Significances of OMV and Extracellular Vesicle Proteomics

Abstract

Outer Membrane Vesicle (OMV) proteome has been involved into the pathogenesis of diseases and resistance of microorganisms against a number of antibiotics, mechanism of action of probiotics and host-pathogen interaction etc. We have enlightened the role played by extracellular vesicles proteomics in the pathogenesis of different diseases related to human. Isolation succeeded by purification of ample amount of OMV from biological samples, is one of the most important steps for further proteome related analysis. With the development of both labelled and label-free methods used in proteomics, significant progress has been made in previous years in membrane proteomics. Hence, it is important to review the biological significance of proteins found in the OMV fractions using membrane proteomics approach. We have also explained methods used for isolation, purification and quantification of OMV. In the present review, it can be concluded that proteomic study of outer membrane and extracellular vesicles has now gained priority for the detailed study of disease pathogenesis, drug resistance, vaccine development, cell signalling etc.

Streptomyces coelicolor Vesicles: Many Molecules To Be Delivered

“…Among the most interesting findings are Bfr, SapA, SodF1, cytochromes, and some Sec system proteins. Other studies have demonstrated that many of these proteins constitute the protein cargo of MVs in other organisms ( 19 , 57 – 59 ), although they have not detailed their sublocalization in vesicles, whether in the luminal or external region. Bfr and SodF1 are cytosolic proteins involved in the oxidative stress response ( 60 , 61 ).…”