Proteomic Analysis of Soybean Leaves in a Compatible and an Incompatible Interaction with Phakopsora Pachyrhizi

Abstract

Asian soybean rust (ASR), which is incited by the fungus Phakopsora pachyrhizi, is considered one of the most aggressive diseases to the soybean culture. There are no commercial cultivars immune to the pathogen and the control measure currently used is the application of fungicides that harms the environment and increases production costs. For a better understanding of the host’s response to the pathogen at the molecular level, two soybean genotypes were analyzed (PI561356, resistant to ASR and Embrapa 48, susceptible) at 72 hours and 192 hours after inoculation with spores of P. pachyrhizi. Leaf protein profiles of the plants were compared by two-dimensional electrophoresis associated with mass spectrometry (MS). Twenty-two protein spots presented different levels when the two treatments were compared (inoculated vs. non-inoculated). From those, twelve proteins were identified by MS analysis. Some of them are involved in metabolic pathways related to plant defense against pathogens, as in the case of carbonic anhydrase, 1-deoxy-D-xylulose- 5-phosphate reductoisomerase, fructose-bisphosphate aldolase and glutamine synthetase. The possible biochemical-physiological meanings of our findings are discussed.

The number of measurements needed to obtain high reliability for traits related to enzymatic activities and photosynthetic compounds in soybean plants infected with Phakopsora pachyrhizi

“…The repeatability coefficient can be influenced by the genetic architecture of the trait and the environmental conditions under which the experiment is performed [ 15 ]; these factors make the study of repeatability coefficients important to estimate how many measurements should be done for each trait to guarantee a high accuracy. Several authors have investigated the changes related to enzymatic activities and photosynthetic compounds in soybean plants infected by Phakopsora pachyrhizi [ 17 – 20 ]. Although the biochemical and physiological changes caused by P .…”

Putative bacterial volatile-mediated growth in soybean (Glycine max L. Merrill) and expression of induced proteins under salt stress

“…In a proteomic study, GGH protein expression was induced in soybean root during early response to compatible symbiotic bacteria (Salavati et al 2012). Upregulation of GGH expression is also reported during induced systemic resistance in the presence of beneficial bacteria (Pereira et al 2014;Chen et al 2014).…”

“…Upregulation of GGH expression is also reported during induced systemic resistance in the presence of beneficial bacteria (Pereira et al . ; Chen et al . ).…”

Análise proteômica comparativa de cultivares de soja (Glycine max) suscetível e resistente à ferrugem asiática (Phakopsora pachyrhizi)

“…Esta enzima também foi detectada com abundância relativa aumentada em estudos realizados por Pereira et al (2013) , 1994).…”

“…Existem várias evidências que relatam a anidrase carbônica envolvida com mecanismos de defesa moleculares de plantas contra patógenos. Esses resultados, somados aos encontrados nos estudos realizados por Pereira et al (2013), indicam uma possível função da anidrase carbônica nos mecanismos metabólicos envolvidos na defesa da planta contra patógenos. A acumulação diferencial da anidrase carbónica, por exemplo, indica que a resistência sistêmica adquirida (SAR) foi ativada após a inoculação do patógeno na cultivar suscetível a P. Em condições de estresse abiótico (estresse por alagamento), esta enzima foi observada em raiz, hipocótilo e folhas de soja, sendo que em raiz e hipocótilo esta enzima apresentou uma redução em sua abundância relativa (KHATOON et al, 2012).…”