Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus

A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens

“…The majority of the upregulated genes at the early stage of Vibrio infection might be important in the immune [6,8,15]. Since P. monodon shrimps at 48 hpi of virus were moribund, the host machinery at this stage was probably taken over by the virus for the production of more virus particles.…”

“…One mg of total RNA was reverse transcribed into cDNA using the ImPromp II Reverse Transcription System (Promega, USA) according to the manufacturer's instructions. The real-time PCR was performed by an iCycler iQTM Real-Time Detection system using iQ SYBR Green Supermix (Bio-Rad, USA) as described by Pongsomboon et al [8]. The primers and annealing temperatures of the six genes selected to check the microarray expression results are shown in the Table 1.…”

“…Each EST clone was spotted in duplicate. Microarray hybridization and analysis were performed as described by Pongsomboon et al [8]. Briefly, 10 mg of total RNA from 10 individual shrimps was reverse-transcribed and the synthesized cDNA obtained was labeled with Cy3 or Cy5 using a Labelstar Array kit (QIAGEN, Germany).…”

Identification and expression analysis of nascent polypeptide-associated complex alpha gene in response to immune challenges in Japanese flounder Paralichthys olivaceus

“…Consistent with these speculations, NACA has been found to be one of the differentially expressed genes in HIV-1-infected human CEM cells [28]. It has also been demonstrated that NACA expression was up-regulated in Chinook salmon embryonic cells persistently infected with infectious pancreatic necrosis virus [29] and in Penaeus monodon hemocytes following infection with yellow head virus [30].…”

Seasonal and spatial influences on gene expression in Antarctic krill Euphausia superba

“…Most of what is known in terms of immune function in crusta ceans derives from studies of diseases of commercially-reared shrimps such as Penaeus monodon and Fenneropenaeus chinensis. Pongsomboon et al (2008b) found that shrimps up-regulated both C-type lectin and cathepsin L-like cysteine peptidases when infected with yellow head virus. A similar response was observed upon infection with white spot syndrome virus (WSSV) (Aoki et al 2011).…”