Proteomic analysis of differentially expressed proteins in Penaeus vannamei hemocytes upon Taura syndrome virus infection

Abstract

To understand molecular responses of crustacean hemocytes to virus infection, we applied 2-DE proteomics approach to investigate altered proteins in hemocytes of Penaeus vannamei during Taura syndrome virus (TSV) infection. At 24 h postinfection, quantitative intensity analysis and nano-LC-ESI-MS/MS revealed 11 forms of 8 proteins that were significantly up-regulated, whereas 9 forms of 5 proteins were significantly down-regulated in the infected shrimps. These altered proteins play important roles in host defense (hemocyanin, catalase, carboxylesterase, transglutaminase, and glutathione transferase), signal transduction (14-3-3 zeta), carbohydrate metabolism (acetylglucosamine pyrophosphorylase), cellular structure and integrity (beta-tubulin, beta-actin, tropomyosin, and myosin), and ER-stress response (protein disulfide isomerase). Semiquantitative RT-PCR and Western blot analysis confirmed the upregulation of 14-3-3 at both mRNA and protein levels. Interestingly, several altered protein spots were identified as fragments of hemocyanin. Mass spectrometric analysis showed that the hemocyanin spots at acidic and basic regions represented the C- and N-terminal hemocyanin fragments, respectively. As three-quarters of C-terminal fragments were up-regulated, whereas two-thirds of N-terminal hemocyanin fragments were down-regulated, we therefore hypothesize that C- and N-terminal hemocyanin fragments may have differential roles in hemocytes. Further investigation of these data may lead to better understanding of the molecular responses of crustacean hemocytes to TSV infection.

Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus

“…Ferritin was also up-regulated in WSSV-resistant shrimp and in WSSV-challenged shrimp (2, 7). Carboxylesterase was also up-regulated at the protein level in taura syndrome virus-challenged shrimp (8). In the present study, induction of arginase and ribosomal protein mRNAs was also observed in this cluster during viral infection.…”

The proteomic response of cheliped myofibril tissue in the eurythermal porcelain crabPetrolisthes cinctipesto heat shock following acclimation to daily temperature fluctuations

“…In contrast, all six isoforms of cluster II had masses between 24 and 48 kDa, and higher pIs than isoforms from cluster I. An almost identical pattern of hemocyanin isoforms was observed in hemocytes of the Pacific white shrimp Penaeus vannamei upon virus infection (Chongsatja et al, 2007). Subsequent work showed that isoforms similar to those with low molecular mass in cluster II are peptides cleaved off the N-terminal hemocyanin sequence in white shrimp (Havanapan et al, 2009).…”

El camarón como una fuente de alérgenos

“…Su contenido alergénico puede alterarse por diversos factores, como infección por virus, estrés por hipoxia y dieta en los cultivos de camarón (20)(21)(22)(23). En hemocitos del camarón L. vannamei se identificaron varios grupos de proteínas cuya expresión es regulada por la infección con el virus del síndrome de Taura (Taura Syndrome Virus, TSV) (24). Algunas de las proteínas alteradas participan en la respuesta inmunitaria del camarón, en transducción de señales, en el metabolismo de carbohidratos y en estrés celular; entre estas, se identificaron los alérgenos conocidos, Figura 1.…”