Proteomic analysis of somatic embryogenesis in Vitis vinifera

Abstract

Two dimensional gel electrophoresis coupled to mass spectrometry has been used to study the somatic embryogenesis in Vitis vinifera, by comparing embryogenic and non embryogenic calluses of the Thompson seedless cv. More than 1,000 spots were reproducibly resolved in colloidal Coomassie brilliant blue stained gels over a pI nonlinear range of 3-10 in the first dimension and using homogeneous 12.5% polyacrylamide gels in the second dimension. The expression pattern of 35 spots differed significantly between the two samples. These spots were processed by mass spectrometry analysis and the protein identity was assigned by using both the non-redundant protein and EST databases. Several responsive proteins, some already known to be involved in the somatic embryogenesis process while others, for the first time put into relation with this process, have been described. Moreover, they have been subdivided in functional categories, and their putative role is discussed in terms of their relevance in the somatic embryogenesis process.

Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species

“…The characterization of profiles and changes in protein content during seed development allows for comparing in vitro and in vivo embryo development. However, although several embryogenically expressed proteins have been identified so far, there have been few reports focused on woody plants (Marsoni et al 2008), even less so regarding proteomic characterization throughout recalcitrant seed development (Pawlowski et al 2007;Silveira et al 2008;Balbuena et al 2009). …”

Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains

“…Total proteins (500 mg) were loaded onto an 18-cm, pH 4-7 linear gradient or pH 3-10 nonlinear gradient (NL) Immobiline DryStrip gels (IPG strips) (GE Healthcare, Uppsala, Sweden), and separated as described by Marsoni et al (2008). The SDS-PAGE gels were visualised by the modified Colloidal Coomassie Brilliant Blue staining method (Aina et al 2007).…”

“…Spot digestion was performed as previously described (Marsoni et al 2008). The extracted tryptic fragments were analysed by MS/ MS after reverse-phase separation of peptides by liquid chromatography-electrospray ionisation MS/MS (LC-ESI-MS/ MS).…”

Estabelecimento de marcadores bioquímicos para embriogênese somática em Araucaria angustifolia.

“…persicum (Lyngved et al, 2008;Winkelmann et al, 2006), V. vinifera (Marsoni et al, 2008) e Citrus sinensis (Pan et al, 2009). Em A. angustifolia, esta abordagem foi estabelecida e empregada, sendo identificadas uma série de proteínas marcadoras de estádio específicos durante os processos do desenvolvimento do embrião zigótico e germinação da semente (Balbuena et al, 2009dos Santos et al, 2006;Silveira et al, 2008;).…”