Proteomic analysis of the compatible interaction of wheat and powdery mildew ( Blumeria graminis f. sp. tritici )

Li, Jie; Yang, Xin; Liu, Xinhao; Yu, Haibo; Du, Congyang; Li, Mengda; He, Dexian

doi:10.1016/j.plaphy.2016.12.006

Cited by 21 publications

(23 citation statements)

References 43 publications

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“…The up-regulation of these genes suggested that the photosynthetic apparatus was not undergoing degradation after 48 h of infection and hence not enough to lead to a reduction in the photosynthetic rate, maybe associated with that the growth stage of pathogens, which coincides with the appearance of primary hyphae ( Fig 1C ). These results in this study were not in agreement with the statement of a previous report, according to which the expression profiles of many proteins involved in photosynthesis were mostly down-regulated in PM infected wheat leaves [ 38 ]. Interestingly, two DEGs encoding the chloroplastic ATP synthesis-related protein (c47457_g1 and c115085_g1) were up-regulated both at 24 hpi and 48 hpi.…”

Section: Resultscontrasting

confidence: 99%

Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew

Guo

Chen

et al. 2018

PLoS ONE

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Cucurbit powdery mildew (PM) is one of the most severe fungal diseases, but the molecular mechanisms underlying PM resistance remain largely unknown, especially in pumpkin (Cucurbita moschata Duch.). The goal of this study was to identify gene expression differences in PM-treated plants (harvested at 24 h and 48 h after inoculation) and untreated (control) plants of inbred line “112–2” using RNA sequencing (RNA-Seq). The inbred line “112–2” has been purified over 8 consecutive generations of self-pollination and shows high resistance to PM. More than 7600 transcripts were examined in pumpkin leaves, and 3129 and 3080 differentially expressed genes (DEGs) were identified in inbred line “112–2” at 24 and 48 hours post inoculation (hpi), respectively. Based on the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway database and GO (Gene Ontology) database, a complex regulatory network for PM resistance that may involve hormone signal transduction pathways, transcription factors and defense responses was revealed at the transcription level. In addition, the expression profiles of 16 selected genes were analyzed using quantitative RT-PCR. Among these genes, the transcript levels of 6 DEGs, including bHLH87 (Basic Helix-loop-helix transcription factor), ERF014 (Ethylene response factor), WRKY21 (WRKY domain), HSF (heat stress transcription factor A), MLO3 (Mildew Locus O), and SGT1 (Suppressor of G-Two Allele of Skp1), in PM-resistant “112–2” were found to be significantly up- or down-regulated both before 9 hpi and at 24 hpi or 48 hpi; this behavior differed from that observed in the PM-susceptible material (cultivar “Jiujiangjiaoding”). The transcriptome data provide novel insights into the response of Cucurbita moschata to PM stress and are expected to be highly useful for dissecting PM defense mechanisms in this major vegetable and for improving pumpkin breeding with enhanced resistance to PM.

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Section: Resultscontrasting

confidence: 99%

Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew

Guo

Chen

et al. 2018

PLoS ONE

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“…Thus, the accumulation of Pro in mildew-infected wheat indicated that the wheat defensive mechanisms had been triggered. Based on the qPCR and enzyme activity data shown in Figures 4 , 5 , we found that mildew infection significantly induced defense responses in wheat, which was consistent with the findings of previous proteomic and transcriptomic investigations on the effects of mildew infection of wheat ( Xin et al, 2012 ; Tayeh et al, 2015 ; Fu et al, 2016 ; Li et al, 2017 ). In these studies, numerous genes involved in phytohormone metabolism and defensive signaling pathways were upregulated in response to mildew infection, including SA, abscisic acid, gibberellic acid, ET, Auxin, and cytokinin.…”

Section: Discussionsupporting

confidence: 90%

Infection of Powdery Mildew Reduces the Fitness of Grain Aphids (Sitobion avenae) Through Restricted Nutrition and Induced Defense Response in Wheat

et al. 2018

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In natural ecological systems, plants are often simultaneously attacked by both insects and pathogens, which can affect each other’s performance and the interactions can be extended to higher trophic levels, such as parasitoids. The English grain aphid (Sitobion avenae) and powdery mildew (Blumeria graminis f. sp. tritici) are two common antagonists that pose a serious threat to wheat production. Numerous studies have investigated the effect of a single factor (insect or pathogen) on wheat production. However, investigation on the interactions among insect pests, pathogens, and parasitoids within the wheat crop system are rare. Furthermore, the influence of the fungicide, propiconazole, has been found to imitate the natural ecosystem. Therefore, this study investigated the effects of B. graminis on the biological performance of grain aphids and the orientation behavior of its endoparasitic wasp Aphidius gifuensis in the wheat system. Our findings indicated that B. graminis infection suppressed the feeding behavior, adult and nymph weight, and fecundity and prolonged the developmental time of S. avenae. We found that wheat host plants had decreased proportions of essential amino acids and higher content of sucrose following aggravated B. graminis infection. The contents of Pro and Gln increased in the wheat plant tissues after B. graminis infection. In addition, B. graminis infection elicited immune responses in wheat: increase in the expression of defense genes, content of total phenolic compounds, and activity of three related antioxidant enzymes. Moreover, co-infection of B. graminis and S. avenae increased the attraction to A. gifuensis compare to that after infestation with aphids alone. In conclusion, our results indicated that B. graminis infection adversely affected the performance of S. avenae in wheat through restricted nutrition and induced defense response. Furthermore, the preference of parasitoids in such an interactive environment might provide an important basis for pest management control.

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“…4b ). Interestingly, the proteome data of Li et al 41 indicated that the expression of MAPK5 is downregulated in susceptible wheat leaves after Blumeria graminis f. sp. tritic infection.…”

Section: Discussionmentioning

confidence: 99%

“…4b ). Among these genes, 6-phosphogluconate dehydrogenase , which encodes a protein positively induced by powdery mildew in wheat leaves, has been reported to activate host defences 41 , and three UDP-glycosyltransferase genes that are closely related to the mechanism of galloylated catechins and flavonol 3- O -guycosides in tea were induced 55 . We previously demonstrated that the content of (-)-epigallocatechin-3-gallate and caffeine rapidly accumulated after C. fructicola infection; at the same time, the expression levels of key genes associated with flavonoids and the caffeine metabolism pathway were clearly upregulated, including the phenylalanine ammonia-lyase ( PAL ) and S -adenosylmethionine synthetase ( SAMS ) 4 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis and histochemistry reveal that hypersensitive cell death and H2O2 have crucial roles in the resistance of tea plant (Camellia sinensis (L.) O. Kuntze) to anthracnose

Wang

Hao

et al. 2018

Hortic Res

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Anthracnose causes severe losses of tea production in China. Although genes and biological processes involved in anthracnose resistance have been reported in other plants, the molecular response to anthracnose in tea plant is unknown. We used the susceptible tea cultivar Longjing 43 and the resistant cultivar Zhongcha 108 as materials and compared transcriptome changes in the leaves of both cultivars following Colletotrichum fructicola inoculation. In all, 9015 and 8624 genes were differentially expressed between the resistant and susceptible cultivars and their controls (0 h), respectively. In both cultivars, the differentially expressed genes (DEGs) were enriched in 215 pathways, including responses to sugar metabolism, phytohormones, reactive oxygen species (ROS), biotic stimuli and signalling, transmembrane transporter activity, protease activity and signalling receptor activity, but DEG expression levels were higher in Zhongcha 108 than in Longjing 43. Moreover, functional enrichment analysis of the DEGs showed that hydrogen peroxide (H2O2) metabolism, cell death, secondary metabolism, and carbohydrate metabolism are involved in the defence of Zhongcha 108, and 88 key genes were identified. Protein–protein interaction (PPI) network demonstrated that putative mitogen-activated protein kinase (MAPK) cascades are activated by resistance (R) genes and mediate downstream defence responses. Histochemical analysis subsequently validated the strong hypersensitive response (HR) and H2O2 accumulation that occurred around the hyphal infection sites in Zhongcha 108. Overall, our results indicate that the HR and H2O2 are critical mechanisms in tea plant defence against anthracnose and may be activated by R genes via MAPK cascades.

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Proteomic analysis of the compatible interaction of wheat and powdery mildew ( Blumeria graminis f. sp. tritici )

Cited by 21 publications

References 43 publications

Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew

Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew

Infection of Powdery Mildew Reduces the Fitness of Grain Aphids (Sitobion avenae) Through Restricted Nutrition and Induced Defense Response in Wheat

Transcriptional analysis and histochemistry reveal that hypersensitive cell death and H2O2 have crucial roles in the resistance of tea plant (Camellia sinensis (L.) O. Kuntze) to anthracnose

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