Proteomic analysis of a fraction enriched in cell envelope proteins of Acinetobacter baumannii

Martí, Sara; Sánchez‐Céspedes, Javier; Oliveira, Eliandre de; Bellido, David; Giralt, Ernest; Vilà, Jordi

doi:10.1002/pmic.200500323

Cited by 31 publications

(40 citation statements)

References 15 publications

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“…1C). Interestingly, of the 8 proteins predicted to be cytoplasmic proteins, 3 have previously been identified in outer membrane fractions of A. baumannii (20), and 2 are ribosomal subunits which have been shown to be associated with bacterial outer membranes (29). These results suggest that the OMCs consisted primarily of proteins from the bacterial outer membrane.…”

Section: Resultsmentioning

confidence: 51%

Vaccination with Outer Membrane Complexes Elicits Rapid Protective Immunity to Multidrug-Resistant Acinetobacter baumannii

et al. 2011

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Acinetobacter baumannii causes pneumonias, bacteremias, and skin and soft tissue infections, primarily in the hospitalized setting. The incidence of infections caused by A. baumannii has increased dramatically over the last 30 years, while at the same time the treatment of these infections has been complicated by the emergence of antibiotic-resistant strains. Despite these trends, no vaccines or antibody-based therapies have been developed for the prevention of A. baumannii infection. In this study, an outer membrane complex vaccine consisting of multiple surface antigens from the bacterial membrane of A. baumannii was developed and tested in a murine sepsis model. Immunization elicited humoral and cellular responses that were able to reduce postinfection bacterial loads, reduce postinfection proinflammatory cytokine levels in serum, and protect mice from infection with human clinical isolates of A. baumannii. A single administration of the vaccine was able to elicit protective immunity in as few as 6 days postimmunization. In addition, vaccine antiserum was used successfully to therapeutically rescue naïve mice with established infection. These results indicate that prophylactic vaccination and antibody-based therapies based on an outer membrane complex vaccine may be viable approaches to preventing the morbidity and mortality caused by this pathogen.

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Section: Resultsmentioning

confidence: 51%

Vaccination with Outer Membrane Complexes Elicits Rapid Protective Immunity to Multidrug-Resistant Acinetobacter baumannii

et al. 2011

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“…2-DE protein analyses were performed following the conditions described elsewhere. 29 The protein spot of interest was recovered, digested and sequenced by MALDI-TOF-TOF analysis.…”

Section: Methodsmentioning

confidence: 99%

Salicylate increases the expression of marA and reduces in vitro biofilm formation in uropathogenic Escherichia coli by decreasing type 1 fimbriae expression

Vilà

Soto

2012

Virulence

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Escherichia coli is one of the most frequent bacteria implicated in biofilm formation, which is a dynamic process whose first step consists in bacteria adhesion to surfaces through type 1 fimbriae. Salicylate induces a number of morphological and physiological alterations in bacteria including the activation of the transcriptional regulator MarA. In this report the effects of salicylate on biofilm formation and their relationship with MarA were studied. An inverse relationship was observed between in vitro biofilm formation and salicylate concentration added to the culture medium. Salicylate increases the expression of marA and decreases the expression of fimA and fimB genes in the wild-type strain. In addition, the fimA and fimB expression was decreased in a MarR mutant in which marA was also overexpressed. In conclusion, the expression of type 1 fimbriae in presence of salicylate may be regulated by the level of marA expression through fimB regulator, albeit through neither the ompX nor the tolC genes.

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“…are not responsible for altering OMV production or toxicity. Carbapenem resistant A. baumannii has been already studied using outer membrane proteomics between wild type and resistance strain [108,[140][141][142]. Membrane proteomics result also showed that A. baumannii displays a vigorous and adaptable metabolism [143,144].…”

Section: Significance Of Omv Proteomics In the Study Of Drug Resistanmentioning

confidence: 99%

Significances of OMV and Extracellular Vesicle Proteomics

Tiwari¹

2017

J Data Mining Genomics Proteomics

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Outer Membrane Vesicle (OMV) proteome has been involved into the pathogenesis of diseases and resistance of microorganisms against a number of antibiotics, mechanism of action of probiotics and host-pathogen interaction etc. We have enlightened the role played by extracellular vesicles proteomics in the pathogenesis of different diseases related to human. Isolation succeeded by purification of ample amount of OMV from biological samples, is one of the most important steps for further proteome related analysis. With the development of both labelled and label-free methods used in proteomics, significant progress has been made in previous years in membrane proteomics. Hence, it is important to review the biological significance of proteins found in the OMV fractions using membrane proteomics approach. We have also explained methods used for isolation, purification and quantification of OMV. In the present review, it can be concluded that proteomic study of outer membrane and extracellular vesicles has now gained priority for the detailed study of disease pathogenesis, drug resistance, vaccine development, cell signalling etc.

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Proteomic analysis of a fraction enriched in cell envelope proteins of Acinetobacter baumannii

Cited by 31 publications

References 15 publications

Vaccination with Outer Membrane Complexes Elicits Rapid Protective Immunity to Multidrug-Resistant Acinetobacter baumannii

Vaccination with Outer Membrane Complexes Elicits Rapid Protective Immunity to Multidrug-Resistant Acinetobacter baumannii

Salicylate increases the expression of marA and reduces in vitro biofilm formation in uropathogenic Escherichia coli by decreasing type 1 fimbriae expression

Significances of OMV and Extracellular Vesicle Proteomics

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