Proteomic alteration of albumen by dietary vanadium in commercial egg-type layers

Abstract

Vanadium ( V ) is an ultratrace metal with the insulin-tropic properties and is often researched as the diabetes drug. However, in animals, V has been reported to have toxic effects on the development, immunity, oxidation–reduction equilibrium, gastrointestinal function, and so forth. Especially in poultry, supplementation of more than 10 mg of V/kg in the layer diets has been shown to adversely affect the egg production and egg quality. In this study, we supplemented 0 mg of V/kg, 5 mg of V/kg, and 10 mg of V/kg in the layer diets for 35 D and examined the quantitative proteomics of albumen for finding the possible target signaling pathway and mechanism of V action and made the preliminary verification. In contrast to the control group, V resulted in a significant drop in the albumen height, and in oviduct ampulla, the activity of total antioxidant capacity and glutathione peroxidase significantly decreased ( P = 0.01, P = 0.02), the content of malonic dialdehyde significantly increased ( P = 0.01), and the apoptosis rate significantly increased in the 5-mg V/kg and 10-mg V/kg treatment groups ( P ＜ 0.01). V affected 36 differentially accumulated proteins in albumen, with 23 proteins upregulated and 13 proteins downregulated. The expressions of innate protein albumen lysozyme (Q6LEL2), vitellogenin-2 (P02845), and the F1NWD0 protein in albumen belonged to the P53 family were significantly reduced, in contrast to the control ( P < 0.05), and the expression of riboflavin-binding protein (P02752) was significantly improved ( P < 0.05). The Hippo signaling pathway-fly, which is suitable for the key protein P53 as the most significantly affected network, might be important for discriminating V.

Natural Products of Plants and Animal Origin Improve Albumen Quality of Chicken Eggs

“…Further, diet-induced oxidative stress may degrade albumen proteins and invariably albumen quality. One study reported that vanadium-based diet caused a reduction in albumen quality by decreasing the abundance of an egg white protein; lysozyme (Q6LEL2) which possesses antimicrobial properties while increasing protease inhibitors that restrain activities of some proteins (87). It is crucial to enhance endogenous antioxidant enzymes in order to mitigate the adverse effects of oxidative stress on animal and its product as well.…”