“…The primary organelle that produces reactive oxygen species (ROS) is the mitochondria, which is also the organelle that is most frequently targeted for attack ( Ding, Wei, Liu, Zhang, & Huang, 2022 ). ROS generated by single electron transfer could result in mitochondrial membrane swelling or loss of membrane potential after animal bleeding ( Liu et al, 2023 ; Zhang, Ma, & Kim, 2020 ). Cytochrome c is released from the mitochondria to the cytoplasm to bind Apaf-1, dATP/ATP and pro-caspase-9 when mitochondrial permeability increases, triggering executioner caspase activation ( Green, 2005 ).…”