Proteomic analysis of papaya fruit ripening using 2DE-DIGE

Nogueira, Silvia Beserra; Labate, Carlos Alberto; Gozzo, Fábio C.; Pilau, Eduardo Jorge; Lajolo, Franco Maria; Nascimento, João Roberto Oliveira do

doi:10.1016/j.jprot.2011.11.015

Cited by 66 publications

(54 citation statements)

References 37 publications

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“…To our knowledge, previously published studies have used gel (1-and 2-DE and DIGE)-based separations of C. papaya proteins followed by peptide mass spectrometry analysis. In summary, the total number of identified proteins per sample type is 76 from somatic embryos [31], 54 from papaya fruit pulp [32,33], 71 from leaves [22], 186 from latex [6,34] and 1,581 proteins from isolated chromoplasts [35]. In this study, field-grown pre-flowering C. papaya plants' leaf samples were submitted to protein extraction and in-solution digestion, and the resulting peptides were analyzed using an LC-MS/MS based approach.…”

Section: Discussionmentioning

confidence: 99%

Label-free quantitative proteomic analysis of pre-flowering PMeV-infected Carica papaya L.

Soares

Werth

Madroñero

et al. 2017

Journal of Proteomics

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Section: Discussionmentioning

confidence: 99%

Label-free quantitative proteomic analysis of pre-flowering PMeV-infected Carica papaya L.

Soares

Werth

Madroñero

et al. 2017

Journal of Proteomics

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“…Moreover, when the results were compared to those of papaya fruit exposed to radiation (GOMEz et al, 2002), the same conclusion of an operative sucrose synthesis during ripening can be achieved. Because the trace amounts of starch in the flesh of papayas could not account for the accumulation of soluble sugars, it is possible to speculate that some mechanisms of cell wall disassembly (NoGUEIRA et al, 2012;RAZALI et al, 2013) could provide a source of carbon for sugar synthesis during ripening, including sucrose. The similar profiles for glucose and fructose could be an indication that those sugars come from the accumulated sucrose, especially by the action of invertases in fully ripe fruits (PAULL et al, 2011).…”

Section: Biochemical Parameters 24 H Control and 1-mcp Treatmentsmentioning

confidence: 99%

Influence of 1-Methylcyclopropene on the Biochemical Response and Ripening of ‘Solo’ Papayas

et al. 2016

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abstract-The market demand for tropical fruits has been growing steadily over the past two decades and global papaya production has grown significantly over the last few years. This sector, however, suffers greatly from postharvest losses due to reduced quantity and quality of fruits between harvest and consumption. The use of ethylene inhibitors after harvest could improve the final quality of the fruit to satisfy the consumer and also minimize waste. The physiological and biochemical responses of 'Solo' papayas treated with the ethylene inhibitor 1-methylcyclopropene (1-MCP) to extend storage shelf life and maintain quality during long-term storage are deeply discussed in this study. Papaya fruits arrived at Cranfield University (CU) and received a 24 h 1-MCP, being stored at 20 ºC for 10 days. The ethylene inhibitor 1-MCP application significantly delayed 'Solo' papaya ripeness on fruit storage by reducing respiration rate and ethylene production. There was a delay from 7 days in fruit firmness loss and the retention of green peel colour was increased. Inhibition of ethylene perception by 1-MCP did not prevent the accumulation of sugars and the mean values were similar and higher than those found for control fruits, which are possibly due to the lower reaction speed, leading to a higher accumulation. Index terms:Carica papaya L., fruit quality, fruit waste, 1-MCP, tropical fruits.InfluÊncIa do 1-metIlcIclopropeno sobre a resposta bIoQuÍmIca e amadurecImento de mamÕes 'solo' resumo-O mercado de frutos tropicais cresceu constantemente ao longo das duas últimas décadas e a produção mundial de mamão tem crescido significativamente nos últimos anos. No entanto, esse setor sofre muito com as perdas pós-colheita devido à reduzida quantidade e qualidade dos frutos entre a colheita e o consumo. A utilização de inibidores de etileno após a colheita poderia melhorar a qualidade final do fruto para satisfazer o consumidor e também minimizar o desperdício. As respostas fisiológicas e bioquímicas de mamões 'Solo' tratados com o inibidor de etileno 1-metilciclopropeno (1-MCP) para prolongar a vida útil e manter a qualidade durante o armazenamento a longo prazo são detalhadamente discutidas neste estudo. os frutos chegaram à Universidade de Cranfield (UC) e receberam tratamento com 1-MCP por 24 h, sendo armazenados a 20 ºC durante 10 dias. A aplicação do inibidor de etileno 1-MCP retardou significativamente o amadurecimento de mamões 'Solo' no armazenamento dos frutos, reduzindo a taxa de respiração e a produção de etileno. Houve um atraso de 7 dias na perda de firmeza e a retenção da cor verde da casca dos frutos foi aumentada. A inibição da percepção de etileno pelo 1-MCP não impediu o acúmulo de açúcares e os valores médios foram semelhantes e superiores aos encontrados para as frutas do tratamento controle, que são possivelmente devido à menor velocidade de reação, levando a um maior acúmulo. termos para indexação:Carica papaya L., qualidade, desperdício, 1-MCP, frutos tropicais.ISSn 0100-2945 http://dx

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“…In the fruit-ripening studies for papaya, six main categories such as cell wall synthesis, ethylene biosynthesis, climacteric respiratory burst, stress response, synthesis of carotenoid precursors and chromoplast differentiation were shown to be related to fruit ripening using 2DE-DIGE (Nogueira et al 2012 ). Traditional 2D PAGE showed several cell wall-degrading enzymes also related to fruit ripening in papaya (Huerta-Ocampo et al 2012 ).…”

Section: Vegetable Crop Proteomicsmentioning

confidence: 99%

Translating the Genome for Translational Research: Proteomics in Agriculture

Caguioa

Raorane

Kohli

2015

Plant Biology and Biotechnology

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The increasing number of novel tools, methods and capacities to elucidate and understand the genome of various organisms is steadily leading the path for similar groundbreaking studies in downstream comprehension of the genomes. Platforms for analytical and functional transcriptomics have seen a similar surge in operational outputs. The cryptic complexity of the DNA in the genomes is manifested in various forms and functions of the RNA it codes for. Such complexity reaches much more elaborate intricacies in the translational protein products of the RNA. Although transcripts regulate a number of biological processes, proteins form the basic functional unit of most procedures that are evinced as a phenotype. Progressively increasing realization that there is limited correspondence between the level of a specifi c transcript and protein is only the beginning of appreciating that predictive biology would be less dependent on transcripts than on proteins. Principles of protein function that were explored much before the DNA and RNA paradigms are coming back to inform the omics world in the functional and regulatory capacity of proteins. Combined with the evolution of the high-throughput analytical capacity for proteins, these are clubbed into what is called proteomics, which is also riding the wave of computational and electronic revolutions in biology. Using such omic technologies for agriculture however lags behind compared to their use in medicine. Nevertheless, the recent appreciation of the need for food and feed security under the predicted climatodemographic scenarios is pushing the frontiers of agricultural research. The use of proteomics in particular is gaining ground rather quickly, and an increased understanding of crop phenotype as related to proteomics is

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Proteomic analysis of papaya fruit ripening using 2DE-DIGE

Cited by 66 publications

References 37 publications

Label-free quantitative proteomic analysis of pre-flowering PMeV-infected Carica papaya L.

Label-free quantitative proteomic analysis of pre-flowering PMeV-infected Carica papaya L.

Influence of 1-Methylcyclopropene on the Biochemical Response and Ripening of ‘Solo’ Papayas

Translating the Genome for Translational Research: Proteomics in Agriculture

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