Proteomic analyses revealed the antibacterial mechanism of Aronia melanocarpa isolated anthocyanins against Escherichia coli O157: H7

Deng, Haotian; Kong, Yanwen; Zhu, Jinyan; Jiao, Xinyao; Tong, Yuqi; Wan, Meizhi; Zhao, Yang; Lin, Sixu; Ma, Yan; Meng, Xianjun

doi:10.1016/j.crfs.2022.09.017

Cited by 4 publications

(7 citation statements)

References 47 publications

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“…The method of component identification of AMAs was described in the previous report. 22 In brief, the composition of AMAs was identified by liquid chromatography-mass spectrometry (HPLC-MS). It was identified that AMAs mainly contain four anthocyanins, namely cyanidin-3- O -galactoside, cyanidin-3- O -glucoside, cyanidin-3- O -arabinoside, and cyanidin-3- O -xyloside.…”

Section: Methodsmentioning

confidence: 99%

“…In the HPLC chromatogram, the peak area ratios of the four anthocyanins were 65.368%, 3.944%, 25.119% and 4.716% respectively. 22…”

Section: Preparation and Composition Identification Of Amasmentioning

confidence: 99%

“…The proteomics method was carried out according to the previous description. 21,22 In brief, E. coli O157:H7 cells were added with lysis buffer and centrifuged to obtain the supernatant. DTT was added to the sample, and iodoacetamide was added after a water bath at 56 °C for 1 h. 30 μg of the sample was added to the ultrafiltration tube, and the waste liquid was discarded after centrifugation.…”

Section: Proteomics Analysismentioning

confidence: 99%

“…Previous reports have shown that AMAs can enter Escherichia coli O157:H7 cells through the bacillary mechanosensitive channel and affect the expression of proteins involved in oxidative stress, protein biosynthesis, DNA replication and repair and other processes. 21,22 Furthermore, metabolomics and proteomics data were integrated. First, the pathway results were classified and analyzed, including metabolism, environmental information processing, cellular processes, human diseases, and genetic information processing.…”

Section: Integration Of Proteome and Metabolomementioning

confidence: 99%

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A multi-omics analysis strategy reveals the molecular mechanism of the inhibition of Escherichia coli O157:H7 by anthocyanins from Aronia melanocarpa and its application

Deng¹,

Jun²,

Li³

et al. 2023

Food Funct.

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Section: Methodsmentioning

confidence: 99%

“…In the HPLC chromatogram, the peak area ratios of the four anthocyanins were 65.368%, 3.944%, 25.119% and 4.716% respectively. 22…”

Section: Preparation and Composition Identification Of Amasmentioning

confidence: 99%

Section: Proteomics Analysismentioning

confidence: 99%

Section: Integration Of Proteome and Metabolomementioning

confidence: 99%

See 2 more Smart Citations

A multi-omics analysis strategy reveals the molecular mechanism of the inhibition of Escherichia coli O157:H7 by anthocyanins from Aronia melanocarpa and its application

Deng¹,

Jun²,

Li³

et al. 2023

Food Funct.

Self Cite

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show abstract

“…For example, the antimicrobial activity of epigallocatechin gallate from green tea against Streptococcus suis was analyzed by the synthesis of proteomics and TEM observation to infer a possible mechanism about cell wall and membrane damage [ 16 ]. In other research, the antibacterial mechanism of anthocyanins (isolated from Aronia melanocarpa ) against Escherichia coli was also investigated by the combination of proteomics and TEM observation to discover that oxidative damage to the cells occurred after treatment [ 17 ]. A few studies employed in silico target fishing combined with experimental verification by the surface plasmon resonance assay (SPR) in order to find potential targets of a known antibacterial chemical or to screen out potential novel drugs for a known antibacterial target [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Combining the In Silico and In Vitro Assays to Identify Strobilanthes cusia Kuntze Bioactives against Penicillin-Resistant Streptococcus pneumoniae

et al. 2023

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Leaves of Strobilanthes cusia Kuntze (S. cusia) are a widely used alexipharmic Traditional Chinese Medicine (TCM) in southern China for the prevention of cold and respiratory tract infectious diseases. One of the most common bacterial pathogens in the respiratory tract is the gram-positive bacterium Streptococcus pneumoniae. The antibiotic resistance of colonized S. pneumoniae makes it a more serious threat to public health. In this study, the leaves of S. cusia were found to perform antibacterial effects on the penicillin-resistant S. pneumoniae (PRSP). Confocal assay and Transmission Electron Microscopy (TEM) monitored the diminished cell wall integrity and capsule thickness of the PRSP with treatment. The following comparative proteomics analysis revealed that the glycometabolism-related pathways were enriched for the differentially expressed proteins between the samples with treatment and the control. To further delve into the specific single effective compound, the bio-active contents of leaves of S. cusia were analyzed by UPLC-UV-ESI-Q-TOF/MS, and 23 compounds were isolated for anti-PRSP screening. Among them, Tryptanthrin demonstrated the most promising effect, and it possibly inhibited the N-glycan degradation proteins, as suggested by reverse docking analysis in silico and further experimental verification by the surface plasmon resonance assay (SPR). Our study provided a research foundation for applications of the leaves of S. cusia as a TCM, and supplied a bio-active compound Tryptanthrin as a candidate drug skeleton for infectious diseases caused by the PRSP.

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Battling Salmonella enteritidis infections: integrating proteomics and in vivo assessment of Galla Chinensis tannic acid

Yan,

Zheng,

et al. 2024

BMC Vet Res

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Salmonella infections pose a significant threat to animal and human health. Phytochemicals present a potential alternative treatment. Galla chinensis tannic acid (GCTA), a hydrolyzable polyphenolic compound, inhibits bacterial growth and demonstrates potential as an alternative or supplement to antibiotics to prevent Salmonella infections. However, little is known about the antimicrobial mechanism of GCTA against Salmonella. Here, we revealed 456 differentially expressed proteins upon GCTA treatment, impacting pathways related to DNA replication, repair, genomic stability, cell wall biogenesis, and lipid metabolism using TMT-labeled proteomic analysis. TEM analysis suggested altered bacterial morphology and structure post-treatment. A Salmonella-infected-mouse model indicated that GCTA administration improved inflammatory markers, alleviated intestinal histopathological alterations, and reduced Salmonella enterica serovar Enteritidis (S. Enteritidis) colonization in the liver and spleen of Salmonella-infected mice. The LD50 of GCTA was 4100 mg/kg with an oral single dose, vastly exceeding the therapeutic dose. Thus, GCTA exhibited antibacterial and anti-infective activity against S. Enteritidis. Our results provided insight into the molecular mechanisms of these antibacterial effects, and highlights the potential of GCTA as an alternative to antibiotics.

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Proteomic analyses revealed the antibacterial mechanism of Aronia melanocarpa isolated anthocyanins against Escherichia coli O157: H7

Cited by 4 publications

References 47 publications

A multi-omics analysis strategy reveals the molecular mechanism of the inhibition of Escherichia coli O157:H7 by anthocyanins from Aronia melanocarpa and its application

A multi-omics analysis strategy reveals the molecular mechanism of the inhibition of Escherichia coli O157:H7 by anthocyanins from Aronia melanocarpa and its application

Combining the In Silico and In Vitro Assays to Identify Strobilanthes cusia Kuntze Bioactives against Penicillin-Resistant Streptococcus pneumoniae

Battling Salmonella enteritidis infections: integrating proteomics and in vivo assessment of Galla Chinensis tannic acid

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