Proteomic analyses of mitochondrial damage in postmortem beef muscles

Abstract

BACKGROUND The objective of the study was to examine the expression profiles of mitochondrial proteins in at‐death and 24 h postmortem (PM) using tandem mass tag (TMT) approach to characterize the mitochondria possible mechanisms that are affiliated with tenderization. RESULTS Results showed that the tender meat at 24 h PM emerged with more serious mitochondrial damage. Altogether 456 mitochondrial proteins were identified, including 442 down‐regulated and 14 up‐regulated proteins. These differentially‐expressed proteins were primarily involved in the progress of PM energy metabolism, apoptosis, and the morphological alterations of mitochondrial. Among them, 47 subunits (such as NDUFA2, COX4I1, and ATP5PB) were annotated into the oxidative phosphorylation pathway. VDAC1, VDAC2, and VDAC3 involving in the damage of MPTP, and IMMT, CHCHD3, APOL and APOO modulating the morphology of mitochondria, and DIABLO and AIFM1 released from mitochondria affect caspase's activation. HSPD1 and HSPE1 involved in apoptosis, mitochondrial physiological and morphological alterations. CONCLUSION The earlier‐mentioned proteins were validated as potential indicators of tenderness regulated by mitochondrial damage. These results highlighted that mitochondrial damage possibly participate in PM tenderization of beef muscles by energy metabolism and cell apoptosis status. © 2022 Society of Chemical Industry.

Differential Allele-Specific Expression Revealed Functional Variants and Candidate Genes Related to Meat Quality Traits in B. indicus Muscle

“…The release of cytochrome c induces apoptosis [ 51 , 53 ]. Apoptosis is a proteolytic process related to meat tenderization under oxidative stress [ 49 , 54 , 55 ], which can result from increased mitochondrial membrane permeability [ 50 ]. Utrophin, encoded by UTRN, the hub gene of M1, is a therapeutic target for Duchenne muscular disease [ 56 , 57 ].…”

Stunning methods before slaughter induce oxidation changes of large yellow croaker during cold storage: the role of mitochondria and underlying mechanisms

“…Mitochondria were extracted referring to the approach described by Liu et al 14 . with minor modification.…”

“…Mitochondria were extracted referring to the approach described by Liu et al 14 with minor modification. The back muscles of samples were used to extract mitochondria.…”

Morphological changes of mitochondria-related to apoptosis during postmortem aging of beef muscles

“…However, there are few studies on mitochondrial inner membrane cristae structure in PM skeletal muscles. Our previous reports have demonstrated that Mic60, Mic19, and Mic27, which regulate cristae morphology and the growth of mitochondrial contact sites, showed a considerable decrease in expression at 24 h PM ( Liu et al, 2022 ). However, how the inner mitochondria's morphological control and cristae production work is still unknown.…”