Transcriptional profiling of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance

McRae, Kathryn M.; Good, Barbara; Hanrahan, J. P.; McCabe, Matthew S.; Cormican, Paul; Sweeney, T.; O’Connell, Mary J.; Keane, Orla M.

doi:10.1016/j.vetpar.2016.05.014

Cited by 22 publications

(23 citation statements)

References 59 publications

(80 reference statements)

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“…One major problem for goats and sheep genomic analysis was that the functional analysis was not available with goats or sheep as reference species due to the lack of data for C. hircus and Ovis aries in functional analysis programs. Therefore, almost all previous publications in goats and sheep genomic filled used human [27][28][29] or bovine [30] genome as reference for functional analysis.…”

Section: Discussionmentioning

confidence: 99%

Genomic variants from RNA-seq for goats resistant or susceptible to gastrointestinal nematode infection

Aboshady¹,

Mandonnet²,

Johansson³

et al. 2019

Preprint

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Background Gastrointestinal nematodes (GIN) are an important constraint in grazing small ruminant production. Genetic selection for resistant animals is a potential sustainable control strategy. Advances in molecular genetics have led to the identification of several molecular genetic markers associated with genes affecting economic relevant traits.Results In this study, the variants in the genome of Creole goats resistant or susceptible to GIN were discovered from RNA-sequencing. We identified SNPs, insertions and deletions that distinguish the two genotypes, resistant and susceptible and we characterized these variants through functional analysis. The T cell receptor signalling pathway was one of the top significant pathways that distinguish the resistant from the susceptible genotype with 78% of the genes involved in this pathway showing genomic variants.Conclusions These genomic variants are expected to provide useful resources especially for molecular breeding for GIN resistance in goats.

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Section: Discussionmentioning

confidence: 99%

Genomic variants from RNA-seq for goats resistant or susceptible to gastrointestinal nematode infection

Aboshady¹,

Mandonnet²,

Johansson³

et al. 2019

Preprint

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“…The expression of Gal - 14 was maximum at day 10 post-challenge in yearling Scottish Blackface lambs previously infected with T. circumcincta [ 9 ]. In Scottish Blackface lambs, the differential expression of the Gal - 14 gene was reported in abomasal lymph node of resistant animals after 14-day post-infection with T. circumcincta [ 14 ] (Table 2 ). In addition, a role of galectins has been widely reported in reference to different host parasite interactions and these proteins appear to be responsible for adhesion of pathogens to host cells and host adaptive immunity [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…Also, the expression of other SLC family members were reported in response to nematode infection in both susceptible and resistant sheep and variants in the SLC22A4 gene have been associated to Inflammatory Bowel disease in humans [ 53 ]. Whereas, high expression of SLC30A2 in susceptible sheep in response to T. circumcincta infection has been reported in Scottish Blackface lambs [ 14 ], H. contortus infection shows a significant impact on the expression of the SLC2A3 gene in Canary sheep breeds [ 15 ] (Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…As an alternative method to identify genes linked to GIN resistance, studies based on gene expression analysis can identify those genes whose expression may differ between animals and the gene pathways activated depending on the status against infection. In sheep infected by GIN, these kinds of studies based on expression analysis by RT-PCR of specific candidate genes [ 9 – 11 ], the use of microarrays [ 12 , 13 ] or recently on the RNA-Seq technology [ 14 , 15 ], have been mainly focused on young animals. For example, for lambs infected with GIN, different studies have shown that the animal status is dependent on the different type of host immune response that is activated against the nematodes in the abomasal (gastric) mucosa and abomasal lymph node [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes

Chitneedi

Suárez-Vega

Martínez‐Valladares

et al. 2018

Vet Res

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The present study exploited the RNA-seq technology to analyze the transcriptome of target tissues affected by the Teladorsagia circumcincta infection in two groups of adult ewes showing different statuses against gastrointestinal nematode (GIN) infection with the aim of identifying genes linked to GIN infection resistance in sheep. For this, based on the accumulated faecal egg count of 18 adult Churra ewes subjected to a first experimental infection with T. circumcincta, six ewes were classified as resistant and six others as susceptible to the infection. These 12 animals were dewormed and infected again. After humanitarian sacrifice of these 12 animals at day 7 post-infection, RNA samples were obtained from abomasal mucosa and lymph node tissues and RNA-Seq datasets were generated using an Illumina HiSeq 2000 sequencer. The distribution of the genes based on their expression level were very similar among the two different tissues and conditions. The differential expression analysis performed with two software (DESeq and EdgeR) only identified common differentially expressed genes (DEGs), a total of 106, in the lymph node samples which were considered as GIN-activated. The enrichment analysis performed for these GIN-activated genes identified some pathways related to cytokine-mediated immune response and the PPARG signaling pathway as well as disease terms related to inflammation and gastro-intestinal diseases as enriched. A systematic comparison with the results of previous studies confirmed the involvement of genes such as ITLN2, CLAC1 and galectins, in the immune mechanism activated against T. circumcincta in resistant sheep.Electronic supplementary materialThe online version of this article (10.1186/s13567-018-0534-x) contains supplementary material, which is available to authorized users.

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“…Scottish Blackface lambs ( n = 258) were sourced over 2 years (2010, 2011) from the flock at the Teagasc Hill Sheep Farm, Leenane, Co. Mayo, and individual animal samples were collected as described previously (McRae et al . , ). FEC was determined using the modified McMaster method (Ministry of Agriculture, Fish & Food ), and egg counts were distinguished as Nematodirus spp.…”

Section: Details Of Genomic Regions Validated In Sheep Populations Inmentioning

confidence: 99%

An independent validation study of loci associated with nematode resistance in sheep

et al. 2018

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Gastrointestinal nematode infection is a constraint on sheep production worldwide. Selective breeding programmes to enhance resistance to nematode infection are currently being implemented in a number of countries. Identification of loci associated with resistance to infection or causative mutations for resistance would enable more effective selection. Loci associated with indicator traits for nematode resistance has been identified in previous studies. In this study, Scottish Blackface, Texel and Suffolk lambs were used to validate the effects at eight genomic regions previously associated with nematode resistance (OAR3, 4, 5, 7, 12, 13, 14, 21). No SNP was significantly associated with nematode resistance at the region-wide level but seven SNPs in three of the regions (OAR4, 12, 14) were nominally associated with trichostrongyle egg count in this study and six of these were also significant when fitted as single SNP effects. Nematodirus egg count was nominally associated with SNPs on OAR3, 4, 7 and 12.

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Transcriptional profiling of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance

Abstract: of the ovine abomasal lymph node reveals a role for timing of the immune response in gastrointestinal nematode resistance.Veterinary Parasitology http://dx

Cited by 22 publications

References 59 publications

Genomic variants from RNA-seq for goats resistant or susceptible to gastrointestinal nematode infection

Genomic variants from RNA-seq for goats resistant or susceptible to gastrointestinal nematode infection

Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes

An independent validation study of loci associated with nematode resistance in sheep

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