Proteomic Analysis of Postsynaptic Protein Complexes Underlying Neuronal Plasticity

Baucum, II Anthony J.

doi:10.1021/acschemneuro.7b00008

Cited by 14 publications

(12 citation statements)

References 154 publications

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“…Sharing functions of up-regulated genes -binding, transporter and catalytic activities -were complemented by signal transducer activity in BA10L and by receptor activity in BA10R ( Figure 1A). GO analysis of protein classes demonstrated strong increase in diversity of protein classes up-regulated in BA10R compared to BA10L, extending the latter with calcium binding proteins, cell adhesion molecules, receptors, chaperones, extracellular matrix proteins and other classes known to be involved in neuronal plasticity (Baucum, 2017;Dzyubenko, Gottschling, & Faissner, 2016;Gyurko, Soti, Stetak, & Csermely, 2014;Sheng, Leshchyns'ka, & Sytnyk 2013) ( Figure 1B).…”

Section: Resultsmentioning

confidence: 97%

Exploring terra incognita of cognitive science: Lateralization of gene expression at the frontal pole of the human brain

Dolina¹,

Efimova²,

Kildyushov³

et al. 2017

Psych. Rus.

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Background. Rostral prefrontal cortex, or frontopolar cortex (FPC), also known as Brodmann area 10 (BA10), is the most anterior part of the human brain. It is one of the largest cytoarchitectonic areas of the human brain that has significantly increased its volume during evolution. Anatomically the left (BA10L) and right (BA10R) parts of FPC show slight asymmetries and they may have distinctive cognitive functions. Objective. In the present study, we investigated differential expression of the transcriptome in the left and right parts of BA10. Design. Postmortem samples of human brain tissue from fourteen donors (male/ female without history of psychiatric and neurological diseases, mean age 39.79±3.23 years old, mean postmortem interval 12.10±1.76 h) were obtained using the resources of three institutions: the Partner Institute of Computational Biology of Chinese Academy of Sciences, the Max Planck Institute for Evolutionary Anthropology, and NIH Neuro-BioBank. Results. By using a standard RNA-sequencing followed by bioinformatic analysis, we identified 61 genes with differential expression in the left and right FPC. In general, gene expression was increased in BA10R relative to BA10L: 40 vs. 21 genes, respectively. According to gene ontology analysis, the majority of up-regulated genes in BA10R belonged to the protein-coding category, whereas protein-coding and non-coding genes

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Section: Resultsmentioning

confidence: 97%

Exploring terra incognita of cognitive science: Lateralization of gene expression at the frontal pole of the human brain

Dolina¹,

Efimova²,

Kildyushov³

et al. 2017

Psych. Rus.

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“…The postsynaptic organization of synapses is very complex (Sheng & Kim, ). Based on large‐scale proteomic analysis, the PSD contains hundreds of proteins, among them different types of glutamate receptors, signaling molecules such as kinases (e.g., calcium/calmodulin‐dependent protein kinase II [CaMKII]) and phosphatases (such as phosphatase 1, 2A, 5, calcineurin), scaffolding and the adaptor proteins (such as PSD‐95, SAP‐102), regulatory proteins (such as transmembrane AMPAR‐regulatory proteins [TARPs]), adhesion molecules, cytoskeletal proteins (such as actin, tubulin, myosin), and multiple other proteins (Baucum 2nd, ; Collins et al, ; Lisman, Schulman, & Cline, ). Glutamate receptors assemble with signal transduction proteins into large multiprotein complexes, which are required for cellular signaling (Husi et al, ).…”

Section: Glutamatergic Synaptic and Extrasynaptic Signaling Between Nmentioning

confidence: 99%

Glutamatergic signaling between neurons and oligodendrocyte lineage cells: Is it synaptic or non‐synaptic?

Kula

Chen

Kukley

2019

Glia

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Fast chemical synaptic transmission is a major form of neuronal communication in the nervous system of mammals. Another important, but very different, form of intercellular communication is volume transmission, which is a slower non‐synaptic signaling. The amino acid glutamate is the most abundant excitatory neurotransmitter in the nervous system, which mediates both synaptic and non‐synaptic signaling via ionotropic and metabotropic glutamate receptors. Intriguingly, neurons establish glutamatergic synapses also with oligodendrocyte precursor cells (NG2+‐glia). Moreover, neuronal activity and glutamate receptors play an important role in the development and functionality of oligodendrocytes and their precursors in vivo. Yet, molecular characteristics and functional significance of neuron–glia synapses remain poorly understood, and it is unclear how glutamate receptors mediate the effects of neuronal activity on the oligodendrocyte lineage cells. In this review, we discuss what is known with regard to synaptic and non‐synaptic glutamatergic signaling between neurons and oligodendrocyte lineage cells, what can be suggested based on the current state of knowledge, and what is fully unknown and requires new research.

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“…Postsynaptic density protein of 95 kDa (PSD-95) is a scaffolding protein encoded by the disc large homolog 4 (DLG4) gene. It is highly enriched in the postsynaptic membrane and interacts with multiple synaptic proteins [ 1 ] through its three Post synaptic density protein, Drosophila disc large tumor suppressor, and Zonula occludens-1 protein (PDZ) domains. It plays an important role in synaptic plasticity [ 2 ], learning and memory [ 3 ], and other functions depending on the proteins it binds to.…”

Section: Main Textmentioning

confidence: 99%

3D imaging of PSD-95 in the mouse brain using the advanced CUBIC method

Liang

Wang

et al. 2018

Mol Brain

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AimsPostsynaptic density – 95 kDa protein (PSD95) is an important molecule on the postsynaptic membrane. It interacts with many other proteins and plays a pivotal role in learning and memory formation. Its distribution in the brain has been studied previously using in situ hybridization as well as immunohistochemistry. However, these studies are based on 2 dimensional (2D) sections and results are presented with a few sections. The present study aims to show PSD-95 distribution in 3 dimensions (3D) without slicing the brain tissue of C57BL/6 mice into sections using the advanced CUBIC technique.MethodsImmunofluorescent staining using a PSD-95 antibody was performed on a half of the mouse brain after clarifying it using the advanced CUBIC protocol. The brain tissue was imaged using a Zeiss Z1 light sheet microscope and 3D reconstruction was completed using the Arivis Vision 4 dimensional (4D) software.ResultsThe majority of brain nuclei have similar distribution pattern to what has been reported from in situ hybridization and immunohistochemical studies in the mouse. The signal can be easily followed in the 3D and their spatial relationship with adjacent structures clearly demarcated. In the present study, some fiber bundles also showed strong PSD-95 signal, which is different from what was shown in previous studies and need to be confirmed in future studies.Electronic supplementary materialThe online version of this article (10.1186/s13041-018-0393-4) contains supplementary material, which is available to authorized users.

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Proteomic Analysis of Postsynaptic Protein Complexes Underlying Neuronal Plasticity

Cited by 14 publications

References 154 publications

Exploring terra incognita of cognitive science: Lateralization of gene expression at the frontal pole of the human brain

Exploring terra incognita of cognitive science: Lateralization of gene expression at the frontal pole of the human brain

Glutamatergic signaling between neurons and oligodendrocyte lineage cells: Is it synaptic or non‐synaptic?

3D imaging of PSD-95 in the mouse brain using the advanced CUBIC method

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