Proteomic analysis of pathogen-responsive proteins from maize stem apoplast triggered by Fusarium verticillioides

Haseeb, Hafiz Abdul; Zhang, Jun; Guo, Yushuang; Gao, Mengxiang; Guo, Wei

doi:10.1016/s2095-3119(21)63657-2

Cited by 6 publications

(4 citation statements)

References 65 publications

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“…Moderate expression was noted at 48 hpi where ZmGLP4-2 , ZmGLP4-3 , ZmGLP4-4 , ZmGLP4-8 , ZmGLP4-12 , ZmGLP4-15 , ZmGLP4-19 , ZmGLP4-20 , ZmGLP10-1 , ZmGLP10-2 , ZmGLP10-3 , ZmGLP10-4 , and ZmGLP10-5 were mostly active, while no expression was observed at 4, 12, and 24 hpi. Previously, few GLPs showed expression at 7 days postinteraction with Fusarium verticillioides in maize . In another study, a few GLPs along with other defense proteins were upregulated after Fusarium graminearum inoculation .…”

Section: Resultsmentioning

confidence: 85%

“…Previously, few GLPs showed expression at 7 days postinteraction with Fusarium verticillioides in maize. 114 In another study, a few GLPs along with other defense proteins were upregulated after Fusarium graminearum inoculation. 115 A similar study conducted in wheat showed GLPs being the main player in defense against F. graminearum .…”

Section: Resultsmentioning

confidence: 95%

“…Similarly, the highest expression was noted at 4, 7, and 10 dpi (days postinfection), while the minimal response was noted at 0 and 2 dpi, but no expression was noted at 14 dpi. Till now, no significant data are available regarding the use of GLPs against F. virguliforme , but reports can be found against Fusarium solani , 96 , 120 Fusarium oxysporum , 22 , 121 Fusarium graminearum , 115 and Fusarium verticillioides , ( 114 ) Similarly, GhABP19 regulates resistance against Verticillium ( Verticillium dahliae ) and Fusarium wilt ( Fusarium oxysporum ) in plants via SOD activity-regulating JA pathways in cotton. 22 A similar observation was noted for 11 GhGLP genes against Verticillium dahliae -infected Gossypium barbadense .…”

Section: Resultsmentioning

confidence: 99%

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Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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Germin (GER) and germin-like proteins (GLPs) play an important role in various plant processes. Zea mays contains 26 germin-like protein genes ( ZmGLPs ) located on chromosomes 2, 4, and 10; most of which are functionally unexplored. The present study aimed to characterize all ZmGLPs using the latest computational tools. All of them were studied at a physicochemical, subcellular, structural, and functional level, and their expression was predicted in plant development, against biotic and abiotic stresses using various in silico approaches. Overall, ZmGLPs showed greater similarity in their physicochemical properties, domain architecture, and structure, mostly localized in the cytoplasmic or extracellular regions. Phylogenetically, they have a narrow genetic background with a recent history of gene duplication events on chromosome 4. Functional analysis revealed novel enzymatic activities of phosphoglycolate phosphatase, adenosylhomocysteinase, phosphoglycolate phosphatase-like, osmotin/thaumatin-like, and acetohydroxy acid isomeroreductase largely mediated by disulfide bonding. Expression analysis revealed their crucial role in the root, root tips, crown root, elongation and maturation zones, radicle, and cortex with the highest expression being observed during germination and at the maturity levels. Further, ZmGLPs showed strong expression against biotic ( Aspergillus flavus , Colletotrichum graminicola , Cercospora zeina , Fusarium verticillioides , and Fusarium virguliforme ) while limited expression was noted against abiotic stresses. Concisely, our results provide a platform for additional functional exploration of the ZmGLP genes against various environmental stresses.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

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Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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“… Cheng et al (2009) investigated the secretory proteome of suspension-cultured Arabidopsis cells in response to SA treatment, and found 63 secreted proteins were induced by SA. In maize, the apoplast proteins responsive to Fusarium verticillioides are related to signal transduction, cell wall modifications, carbohydrate metabolism, and cell redox homeostasis ( Hafiz et al, 2022 ). Guerra-Guimaraes et al (2015) investigated the apoplastic proteome of coffee plants infected with Hemileia vastatrix , and identified numerous resistance-related proteins, including pathogen related-like proteins (PR-proteins), serine proteases, and glycohydrolases in the cell wall.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance in Arabidopsis thaliana

Jiang,

Pan,

Zhou

et al. 2023

PeerJ

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Background Plant-pathogen interactions occur in the apoplast comprising the cell wall matrix and the fluid in the extracellular space outside the plasma membrane. However, little is known regarding the contribution of the apoplastic proteome to systemic acquired resistance (SAR). Methods Specifically, SAR was induced by inoculating plants with Pst DC3000 avrRps4. The apoplast washing fluid (AWF) was collected from the systemic leaves of the SAR-induced or mock-treated plants. A label free quantitative proteomic analysis was performed to identified the proteins related to SAR in AWF. Results A total of 117 proteins were designated as differentially accumulated proteins (DAPs), including numerous pathogenesis-related proteins, kinases, glycosyl hydrolases, and redox-related proteins. Functional enrichment analyses shown that these DAPs were mainly enriched in carbohydrate metabolic process, cell wall organization, hydrogen peroxide catabolic process, and positive regulation of catalytic activity. Comparative analysis of proteome data indicated that these DAPs were selectively enriched in the apoplast during the induction of SAR. Conclusions The findings of this study indicate the apoplastic proteome is involved in SAR. The data presented herein may be useful for future investigations on the molecular mechanism mediating the establishment of SAR.

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Elucidating the mechanisms of Fusarium oxysporum f. sp. tuberosi inhibition using functionalized multi-walled carbon nanotubes: A comprehensive analysis of biophysical and molecular interactions1

Manzoor,

Irshad,

Azam

et al. 2024

Journal of Integrative Agriculture

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Proteomic analysis of pathogen-responsive proteins from maize stem apoplast triggered by Fusarium verticillioides

Cited by 6 publications

References 65 publications

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance in Arabidopsis thaliana

Elucidating the mechanisms of Fusarium oxysporum f. sp. tuberosi inhibition using functionalized multi-walled carbon nanotubes: A comprehensive analysis of biophysical and molecular interactions1

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