Proteomic Analysis of Specific Proteins in the Root of Salt-Tolerant Barley

Abstract

Comparative two-dimensional electrophoresis showed six proteins, which were significantly produced in the root of salt-tolerant barley. These proteins were identified as stress/defense-related proteins that do not scavenge reactive oxygen species directly, suggesting that salt-tolerant barley develops not only an antioxidative system, but also physical and biochemical changes to cope with salt stress.

Effect of salinity stress on plants and its tolerance strategies: a review

“…With the consistent alterations in energy metabolism, there is an increased risk of oxidative damage in salt-treated plants that leads to the formation of ROS and enhanced ROS formation results in an increased relative abundance of several ROS scavenging enzymes such as catalase (CAT), superoxide dismutase (SOD), enzymes of ascorbate-glutathione cycle: ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR; Kav et al 2004;Sugimoto and Takeda 2009), cytochrome P450 monooxygenase, thioredoxin h (Trx h), glutathione-S-transferase (GST), etc., and other proteins involved in maintenance of plant redox status-protein disulfide isomerase (Kim et al 2005;Dooki et al 2006;Aghaei et al 2008;Wang et al 2008a;Geissler et al 2010;Pang et al 2010;Kumar Swami et al 2011). Besides activation of ROS scavenging enzymes, other responses could be observed in salttreated plants.…”

Comparative proteomic analysis of salt response proteins in seedling roots of two wheat varieties

“…A transgenic Brassica napus plant constitutively expressed a pea PR10 gene that exhibited enhanced tolerance to salt stress [50]. Considerable work revealed that PR protein expression was increased significantly in salinity-stressed plants such as saline-tolerant peanut and salt-tolerant barley roots [51,52]. It can be concluded that up-regulation of PR10 contributed to the higher salt-tolerance of Jing-411 compared to CS, especially at lower salt concentrations.…”

MS based proteomic approaches for analysis of barley malt

“…Numbers of articles dealing with plant proteomics found by the search engine "Web of Sciences" using the key words: (plant* or cereal* or vegetable* or fruit*) and (proteomic* or protein*); years 2000e2011. Kaspar et al, 2010), root (Sugimoto, and Takeda, 2009) and shoots (Süle et al, 2004). However, the majority of the studies of barley are on grains or their individual parts: the aleurone layer, starchy endosperm and embryo (Finnie and Svensson, 2003).…”