t-KJ201 interaction system. The identified genes provide an NLR repository to study the rice-M. oryzae interaction system and facilitate the breeding of blast-resistant cultivars in the future.

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“…Moreover, an RLK was observed to be induced by M. oryzae inoculation in the NPB and NPB-Piz-t rice lines. In addition to PRs and RLKs, a putative bowman birk trypsin inhibitor, which might be involved in AvrPiz-t/Piz-t networks, was induced by M. oryzae infection in both compatible and incompatible interactions (Tian et al, 2018).…”

Section: Key Proteome Responses Underlying Rice Resistance To M Oryzaementioning

confidence: 99%

Proteomics of Rice—Magnaporthe oryzae Interaction: What Have We Learned So Far?

et al. 2019

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Rice blast disease, caused by Magnaporthe oryzae, is one of the major constraints to rice production, which feeds half of the world’s population. Proteomic technologies have been used as effective tools in plant−pathogen interactions to study the biological pathways involved in pathogen infection, plant response, and disease progression. Advancements in mass spectrometry (MS) and apoplastic and plasma membrane protein isolation methods facilitated the identification and quantification of subcellular proteomes during plant-pathogen interaction. Proteomic studies conducted during rice−M. oryzae interaction have led to the identification of several proteins eminently involved in pathogen perception, signal transduction, and the adjustment of metabolism to prevent plant disease. Some of these proteins include receptor-like kinases (RLKs), mitogen-activated protein kinases (MAPKs), and proteins related to reactive oxygen species (ROS) signaling and scavenging, hormone signaling, photosynthesis, secondary metabolism, protein degradation, and other defense responses. Moreover, post−translational modifications (PTMs), such as phosphoproteomics and ubiquitin proteomics, during rice−M. oryzae interaction are also summarized in this review. In essence, proteomic studies carried out to date delineated the molecular mechanisms underlying rice-M. oryzae interactions and provided candidate proteins for the breeding of rice blast resistant cultivars.

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Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t

Cited by 16 publications

References 30 publications

Proteo-metabolomic investigation of transgenic rice unravels metabolic alterations and accumulation of novel proteins potentially involved in defence against Rhizoctonia solani

Proteo-metabolomic investigation of transgenic rice unravels metabolic alterations and accumulation of novel proteins potentially involved in defence against Rhizoctonia solani

Weighted Gene Co-Expression Network Coupled with a Critical-Time-Point Analysis during Pathogenesis for Predicting the Molecular Mechanism Underlying Blast Resistance in Rice

Proteomics of Rice—Magnaporthe oryzae Interaction: What Have We Learned So Far?

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