Proteomic Analysis of Differentially Expressed Proteins in Resistant Soybean Leaves after Phakopsora pachyrhizi Infection

Abstract

Soybean rust caused by Phakopsora pachyrhizi is a destructive foliar disease in nearly all soybean‐producing countries. Understanding the host responses at the molecular level is certainly essential for effective control of the disease. To identify proteins involved in the resistance to soybean rust, differential proteomic analysis was conducted in soybean leaves of a resistant genotype after P. pachyrhizi infection. A total of 41 protein spots exhibiting a fold change >1.5 between the non‐inoculated and P. pachyrhizi‐inoculated soybean leaves at 12 and 24 h postinoculation (hpi) were unambiguously identified and functionally grouped into seven categories. Twenty proteins were up‐regulated and four proteins were down‐regulated at 12 hpi, whereas 18 proteins were up‐regulated and eight proteins were down‐regulated at 24 hpi. Generally, proteins involved in photosynthesis were down‐regulated, whereas proteins associated with disease and defense response, protein folding and assembly, carbohydrate metabolism and energy production were up‐regulated. Results are discussed in terms of the functional implications of the proteins identified, with special emphasis on their putative roles in defense. Abundance changes of these proteins, together with their putative functions reveal a comprehensive picture of the host response in rust‐resistant soybean leaves and provide a useful platform for better understanding of the molecular basis of soybean rust resistance.

Proteomic Analysis of Soybean Leaves in a Compatible and an Incompatible Interaction with Phakopsora Pachyrhizi

“…Although molecular studies are being conducted on this pathosystem, there is still little information on the molecular basis of this interaction [21,[24][25][26]. To develop rust-resistant soybean cultivars, a better understanding of the molecular basis and essential genes involved in defense responses is a key to control this disastrous disease [21]. As a result of this interaction, twelve differentially accumulated proteins were detected and identified in this study.…”

“…Understanding the host responses at the molecular level is certainly essential for effective control of the disease, and the molecular basis for rust resistance remained largely unknown in soybean [21]. The study of soybean genes that are differently expressed as a response to P. pachyrhizi can aid the identification of important genes involved in the resistance response and provide tools for genetic breeding programs for creation of soybean cultivars resistant to ASR [22,23].…”

“…The study of soybean genes that are differently expressed as a response to P. pachyrhizi can aid the identification of important genes involved in the resistance response and provide tools for genetic breeding programs for creation of soybean cultivars resistant to ASR [22,23]. Although molecular studies are being conducted on this pathosystem, there is still little information on the molecular basis of this interaction [21,[24][25][26]. To develop rust-resistant soybean cultivars, a better understanding of the molecular basis and essential genes involved in defense responses is a key to control this disastrous disease [21].…”

Proteomics Based Study of Soybean and Phakopsora pachyrhizi Interaction

“…In recent years, a few proteomic studies have examined the soybean proteome in response to ASR (6,22,35,40) and proteome of ASR (21,35). Based upon these studies, many genes have been identified that are possibly involved in the defense response to ASR.…”

“…Based upon these studies, many genes have been identified that are possibly involved in the defense response to ASR. Wang et al (40) used resistant soybean genotype to identify the differentially expressed proteins whereas Park et al (2013, unpublished) used two resistant and two susceptible soybean genotypes which are of different genetic background. In order to increase the chance of identifying true rust resistance associated proteins, we selected a pair of recombinant inbred lines that were segregating for rust resistance in the present study.…”