Proteomic analysis of the abomasal mucosal response following infection by the nematode, Haemonchus contortus, in genetically resistant and susceptible sheep

Transcriptional profiling of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance

“…We examined gene expression in a breed that primarily manifests resistance by regulating worm fecundity, although regulation of worm burden may also contribute (McRae et al, 2014;Stear et al, 1995;Stear et al, 1996b). Despite the differences between the present study and previous studies, many of the DE genes observed in this study were previously reported in other studies examining resistance to GIN in sheep via regulation of worm burden, including those investigating the transcriptome (Ahmed, 2013;Gossner et al, 2013) and the proteome (Nagaraj et al, 2012). Only 1 DE gene (IL13) was common with Salleí et al (2014), a study which examined gene expression differences in lambs that controlled worm fecundity, although this may be due to the fact that they examined a limited number of genes, at a different time-point, and in lambs infected with a different parasite species .…”

“…ALB has been reported to be more highly expressed in the duodenum of helminth-naïve genetically-resistant animals compared to susceptible individuals (Keane et al, 2006) and to decline in the abomasum of sheep during the course of repeated truncated immunising infections with Trichostrongylus colubriformis larvae (Knight et al, 2010). ALB levels were also increased in the abomasal mucosa of resistant selectionline animals compared to their susceptible counterparts 3 days after experimental challenge with Haemonchus contortus (Nagaraj et al, 2012). In a separate study on changes in abomasal protein expression following trickle infection with T. circumcincta, ALB was relatively highly expressed in the mucosa of helminth-naïve animals in comparison to both their immune and immune-waning counterparts (Pemberton et al, 2012).…”

“…Expression of MX2 is strongly induced by IFN-␣ (Kane et al, 2013;Melén et al, 1996), and the MX2 protein shows antiviral activity (Sasaki et al, 2014). Despite the association between this gene and viral infections, it has previously been shown to be increased at both the mRNA and protein levels in GIN susceptible animals (Ahmed, 2013;Nagaraj et al, 2012). The genes LYVE1 (Lymphatic vessel endothelial hyaluronan receptor 1) and CHI3L2 (chitinase 3-like 2) were more highly expressed in HighFEC animals at 14 compared to 7 dpi.…”

Gastrokines: stomach-specific proteins with putative homeostatic and tumor suppressor roles

“…We observed Gkn3 to be upregulated in murine gastric atrophy and mucous cell metaplasia (42), which are precancerous lesions known typically to be associated with H. pylori infection in mice and humans (17). Upregulation of GKN3 expression was also seen following nematode infection in genetically susceptible sheep compared with resistant sheep (46). In summary, animal models have been instrumental in revealing differential responses of individual GKNs to gastric disease.…”

Proteomic Analysis Revealed the Important Role of Vimentin in Human Cervical Carcinoma HeLa Cells Treated With Gambogic Acid

“…The phosphorylation state of vimentin was checked using nanoHPLC-ESI MS/MS after enrichment of phosphopeptides. Phosphopeptides were enriched using TiO2 (17) and the nanoHPLC-ESI MS/MS analysis was performed with a TripleTOF 5600 system (AB Sciex) as reported before (18). Briefly, 1 mg protein sample was reduced with 100 mM ammonium bicarbonate and 100 mM dithiothreitol in a reaction volume of 250 l by heating at 56°C for 60 min.…”