Proteomic analysis of rat plasma with experimental autoimmune uveitis based on label-free liquid chromatography–tandem mass spectrometry (LC–MS/MS)

Guo, Dadong; Gu, Peiming; Liu, Zhengfeng; Tang, Kai; Du, Yi

doi:10.1016/j.jchromb.2014.11.015

Cited by 11 publications

(15 citation statements)

References 16 publications

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“…A), indicating the credibility of LC‐MS/MS determination. Importantly, the determination of C4, MBL2 achieves the same accuracy with the alterations of C3 and IL‐1RAcP in EAU rat plasma in our previous investigations .…”

Section: Discussionsupporting

confidence: 76%

“…However, in comparison with the EAU model, the pathogenesis of uveitis in patients accompanies by complex genetic backgrounds together with aberrant immune responses, and thus causing the differences between C4 levels. In our previous study [12], we also observed an elevated C3 level in EAU rat plasma, which has the same tendency to C4 level. Herein, both C4 and C3 are complement components related to immune systems, demonstrating that the high levels of complement components may be closely associated with the development of uveitis.…”

Section: Discussionsupporting

confidence: 72%

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Proteomic Profiling Analysis Reveals a Link between Experimental Autoimmune Uveitis and Complement Activation in Rats

Guo¹,

Tang

et al. 2017

Scand J Immunol

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Uveitis is an autoimmune disease that usually damages the vision function, leading to poor visual quality in patients. As an autoimmune ocular inflammatory disease, the pathogenesis of uveitis is associated with abnormal expression of some proteins and aberrant regulation of multiple signalling pathways. Nevertheless, the detailed mechanism remains unclear. In this study, we induced an experimental autoimmune uveitis (EAU) model in rats. We determined the levels of C3a and membrane attack complex C5b-9 (soluble C5b-9, sC5b-9) in both plasma and aqueous humour, identified the differentially expressed proteins in plasma by liquid chromatography-tandem mass spectrometry and employed bioinformatics algorithms to analyse differentially expressed proteins in EAU rat plasma. The results demonstrate that there were 168 differentially expressed plasma proteins in EAU rats versus control subjects. The levels of sC5b-9 and C3a were elevated in the plasmas and aqueous humours of EAU rats. Gene ontology enrichment analysis showed that the differentially expressed proteins in EAU rat plasma were mainly involved in metabolic and immune processes. Kyoto encyclopedia of genes and genomes (KEGG) pathway annotation, database for annotation, visualization and integrated discovery (DAVID) and protein-protein interaction analyses revealed that the differentially expressed proteins in EAU rat plasmas were closely associated with complement and coagulation cascades, metabolic pathways, NF-kappa B, PI3K-Akt, Toll-like receptors and autophagy. Overall, the differentially expressed proteins in EAU rat plasmas are mainly involved in the complement and coagulation cascades. The pathogenesis of uveitis closely correlates with complement activation.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionsupporting

confidence: 72%

Proteomic Profiling Analysis Reveals a Link between Experimental Autoimmune Uveitis and Complement Activation in Rats

Guo¹,

Tang

et al. 2017

Scand J Immunol

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“…ac indicated the categorization corresponding to upregulated miRNAs, and d-f indicated the categorization corresponding to downregulated miRNAs. The digit in the bracket was the amount of differentially expressed genes associated with the GO term D. Guo et al was either increased (e.g., mir-148b-3p, mir-20b-5p, mir-17-5p, mir-30b-5p) or decreased (e.g., mir-291a-5p, mir30b-3p), and we also found the aberrant expression of miRNAs accompanied by the abnormally expression of various proteins in serum in EAU rats [46]. Previous study has demonstrated that the mRNA levels of IL-17 and IFN-c were significantly upregulated in the retina in EAU rats.…”

Section: Discussionsupporting

confidence: 65%

Characterization of microRNA expression profiling in peripheral blood lymphocytes in rats with experimental autoimmune uveitis

Guo

Liu

et al. 2015

Inflamm. Res.

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“…Higher serum HPT levels were obtained in patients with BD compared to controls, and there is no obvious difference between the quiescent and active stages [43,44]. The plasma level of HPT increased signifcantly in EAU rats compared to controls [13].…”

Section: Prm Validationmentioning

confidence: 99%

Differential urine proteome analysis of a bovine IRBP induced uveitis rat model by discovery and parallel reaction monitoring proteomics

Qin

Wang

et al. 2019

Preprint

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Uveitis, a group of intraocular inflammatory diseases, is one of the major causes of severe visual impairment among working age population. Urine is a promising resource for biomarker research, which could sensitively reflect the changes of the body. This study was designed to explore urinary protein biomarkers for diagnosis and/or monitoring of uveitis. Experimental autoimmune uveitis (EAU) rat model induced by bovine interphotoreceptor retinoid-binding protein (IRBP) was used to mimic the uveitis. In the discovery phase, urine samples from EAU and control rats were analyzed by data independent acquisition (DIA) approach combined with high-resolution mass spectrometry. Overall, 704 high confidential proteins were identified, of which 100 were differentially expressed (37, 33, 37, and 44 on day 5, 8, 12, and 16, respectively, after bovine IRBP immunization) (1.5-fold change, P<0.05). Gene Ontology analysis of the dysregulated proteins showed that chronic inflammatory response, neutrophil aggregation and immune system processes were significantly enriched. Finally, parallel reaction monitoring (PRM) approach was used for further validation. A total of 12 urinary proteins (MMP8, NGAL, HPT, UROM, RISC, A1AG, TTHY, KNT1, C9, PTER, CBG, and FUCA1) changed significantly, even when there is no clinical manifestations and histopathological ocular damages in the EAU rats. Our findings represent the first step towards urinary protein diagnostic biomarkers for uveitis. Biological Significance:This is the first study investigating urinary protein candidate biomarkers for uveitis using data independent acquisition (DIA) combined with parallel reaction monitoring (PRM) in experimental autoimmune uveitis (EAU) rats. The results revealed that urine is a promising resource for early diagnostics of uveitis. Further research including clinical urine samples is needed to determine the sensitivity and specificity of these candidate biomarkers for uveitis.

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Proteomic analysis of rat plasma with experimental autoimmune uveitis based on label-free liquid chromatography–tandem mass spectrometry (LC–MS/MS)

Cited by 11 publications

References 16 publications

Proteomic Profiling Analysis Reveals a Link between Experimental Autoimmune Uveitis and Complement Activation in Rats

Proteomic Profiling Analysis Reveals a Link between Experimental Autoimmune Uveitis and Complement Activation in Rats

Characterization of microRNA expression profiling in peripheral blood lymphocytes in rats with experimental autoimmune uveitis

Differential urine proteome analysis of a bovine IRBP induced uveitis rat model by discovery and parallel reaction monitoring proteomics

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