Proteomic analysis of hepatitis B virus‐associated hepatocellular carcinoma: Identification of potential tumor markers

Li, Chen; Tan, Ye-Xiong; Zhou, Hu; Ding, Shi‐Jian; Li, Su-Jun; Ma, Dongzhu; Man, Xiao-hua; Hong, Yi; Zhang, Lei; Li, Long; Xia, Qi-Chang; Wu, Jiarui; Wang, Hongyang; Zeng, Rong

doi:10.1002/pmic.200401141

Cited by 105 publications

(86 citation statements)

References 54 publications

(22 reference statements)

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“…(38,(40)(41)(42)(43)(44)(45)(46)(47)(48)(49) However, with the exception of vinculin (14,15) the genes reported to be associated with early recurrence of HCC in transcriptomic studies were not identified in this study, a fact possibly due to the current detection limitations of 2D-DIGE using the CyDye DIGE Fluor minimal dye. To improve the sensitivity of our proteomic studies, we are now considering the use of prefractionation methods, narrow range IPG gels, more sensitive fluorescent dye such as the CyDye DIGE Fluor saturation dye, and larger format 2D gels.…”

Section: Discussioncontrasting

confidence: 51%

Protein expression associated with early intrahepatic recurrence of hepatocellular carcinoma after curative surgery

et al. 2007

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The poor prognosis of patients with hepatocellular carcinoma (HCC) is attributed to intrahepatic recurrence. To understand the molecular background of early intrahepatic recurrence, we conducted a global protein expression study. We compared the protein expression profiles of the primary HCC tissues of 12 patients who showed intrahepatic recurrence within 6 months post surgery with those of 15 patients who had no recurrence 2 years post surgery. Two-dimensional difference gel electrophoresis identified 23 protein spots, the intensity of which was highly associated with early intrahepatic recurrence. To validate the prediction performance of the identified proteins, we examined additional HCC tissues from 13 HCC patients; six with early intrahepatic recurrence and seven without recurrence. We found that all but one of the 13 patients were grouped according to their recurrence status based on the intensity of the 23 protein spots. D espite recent progress in early diagnostic modalities and therapeutic management, the prognosis of hepatocellular carcinoma (HCC) patients remains poor, mainly due to intrahepatic recurrence.(1) The incidence of intrahepatic recurrence in the liver remnant ranges from 50% to 100% of HCC patients who undergo curative resection as a result of either intrahepatic metastasis from the primary tumor or multicentric recurrence (2)(3)(4)(5)(6) and the median survival after recurrence is only 11 months. Although various molecular alterations have been correlated with early recurrence of HCC (8)(9)(10)(11)(12)(13) and studies on such events furthered our understanding of HCC biology, the underlying mechanisms remain obscure. Studies focusing on individual candidate genes may be insufficient for precisely understanding the background of the malignant behavior of tumor cells, because the features of cancer cells are defined by multiple genetic alterations in a functionally coordinated manner. With this notion in mind, global mRNA expression analyses have been conducted to identify the gene network associated with early intrahepatic recurrence. (14)(15)(16)(17) Such molecular pathogenesis studies may lead to the development of gene-based biomarkers and novel therapeutic strategies. However, mRNA expression reflects the protein expression of only a small proportion of genes, probably because of post-translational control of protein quantity. (18)(19)(20)(21) To complement gene expression studies, global protein expression profiling, namely a proteomic approach, has been carried out for the study of HCC.(22-28) Conceptually, proteomics reflects the molecular background of cancer phenotypes more directly, as the final product of genetic and epigenetic events is the proteins contained in the cells.In this study we investigated the protein expression of HCC tissues from patients with early intrahepatic recurrence and from patients without recurrence. We used two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) to generate quantitative protein expression profiles, and data-mining methods...

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Section: Discussioncontrasting

confidence: 51%

Protein expression associated with early intrahepatic recurrence of hepatocellular carcinoma after curative surgery

et al. 2007

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“…In addition to snoRNAs, the upregulation of R2TP has also been reported in various cancer cells as shown in Table 2. For example, in human hepatocellular carcinoma (HCC), the mRNA and/or protein levels of RuvBL1/ pontin and RuvBL2/reptin are increased as compared to non-tumor liver (55)(56)(57)(58)(59)(60). Also, it has been shown that RuvBL1/pontin is overexpressed in breast, colon, and colorectal cancers (61)(62)(63)(64) and RuvBL2/reptin is overexpressed in breast, colon, gastric, and renal cell cancers (62,(65)(66)(67).…”

Section: Potential Role Of R2tp In Tumorigenesis Through Snornp Pathwaymentioning

confidence: 99%

The R2TP chaperone complex: its involvement in snoRNP assembly and tumorigenesis

Kakihara

Saeki

2014

Biomolecular Concepts

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R2TP was originally identified in yeast Saccharomyces cerevisiae as Hsp90 interacting complex, and is composed of four different proteins: Rvb1, Rvb2, Tah1, and Pih1. This complex is well-conserved in eukaryotes, and is involved in many cellular processes such as snoRNP biogenesis, RNA polymerase assembly, PIKK signaling, and apoptosis. An increasing number of research related to R2TP suggests a linkage of its function with tumorigenesis. In this review, we provide an overview of several recent studies on R2TP that are related to cell proliferation and carcinogenesis, and propose a possible role of R2TP in tumorigenesis through regulating snoRNA/snoRNP biogenesis.

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“…2-DE was performed according to the methods described previously. 7 We repeated each 2-DE experiment four times to confirm the spot patterns before proceeding with further analysis. Matching accuracy was 50%.…”

Section: Two-dimensional Gel Electrophoresis (2-de)mentioning

confidence: 99%

Influence of reproductive tract obstruction on expression of epididymal proteins and their restoration after patency

Wang²,

Liu³

et al. 2012

Asian J Androl

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Vasectomy is a simple and reliable method of male contraception. A growing number of men after vasectomy request vasectomy reversal due to various reasons. The pregnancy rate is lower than the patency rate after vasovasostomy and the pregnancy rate is time dependent. In this study, we evaluated the influence of reproductive tract obstruction on expression of epididymal proteins and their restoration after patency. Adult male Wistar rats were studied 30, 60 and 120 days after vasectomy, 30 days after vasovasostomy or after sham operations. Two-dimensional gel electrophoresis, mass-spectrometric technique, multidatabase search, Western blotting and real-time PCR were used to analyze the expression regulation of epididymal proteins. Total integrated intensity and total spot area of autoradiograms showed a consistent downward trend with time after obstruction, and this trend remained after patency. The intensity of the autoradiographic spots in three patency groups showed three trends: a downward trend, similar intensity and an upward trend compared with the correspondent obstruction group, respectively. Further verified experiments on human epididymis 2 (HE2), fertilization antigen-1 (FA-1), clusterin and PH20 demonstrated that compared with the correspondent obstruction group, the translation levels of HE2 and the mRNA transcription levels of HE2 showed an upward trend in patency groups, especially in the groups of obstruction for 60 days where the expression levels of HE2 were significantly upregulated after patency (P,0.05). Reproductive tract obstruction provokes a disregulation of gene expression in the epididymis and this disregulation remained after patency. Successful reversal may recover some proteins and the recovery is time dependent. Obstruction differentially alters mRNA transcription of different proteins and the content of proteins seemed to be easier to be influenced than the gene transcription. Vasovasostomy by modern microsurgical techniques remains the standard technique for vasectomy reversal with which all other methods of vasectomy reversal are compared. If success is defined that sperm can be detected in ejaculate following reversal, patency is over 80% in most microsurgical series, with patency approaching 100% in some reports. 1 However, pregnancies are obtained without assisted reproduction in 30%-75% of couples. 2 In other words, about 25% of couples are affected. Male factors account for about 50% of couples with infertility. 3 Approximately 10%-15% of infertile men suffer from azoospermia-complete absence of sperms in the ejaculate. Among these azoospermic patients, approximately 40% have complete obstruction in the ductal system and hence suffer from obstructive azoospermia. 4 Vasoepididymostomy is performed for congenital, infectious, postvasectomy or idiopathic epididymal obstruction. Following this type of microsurgery, 20%-40% of couples achieve pregnancy through intercourse without the assistance of reproductive techniques. 2 Similar to vasectomy reversal, the pregnancy rate is mu...

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Proteomic analysis of hepatitis B virus‐associated hepatocellular carcinoma: Identification of potential tumor markers

Cited by 105 publications

References 54 publications

Protein expression associated with early intrahepatic recurrence of hepatocellular carcinoma after curative surgery

Protein expression associated with early intrahepatic recurrence of hepatocellular carcinoma after curative surgery

The R2TP chaperone complex: its involvement in snoRNP assembly and tumorigenesis

Influence of reproductive tract obstruction on expression of epididymal proteins and their restoration after patency

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