Proteomic Analysis of Serum Cytokine Levels in Response to Highly Active Antiretroviral Therapy (HAART)

Relucio, Karen I.; Beernink, Hans; Chen, Donna; Israelski, Dennis; Kim, Woojin; Holodniy, Mark

doi:10.1021/pr049930y

Cited by 17 publications

(11 citation statements)

References 30 publications

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“…Analytes found to be elevated in chronic infection in this study have been shown to be elevated using separate test methodologies for TNF-α [19–21] and IP-10 [34]. During primary infection IP-10 and TNF-α correlated positively with quantitative viral load [35].…”

Section: Discussionmentioning

confidence: 95%

The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women

Keating

Golub

Nowicki

et al. 2011

Aids

119

100

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Objective HIV causes inflammation that can be at least partially corrected by HAART. To determine the qualitative and quantitative nature of cytokine perturbation, we compared cytokine patterns in three HIV clinical groups including HAART responders (HAART), untreated HIV non-controllers (NC), and HIV-uninfected (NEG). Methods Multiplex assays were used to measure 32 cytokines in a cross-sectional study of participants in the Women's Interagency HIV Study (WIHS). Participants from 3 groups were included: HAART (n=17), NC (n=14), and HIV NEG (n=17). Results Several cytokines and chemokines showed significant differences between NC and NEG participants, including elevated IP-10 and TNF-α and decreased IL-12(p40), IL-15, and FGF-2 in NC participants. Biomarker levels among HAART women more closely resembled the NEG, with the exception of TNF-α and FGF-2. Secondary analyses of the combined HAART and NC groups revealed that IP-10 showed a strong, positive correlation with viral load and negative correlation with CD4+ T cell counts. The growth factors VEGF, EGF, and FGF-2 all showed a positive correlation with increased CD4+ T cell counts. Conclusion Untreated, progressive HIV infection was associated with decreased serum levels of cytokines important in T cell homeostasis (IL-15) and T cell phenotype determination (IL-12), and increased levels of innate inflammatory mediators such as IP-10 and TNF-α. HAART was associated with cytokine profiles that more closely resembled those of HIV uninfected women. The distinctive pattern of cytokine levels in the 3 study groups may provide insights into HIV pathogenesis, and responses to therapy.

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Section: Discussionmentioning

confidence: 95%

The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women

Keating

Golub

Nowicki

et al. 2011

Aids

119

100

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“…These arrays have the capacity to measure a few cytokines to more than 30 in a sample. Using protein microarrays, cytokines have been detected from cell culture [58], wound fluids [59], dermal [60, 61] and pancreatic fluid [61] and to determine the effectiveness of antiviral therapies [62]. Zajac et al, described a protein microarray for measurement of IL-1β, IL-6, IL-8, IL-13, MIP-1β, and TNF-α using quantum dots [63].…”

Section: Standard Cytokine Assaysmentioning

confidence: 99%

Bioanalytical chemistry of cytokines – A review

Stenken

Poschenrieder

2015

Analytica Chimica Acta

233

172

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Cytokines are bioactive proteins produced by many different cells of the immune system. Due to their role in different inflammatory disease states and maintaining homeostasis, there is enormous clinical interest in the quantitation of cytokines. The typical standard methods for quantitation of cytokines are immunoassay-based techniques including enzyme-linked immusorbent assays (ELISA) and bead-based immunoassays read by either standard or modified flow cytometers. A review of recent developments in analytical methods for measurements of cytokine proteins is provided. This review briefly covers cytokine biology and the analysis challenges associated with measurement of these biomarker proteins for understanding both health and disease. New techniques applied to immunoassay-based assays are presented along with the uses of aptamers, electrochemistry, mass spectrometry, optical resonator-based methods. Methods used for elucidating the release of cytokines from single cells as well as in vivo collection methods are described.

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“…In the sandwich assays such as cytokine and ELISA arrays, proteins captured on an antibody microarray are detected by a cocktail of detection antibodies, each antibody matched to one of the spotted antibodies. These sandwich-based arrays are extremely sensitive for low abundant cytokines or growth factors [112][113][114][115]. The correct use and interpretation of antibody microarray data requires proper normalization of the data.…”

Section: Protein Arraysmentioning

confidence: 99%

Human body fluid proteome analysis

2006

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The focus of this article is to review the recent advances in proteome analysis of human body fluids, including plasma/serum, urine, cerebrospinal fluid, saliva, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate fluid, tear fluid, and amniotic fluid, as well as its applications to human disease biomarker discovery. We aim to summarize the proteomics technologies currently used for global identification and quantification of body fluid proteins, and elaborate the putative biomarkers discovered for a variety of human diseases through human body fluid proteome (HBFP) analysis. Some critical concerns and perspectives in this emerging field are also discussed. With the advances made in proteomics technologies, the impact of HBFP analysis in the search for clinically relevant disease biomarkers would be realized in the future.

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Proteomic Analysis of Serum Cytokine Levels in Response to Highly Active Antiretroviral Therapy (HAART)

Cited by 17 publications

References 30 publications

The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women

The effect of HIV infection and HAART on inflammatory biomarkers in a population-based cohort of women

Bioanalytical chemistry of cytokines – A review

Human body fluid proteome analysis

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