Proteomic analysis of intestinal tissues from mice fed with Lentinula edodes-derived polysaccharides

Abstract

Lentinula edodes-derived polysaccharides are well known for their immunomodulation and antitumor activities. However, the mechanisms of action have not been fully elucidated. This study presents proteomic analysis of the colon and small intestine from mice fed with an immunostimulating heteropolysaccharide L2 from the fruit body of L. edodes. Two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF MS/MS were employed to characterize the protein profiles. Twenty nine gel spots representing 20 proteins in colon tissues and 38 gel spots in small intestine tissues representing 23 proteins were identified as showing significant changes in abundance. These differential proteins in abundance are mainly involved in metabolism, binding, structural components, and response to stimulus. Protein-protein interaction network analysis demonstrated mapping of the 20 colon proteins to a 7-protein and a 3-protein sub-network, and mapping of the 23 small intestine proteins to a 9-protein and a 5-protein sub-network. All the 40 altered proteins were integrated into a unified network containing 25 proteins, suggesting the existence of a concerted mechanism, although acting on the colon and small intestine separately. These findings facilitate the understanding of the regulatory mechanism in response to L2 treatment.

Proteomics analysis of chicken peripheral blood lymphocyte in Taishan Pinus massoniana pollen polysaccharide regulation

“…Hsp70 has been reported to exhibit adjuvant activity by promoting adaptive immune response to antigenic cells [21]. Xu et al’s proteomic study showed that Hsp70 and Hsp 90-beta were up-regulated and down-regulated, respectively, in intestinal tissues harvested from mice fed with Lentinula edodes -derived polysaccharides compared to the control group that received a normal diet [22]. Interestingly, the authors also observed a lower level of Annexin A2 in the polysaccharide group, which mirrored the finding in our current study.…”

iTRAQ-based Quantitative Proteomic Profiling of the Regulatory Mechanisms on Immune Functions and Blood Lipids in Aged Mice Fed with Lentinula edodes-Derived Polysaccharides

“…Three jejunum tissue samples were randomly selected from each O group and OL2 group. Protein extraction was conducted according to the previous method with slight modifications [21,23]. In brief, about 100 mg tissue samples were ground rapidly into powders in liquid nitrogen in a mortar.…”

“…In our previous study, a heteropolysaccharide consisting of glucose, galactose, and arabinose with an average molecular weight of 26 kDa, named L2, extracted from the fruit body of Lentinula edodes [18], exhibited immunoenhancing actions and altered the microbial community along the intestinal tract in adult mice [19,20]. Two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry (MS)-based proteomic study and real time (RT)-PCR analysis revealed that metabolism, binding, structural components, and response to stimulus might participate in L2-induced regulatory mechanisms [20,21]. Moreover, L2 induced greater impacts on the small intestinal region than on the colon regarding protein and gene expression levels in adult mice [20,21].…”

“…Two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry (MS)-based proteomic study and real time (RT)-PCR analysis revealed that metabolism, binding, structural components, and response to stimulus might participate in L2-induced regulatory mechanisms [20,21]. Moreover, L2 induced greater impacts on the small intestinal region than on the colon regarding protein and gene expression levels in adult mice [20,21]. Interestingly, L2 could partially restore the age-related immune function declines and reverse the age-altered gut microbiota composition in aged mice [22], demonstrating the anti-aging potential of L2.…”

1H NMR-based metabolic investigation of the effect ofLentinula edodes-derived polysaccharides on aged mice

“…Previous studies reported that one type of L. edodes ‐derived polysaccharide, named L2, showed immune‐stimulating activity in vitro and enhanced immune responses in vivo characterized by alterations in the gut microbiota and differential expressions in genes and proteins in the gut of adult mice (Xu, Yan, & Zhang, ; Xu, Yang, Ning, & Zhang, ; Xu & Zhang, ). L2 also exhibited anti‐ageing effects by partially restoring age‐attenuated immune functions and reversing the age‐altered gut microbial communities (Xu, Yang, Ning, & Zhang, ).…”