Proteomic analysis of common bean seed with storage protein deficiency reveals up-regulation of sulfur-rich proteins and starch and raffinose metabolic enzymes, and down-regulation of the secretory pathway

Marsolais, Frédéric; Pająk, Agnieszka; Yin, Fuqiang; Taylor, Meghan; Gabriel, Michelle; Merino, Diana M.; Vanessa, Ma.; Kameka, Alexander; Vijayan, Perumal; Pham, Hai; Huang, Shangzhi; Rivoal, Jean; Bett, Kirstin E.; Hernández-Sebastià, Cinta; Liu, Qiang; Bertrand, Annick; Chapman, Ralph

doi:10.1016/j.jprot.2010.03.013

Cited by 61 publications

(69 citation statements)

References 57 publications

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“…Common bean ( Phaseolus vulgaris L.) accumulates large amounts of a γ-glutamyl dipeptide of S-methyl-cysteine, a non-protein amino acid (Taylor et al, 2008). Interestingly, the removal of 7S globulin and phytohemagglutinin in Phaseolus vulgaris enhanced the accumulation of sulfur-rich proteins (Marsolais et al, 2010). Additionally, an increase in cysteine and methionine was reported which occurred at the expense of S-methylcysteine.…”

Section: Discussionmentioning

confidence: 99%

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

2014

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Expression of heterologous methionine-rich proteins to increase the overall sulfur amino acid content of soybean seeds has been only marginally successful, presumably due to low accumulation of transgenes in soybeans or due to gene silencing. Proteome rebalancing of seed proteins has been shown to promote the accumulation of foreign proteins. In this study, we have utilized RNAi technology to suppress the expression of the β-conglycinin, the abundant 7S seed storage proteins of soybean. Western blot and 2D-gel analysis revealed that β-conglycinin knockdown line (SAM) failed to accumulate the α′, α, and β-subunits of β-conglycinin. The proteome rebalanced SAM retained the overall protein and oil content similar to that of wild-type soybean. We also generated transgenic soybean lines expressing methionine-rich 11 kDa δ-zein under the control of either the glycinin or β-conglycinin promoter. The introgression of the 11 kDa δ-zein into β-conglycinin knockdown line did not enhance the accumulation of the 11 kDa δ-zein. However, when the same plants were grown in sulfur-rich medium, we observed 3- to 16-fold increased accumulation of the 11 kDa δ-zein. Transmission electron microscopy observation revealed that seeds grown in sulfur-rich medium contained numerous endoplasmic reticulum derived protein bodies. Our findings suggest that sulfur availability, not proteome rebalancing, is needed for high-level accumulation of heterologous methionine-rich proteins in soybean seeds.

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Section: Discussionmentioning

confidence: 99%

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

2014

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“…A similar group of proteins has been found to be up-regulated in the common bean (Phaseolus vulgaris) in response to storage protein deficiency [43]. AGPSU1 has been previously shown to play a crucial role in carbon accumulation directed to starch formation in preference to the synthesis of triacylglycerol [44].…”

Section: 3mentioning

confidence: 98%

Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri

Bihan

Martin

Chirnside

et al. 2011

Journal of Proteomics

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“…It is becoming increasingly popular because of its health benefits (Campos-Vega, Loarca-Piña, & Oomah, 2010), and it is also an important model for research on seed storage proteins (Marsolais et al, 2010). Globulins (65% w/w) are the major protein components in beans, followed by albumins, glutelins, and prolamins (15, 10, and 2% w/w, respectively) (Montoya, Lallès, Beebe, & Leterme, 2010).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and metal chelating activities of peptide fractions from phaseolin and bean protein hydrolysates

et al. 2012

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Proteomic analysis of common bean seed with storage protein deficiency reveals up-regulation of sulfur-rich proteins and starch and raffinose metabolic enzymes, and down-regulation of the secretory pathway

Cited by 61 publications

References 57 publications

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri

Antioxidant and metal chelating activities of peptide fractions from phaseolin and bean protein hydrolysates

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