Proteomic analysis of bacterial (outer) membrane vesicles: progress and clinical potential

Kroniger, Tobias; Otto, Andreas; Becher, Dörte

doi:10.1080/14789450.2018.1505509

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…The OMV-enriched proteome of two human microbiota, Bacteroides fragilis and Bacteroides thetaiotaomicron showed high glycosidases and proteases, including those active in vitro [ 36 ]. Moreover, proteomics has identified different putative biologically active molecules like nucleotides and immunogenic peptidoglycan [ 37 ]. OMV proteins are involved in communication, nutrient acquisition, stress responses, and virulence [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692

Maphosa

Moleleki

2021

Microorganisms

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Pectobacterium brasiliense (Pbr) 1692 is an aggressive phytopathogen affecting a broad host range of crops and ornamental plants, including potatoes. Previous research on animal pathogens, and a few plant pathogens, revealed that Outer Membrane Vesicles (OMVs) are part of Gram-negative bacteria’s (GNB) adaptive toolkit. For this reason, OMV production and subsequent release from bacteria is a conserved process. Therefore, we hypothesized that OMVs might transport proteins that play a critical role in causing soft rot disease and in the survival and fitness of Pbr1692. Here, we show that the potato pathogen, Pbr1692, releases OMVs of various morphologies in Luria Bertani media at 31 °C. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) confirmed the production of OMVs by Pbr1692 cells. Transmission Electron Microscopy showed that these exist as chain-, single-, and double-membrane morphologies. Mass spectrometry followed by Gene Ontology, Clusters of Orthologous Groups, Virulence Factor, CAZymes, Antibiotic Resistance Ontology, and Bastion6 T6SE annotations identified 129 OMV-associated proteins with diverse annotated roles, including antibiotic stress response, virulence, and competition. Pbr1692 OMVs contributed to virulence in potato tubers and elicited a hypersensitive response in Nicotiana benthamiana leaves. Furthermore, Pbr1692 OMVs demonstrated antibacterial activity against Dickeya dadantii.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692

Maphosa

Moleleki

2021

Microorganisms

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“…Most of them are adhesins and fitness factors that help bacterial growth and survival in the harmful gut environment (Aguilera et al., 2014 ; Hong et al., 2019 ). Reviews with specific information on proteomic analyses of BEVs are available (Kroniger et al., 2018 ; Lee et al., 2016 ). Regarding BEVs from Gram‐positive bacteria, proteomic analyses in different species ( S. aureus , S. pyogenes , S. pneumoniae , and S. agalactiae, B. anthracis ) identified membrane and cytoplasmic proteins related with several biological processes.…”

Section: Bacterial Extracellular Membrane Vesiclesmentioning

confidence: 99%

Microbiota‐derived extracellular vesicles in interkingdom communication in the gut

Díaz-Garrido

Badı́a

Baldomà

2021

J of Extracellular Vesicle

148

112

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The intestine is fundamental in controlling human health. Intestinal epithelial and immune cells are continuously exposed to millions of microbes that greatly impact on intestinal epithelial barrier and immune function. This microbial community, known as gut microbiota, is now recognized as an important partner of the human being that actively contribute to essential functions of the intestine but also of distal organs. In the gut ecosystem, bidirectional microbiota‐host communication does not involve direct cell contacts. Both microbiota and host‐derived extracellular vesicles (EVs) are key players of such interkingdom crosstalk. There is now accumulating body of evidence that bacterial secreted vesicles mediate microbiota functions by transporting and delivering into host cells effector molecules that modulate host signalling pathways and cell processes. Consequently, vesicles released by the gut microbiota may have great influence on health and disease. Here we review current knowledge on microbiota EVs and specifically highlight their role in controlling host metabolism, intestinal barrier integrity and immune training.

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“…Recent progress in mass spectrometry-based high-throughput proteomic profiling sheds light on protein research of MVs. 34,35 By now, thousands of vesicular proteins have been identified in periodontal pathogens, and available proteome analysis results are presented in Table 1. Further, based on proteomic profiling data, corresponding predicted subcellular location of vesicular proteins in different bacterial species is summarized (Figure 2).…”

Section: Proteinsmentioning

confidence: 99%

Membrane vesicles from periodontal pathogens and their potential roles in periodontal disease and systemic illnesses

Cao

2021

J of Periodontal Research

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The oral cavity is a dynamic "open growth system" of microbiome, which contains over 700 species of bacteria. [1][2][3] Typically, periodontal disease status, disease progression, and unsuccessful therapy have long been ascribed to the dysbiosis between host and microbial communities. Periodontitis has a sophisticated microbial ecosystem that colonizes in the periodontal pockets, primarily represented by "the red complex," Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. 4 Emerging evidence has extended the pathogenic microbial spectrums with gram-negative Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum; 5,6 gram-positive Streptococcus spp. and Filifactor alocis; 7,8 and many others. These periodontopathogens possess abundant virulence factors, among which vesicles exfoliated from bacterial membranes are getting increasing attention.Membrane vesicles (MVs) are spherical nanostructures with size ranging between 20-400 nm, and they are secreted by diverse bacterial species, both gram-negative and gram-positive, pathogenic or nonpathogenic. 9,10 MVs enclose sorted cargo molecules and serve essential biological functions, such as toxin transport, immunomodulation, biofilm formation, gene transfer, and intercellular interactions. 9 The recent identification of outer membrane vesicles (OMVs), together with outer-inner membrane vesicles (OIMVs) and

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Proteomic analysis of bacterial (outer) membrane vesicles: progress and clinical potential

Cited by 6 publications

References 27 publications

Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692

Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692

Microbiota‐derived extracellular vesicles in interkingdom communication in the gut

Membrane vesicles from periodontal pathogens and their potential roles in periodontal disease and systemic illnesses

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