Proteomic Analysis of Oil Mobilization in Seed Germination and Postgermination Development of Jatropha curcas

Yang, Mo; Liu, Yujun; Liu, Yun; Chen, Hui; Cao, Fan; Shen, Shihua

doi:10.1021/pr800799s

Cited by 97 publications

(73 citation statements)

References 40 publications

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“…Recently, candidate genes involved in fatty acid biosynthesis, biotic and abiotic stress tolerance mechanisms, and regulation of secondary metabolites and phorbol esters, which are considered as important breeding targets, have been identified from whole genome sequencing analyses (Sato et al 2011), proteomics (Yang et al 2009) and transcriptomics studies (Costa et al 2010;Natarajan et al 2010;Natarajan and Parani 2011). Knowledge of their molecular functions will accelerate the process of molecular breeding of Jatropha.…”

Section: Genetic Engineering Is a Practical Technique To Improvementioning

confidence: 99%

Establishment of bispyribac selection protocols for Agrobacterium tumefaciens- and Agrobacterium rhizogenes-mediated transformation of the oil seed plant Jatropha curcas L.

Kajikawa

Morikawa

Inoue

et al. 2012

Plant Biotechnology

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Cultivation of the biofuel plant Jatropha (Jatropha curcas L.) has spread around the world because of its drought resistance, high seed oil content, and adaptability to di erent environmental conditions. Because of these attributes, Jatropha has the potential to be one of the main resources for next-generation biodiesel fuel. To improve the productivity of Jatropha biomass, it is important to understand the molecular functions of key Jatropha genes, and to modify various agronomic traits of Jatropha via molecular breeding. A reliable and e cient protocol for genetic transformation of Jatropha is a prerequisite for molecular biology research and breeding on this plant. Here, we developed a system in which the herbicide bispyribac sodium salt, which inhibits acetolactate synthase, was used as the selection agent, and a two-point-mutated acetolactate synthase gene (mALS) was used to confer resistance upon transformants. Application of this system signi cantly improved the e ciency of Agrobacterium tumefaciens-mediated stable transformation of the high-yielding elite Jatropha population, IP-2P. e bispyribac-mALS system was also successfully applied in the Agrobacterium rhizogenes-mediated hairy roots system, which allowed integration of a foreign gene and expression in Jatropha root tissues within 2 weeks. e new protocols described here are powerful tools not only for functional studies on endogenous genes, but also for the molecular breeding of Jatropha to develop elite varieties.

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Section: Genetic Engineering Is a Practical Technique To Improvementioning

confidence: 99%

Establishment of bispyribac selection protocols for Agrobacterium tumefaciens- and Agrobacterium rhizogenes-mediated transformation of the oil seed plant Jatropha curcas L.

Kajikawa

Morikawa

Inoue

et al. 2012

Plant Biotechnology

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show abstract

“…Aunque en los últimos diez años se ha incrementado sustancialmente el número de investigaciones con la planta J. curcas (Achten et al, 2010;Carels, 2013), impulsado por el interés en la obtención de materia prima para fabricar biocombustibles, se ha dado poca atención al estudio microscópico de los oleosomas de la semilla. Los estudios de proteómica de los oleosomas de J. curcas son abundantes Ming-Feng et al, 2009;Mao-Sheng et al, 2011;Popluechai et al, 2011), sin embargo, solo dos reportes se enfocaron en el análisis de los oleosomas por sí mismos (Gu et al, 2012;Ishii et al, 2013).…”

Section: Discussionunclassified

Oleosomas de semillas de Jatropha curcas L. como estimadores de diversidad en poblaciones del Sur de México

Adriano-Anaya¹,

Gómez-Pérez²,

Ruiz-González³

et al. 2014

Grasas y Aceites

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SUMMARY:Seed oleosomes of Jatropha curcas L. as diversity estimators in populations of Southern Mexico. In this work, we used oleosome data to estimate the variability in eight J. curcas populations of Southern Mexico, using a multivariate approach. We found spherical oleosomes with diameters between 2.18 μm and 4.15 μm, which have two types of spatial arrangements: clustered and dispersed. A discriminant analysis showed that populations with more differentiation are Oaxaca and Veracruz, while Isthmus was the most diverse. The most informative variables for their contribution to the discriminant functions were oleosome size and number of oleosomes·μL of tissue −1. A heritability study with seeds harvested in two consecutive years showed that the variables oleosomes·μL of tissue −1 and oleosomes per cell have high broad sense heritabilities (h 2 bs =94.3% and h 2 bs =92.2%, respectively). A cluster analysis revealed high levels of dissimilarity between individuals. Correlation was found between the ratio oleosomes/tissue (volume/volume) and total oil content of the seed. The identification of genotypes with contrasting characteristics could provide the basis for future studies of the inheritance of characters in J. curcas. KEYWORDS: Diversity; Heritability; Jatropha; Oleosomes; Populations; Variability RESUMEN: Se estudiaron los caracteres de oleosomas de la planta para biocombustibles Jatropha curcas L. y se estimó la diversidad de ocho poblaciones del sur de México. Se encontraron oleosomas esféricos, con diámetros entre 2.18 μm y 4.15 μm, presentando dos tipos de arreglos espaciales: agrupados y dispersos. Un análisis discriminante mostró que las poblaciones más diferenciadas del resto son Oaxaca y Veracruz, mientras que Istmo fue la más diversa. Las variables más informativas por su contribución a las funciones discriminantes fueron tamaño de oleosoma y número de oleosomas·μL de tejido −1 . En un estudio de heredabilidad con semillas cosechadas en dos años consecutivos se encontró que las variables oleosomas·μL de tejido −1 y oleosomas por célula tienen muy altas heredabilidades en sentido amplio (h 2 bs =94.3% y h 2 bs =92.2%, respectivamente). Un análi-sis de conglomerados reveló altos niveles de disimilitud entre individuos. Los análisis de asociación de variables encontraron correlación entre la relación porcentual oleosomas/tejido (volumen/volumen) y el contenido total de aceite de la semilla.

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“…A recent study to understand oil mobilization in germinating seeds, proteomic analysis of endosperm clearly showed that oil mobilization that was initiated during germination was used up for early seedling development [50]. Several pathways including b-oxidation, glyoxylate cycle, glycolysis, citric acid cycle, gluconeogenesis, and pentose phosphate pathway were involved in the oil mobilization.…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Jatropha curcas: Approaches to Engineer Fatty Acid Synthesis and Abiotic Stress Tolerance

Eswaran¹,

Johnson²

2012

Improving Crop Resistance to Abiotic Stress

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Proteomic Analysis of Oil Mobilization in Seed Germination and Postgermination Development of Jatropha curcas

Cited by 97 publications

References 40 publications

Establishment of bispyribac selection protocols for <i>Agrobacterium tumefaciens</i>- and <i>Agrobacterium rhizogenes</i>-mediated transformation of the oil seed plant <i>Jatropha curcas</i> L.

Establishment of bispyribac selection protocols for <i>Agrobacterium tumefaciens</i>- and <i>Agrobacterium rhizogenes</i>-mediated transformation of the oil seed plant <i>Jatropha curcas</i> L.

Oleosomas de semillas de <em>Jatropha curcas</em> L. como estimadores de diversidad en poblaciones del Sur de México

Jatropha curcas: Approaches to Engineer Fatty Acid Synthesis and Abiotic Stress Tolerance

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