Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692

Maphosa, Silindile; Moleleki, Lucy N.

doi:10.3390/microorganisms9091918

Cited by 11 publications

(16 citation statements)

References 78 publications

(118 reference statements)

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“…Our results were similar to those previously reported for Pectobacterium, where crude MVs were obtained with different isolation methods and different growth media [29,30]. Several outer membrane markers were found, including Omp proteins, the major outer membrane lipoprotein Lpp, and elongation factors.…”

Section: Discussionsupporting

confidence: 91%

“…Many of the observed large (100-300 nm) vesicles in the TEM images were similar to O-IMVs. O-IMVs were also observed in [29,30], which may suggest that they are commonly produced by bacteria.…”

Section: Discussionmentioning

confidence: 88%

“…First characterisations of the MVs of Pectobacterium were published in 2020 and 2021 [29,30]. To date, the MVs production was reported for P. atrosepticum [34], P. betavasculorum [29], and P. brasiliense [30]. In our work, we characterise MVs of three other Pectobacterium species: P. zantedeschiae, P. ordoriferum and P. versatile.…”

Section: Discussionmentioning

confidence: 92%

“…MVs production in Pectobacterium was first reported in 1992 [34]. First characterisations of the MVs of Pectobacterium were published in 2020 and 2021 [29,30]. To date, the MVs production was reported for P. atrosepticum [34], P. betavasculorum [29], and P. brasiliense [30].…”

Section: Discussionmentioning

confidence: 99%

“…MVs also seem to be the alternate delivery route for extracellular enzymes excreted through T2SS and possibly type III (T3SS) secretion systems [25,26,28]. Recently, the protein content of Pectobacterium MVs has been described for P. betavasculorum [29] and P. brasiliense [30]. The analyses revealed a presence of several proteins involved in a host-pathogen interplay, including proteases, pectate lyases and oligoglycan transporter (KdgM).…”

Section: Introductionmentioning

confidence: 99%

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Membrane Vesicles of Pectobacterium as an Effective Protein Secretion System

Jońca

Waleron

Czaplewska

et al. 2021

IJMS

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Bacteria of genus Pectobacterium are Gram-negative rods of the family Pectobacteriaceae. They are the causative agent of soft rot diseases of crops and ornamental plants. However, their virulence mechanisms are not yet fully elucidated. Membrane vesicles (MVs) are universally released by bacteria and are believed to play an important role in the pathogenicity and survival of bacteria in the environment. Our study investigates the role of MVs in the virulence of Pectobacterium. The results indicate that the morphology and MVs production depend on growth medium composition. In polygalacturonic acid (PGA) supplemented media, Pectobacterium produces large MVs (100–300 nm) and small vesicles below 100 nm. Proteomic analyses revealed the presence of pectate degrading enzymes in the MVs. The pectate plate test and enzymatic assay proved that those enzymes are active and able to degrade pectates. What is more, the pathogenicity test indicated that the MVs derived from Pectobacterium were able to induce maceration of Zantedeschia sp. leaves. We also show that the MVs of β-lactamase producing strains were able to suppress ampicillin activity and permit the growth of susceptible bacteria. Those findings indicate that the MVs of Pectobacterium play an important role in host-pathogen interactions and niche competition with other bacteria. Our research also sheds some light on the mechanism of MVs production. We demonstrate that the MVs production in Pectobacterium strains, which overexpress a green fluorescence protein (GFP), is higher than in wild-type strains. Moreover, proteomic analysis revealed that the GFP was present in the MVs. Therefore, it is possible that protein sequestration into MVs might not be strictly limited to periplasmic proteins. Our research highlights the importance of MVs production as a mechanism of cargo delivery in Pectobacterium and an effective secretion system.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 88%