Label-free quantitative proteomic analysis of pre-flowering PMeV-infected Carica papaya L.

Soares, Eduardo de Almeida; Werth, Emily G.; Madroñero, Johana; Ventura, José Aires; Rodrigues, Silas P.; Hicks, Leslie M.; Fernandes, Patrícia Machado Bueno

doi:10.1016/j.jprot.2016.06.025

Cited by 14 publications

(16 citation statements)

References 49 publications

(61 reference statements)

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“…However, based on the unaltered cross-linking profile upon ligand binding shown in Table 1, major changes in the accessibility is unlikely for these lysine residues. Among a total of 25 cross-links across the entire GPR110 structure, only the cross-linking of K398-K438 in the GAIN domain, and the inter-domain cross-linking of K783-K852 in the intracellular regions, increased significantly after stimulation with synaptamide compared to the case with the OEAor DMSO-treated control (fold-change ≥ 1.5 and p ≤ 0.05) 30,31 . As the accessibility of K398, K438, K783, and presumably K852 remained unchanged, changes in the cross-linking profile for K398-K438 and K783-K852 are most likely due to changes in proximity between the cross-linked pairs.…”

Section: Resultsmentioning

confidence: 95%

“…1, GPR110overexpressing cells were stimulated with synaptamide for 10 min for GPR110 activation, while DMSO (vehicle) or oleoylethanolamine (OEA), an inactive structural analog of synaptamide, was used in parallel as an unstimulated control 20 . The ligand-induced changes in GPR110 conformation were deduced from comparative analysis of the cross-linked peptides and monolinks using label-free quantitation 30,31 (Table 1, Supplementary Tables 2, 3). A total of 13 monolinks were detected, including K398 and K438 in the GAIN domain, and K783 at TM6.…”

Section: Resultsmentioning

confidence: 99%

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Synaptamide activates the adhesion GPCR GPR110 (ADGRF1) through GAIN domain binding

Huang

Kwon

et al. 2020

Commun Biol

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Adhesion G protein-coupled receptors (aGPCR) are characterized by a large extracellular region containing a conserved GPCR-autoproteolysis-inducing (GAIN) domain. Despite their relevance to several disease conditions, we do not understand the molecular mechanism by which aGPCRs are physiologically activated. GPR110 (ADGRF1) was recently deorphanized as the functional receptor of N-docosahexaenoylethanolamine (synaptamide), a potent synaptogenic metabolite of docosahexaenoic acid. Thus far, synaptamide is the first and only smallmolecule endogenous ligand of an aGPCR. Here, we demonstrate the molecular basis of synaptamide-induced activation of GPR110 in living cells. Using in-cell chemical cross-linking/ mass spectrometry, computational modeling and mutagenesis-assisted functional assays, we discover that synaptamide specifically binds to the interface of GPR110 GAIN subdomains through interactions with residues Q511, N512 and Y513, causing an intracellular conformational change near TM6 that triggers downstream signaling. This ligand-induced GAIN-targeted activation mechanism provides a framework for understanding the physiological function of aGPCRs and therapeutic targeting in the GAIN domain.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Synaptamide activates the adhesion GPCR GPR110 (ADGRF1) through GAIN domain binding

Huang

Kwon

et al. 2020

Commun Biol

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“…In Latin-America, proteomics is less addressed; basically, the country that is leading the research studies on structural, expression and functional proteomics in plant-virus interactions is Brazil. Some examples of researches conducted in Brazil are the study of the chloroplast proteomic profile in Tomato blistering mosaic virus (ToBMV) and Nicotiana benthamiana interaction (Megias et al, 2018), the host proteomic response to Citrus tristeza virus (Dória and Pirovani, 2019) and to the Papaya meleira virus complex (PMeV) (Soares et al, 2017). In Colombia, however, similarly to transcriptomics, proteomics has been little explored.…”

Section: Proteomics To Better Understand Plant-virus Interactionsmentioning

confidence: 99%

Next generation sequencing and proteomics in plant virology: how is Colombia doing?

et al. 2019

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Crop production and trade are two of the most economically important activities in Colombia, and viral diseases cause a high negative impact to agricultural sector. Therefore, the detection, diagnosis, control, and management of viral diseases are crucial. Currently, Next-Generation Sequencing (NGS) and ‘Omic’ technologies constitute a right-hand tool for the discovery of novel viruses and for studying virus-plant interactions. This knowledge allows the development of new viral diagnostic methods and the discovery of key components of infectious processes, which could be used to generate plants resistant to viral infections. Globally, crop sciences are advancing in this direction. In this review, advancements in ‘omic’ technologies and their different applications in plant virology in Colombia are discussed. In addition, bioinformatics pipelines and resources for omics data analyses are presented. Due to their decreasing prices, NGS technologies are becoming an affordable and promising means to explore many phytopathologies affecting a wide variety of Colombian crops so as to improve their trade potential.

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“…Another comparative proteomic analysis showed that certain proteins, such as enolase, esterase and ADH3, show an essential role in maturation of somatic papaya embryo cells [26]. Recently, a differential proteome of virus-infected pre-flowering C. papaya vs. uninfected plants was published [27]. In our study, we employed an integration of the basic HPLC fractionation and LC–MS/MS approach to identify a total of 2818 differential accumulated proteins (DAPs) of papaya fruit during ripening.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analysis provides novel insights into the regulation mechanism underlying papaya (Carica papaya L.) exocarp during fruit ripening process

Bian

et al. 2019

BMC Plant Biol

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Background Papaya ( Carica papaya L.) is a popular climacteric fruit, undergoing various physico-chemical changes during ripening. Although papaya is widely cultivated and consumed, few studies on the changes in metabolism during its ripening process at the proteasome level have been performed. Using a newly developed TMT-LCMS analysis, proteomes of papaya fruit at different ripening stages were investigated. Results In total, 3220 proteins were identified, of which 2818 proteins were quantified. The differential accumulated proteins (DAPs) exhibited various biological functions and diverse subcellular localizations. The KEGG enrichment analysis showed that various metabolic pathways were significantly altered, particularly in flavonoid and fatty acid metabolisms. The up-regulation of several flavonoid biosynthesis-related proteins may provide more raw materials for pigment biosynthesis, accelerating the color variation of papaya fruit. Variations in the fatty acid metabolism- and cell wall degradation-related proteins were investigated during the ripening process. Furthermore, the contents of several important fatty acids were determined, and increased unsaturated fatty acids may be associated with papaya fruit volatile formation. Conclusions Our data may give an intrinsic explanation of the variations in metabolism during the ripening process of papaya fruit. Electronic supplementary material The online version of this article (10.1186/s12870-019-1845-4) contains supplementary material, which is available to authorized users.

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Label-free quantitative proteomic analysis of pre-flowering PMeV-infected Carica papaya L.

Cited by 14 publications

References 49 publications

Synaptamide activates the adhesion GPCR GPR110 (ADGRF1) through GAIN domain binding

Synaptamide activates the adhesion GPCR GPR110 (ADGRF1) through GAIN domain binding

Next generation sequencing and proteomics in plant virology: how is Colombia doing?

Comparative proteomic analysis provides novel insights into the regulation mechanism underlying papaya (Carica papaya L.) exocarp during fruit ripening process

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