Proteomes of the aphid Macrosiphum euphorbiae in its resistance and susceptibility responses to differently compatible parasitoids

Nguyen, Thi Thuy An; Boudreault, Simon; Michaud, Dominique; Cloutier, Conrad

doi:10.1016/j.ibmb.2008.04.005

Cited by 34 publications

(40 citation statements)

References 59 publications

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“…To our knowledge, only three studies have been published on the proteomics of host response to parasitization by parasitic wasps (Kaeslin et al, 2005;Song et al, 2008;Nguyen et al, 2008). The present study explored proteome changes in the plasma of an insect after parasitization by a parasitoid devoid of symbiotic viruses.…”

Section: Resultsmentioning

confidence: 99%

“…Perturbation in the expression of detoxification proteins has been found in insect subjected to an immune challenge, exposed to a temperature stress or to viral infections (de Morais Guedes et al, 2005;Lee et al, 2005). Using 2D gel analysis, Nguyen et al(2008) reported an increase in such types of proteins in the aphid Macrosiphum euphorbiae challenged by the parasitoids Aphidius nigripes or A. ervi. Altered expression of detoxification-related proteins following parasitization could be the result from the host response to the physiological injury brought about during the process of oviposition.…”

Section: Detoxification Proteinsmentioning

confidence: 99%

“…In addition, mRNA-based approaches displayed a poor correlation between the mRNA level and protein abundance, as such methods cannot either ensure that transcripts are actually translated, nor be used to analyze post-transcriptional fate of mRNA (Song et al, 2008). Recently, even several studies were involved in applying the powerful protein-based methods, proteomics, to investigate the host plasma changed by parasitoid (Kaeslin et al, 2005;Song et al, 2008;Nguyen et al, 2008); however, much further work is necessary. Moreover, in the investigations carried out at the gene or protein levels, the parasitoids were associated with PDVs or VLPs.…”

Section: Introductionmentioning

confidence: 99%

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Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Zhu

Yè

Fang

et al. 2009

J. Zhejiang Univ. Sci. B

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Abstract:Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects.

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Section: Resultsmentioning

confidence: 99%

Section: Detoxification Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Zhu

Yè

Fang

et al. 2009

J. Zhejiang Univ. Sci. B

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“…Actin is also the component of the cytoskeletal system that allows for the movement of cells and cellular processes. By using a proteomics approach, insect resistance to xenobiotics and pesticides, physical wound, parasitoid, and microorganism attack can readily cause a change in actin expression (Candas et al, 2003;Huang et al, 2004;Nguyen et al, 2008). Taken together, an increased expression of actin can reinforce a physical barrier to better resistance from further injury.…”

Section: Cytoskeleton Proteinsmentioning

confidence: 99%

“…Two-dimensional (2-DE) gel electrophoresis and mass spectrometry (MS) can provide powerful characterization of the proteins of interest. Recently, the change in protein variants in insects induced by infections, such as parasitoid attack, and chemical compound toxicity, has been investigated by using the 2-DE method (Candas et al, 2003;Huang et al, 2004;Nguyen et al, 2008;Song et al, 2008). The identification of proteins in the oriental fruit fly at different periods after insecticide application will provide more useful information of biologically important molecules to explore trichlorphon toxicity.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera; Tephritidae) larvae

Jin

Ling²,

Lin³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-treatment with trichlorphon in two-dimensional gel electrophoresis through mass spectrometry and protein sequencing. We found functional proteins that are responsible for signal transduction (pkaap and dual specificity tyrosine-phosphorylation-regulated kinase), immunity (hemolectin), synthesis and decomposition (twinstar, cathepsin B, ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (3): 2608-2619 (2012) Resistance response to organophosphorus insecticides 2609 RE66325p), oxidative stress metabolism (glutathione S transferase and CG7320), energy metabolism (Act57B), and cytoskeleton formation (actin). These proteins appear to be involved in the resistance response to trichlorphon.

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PARASITIZATION BY SCLERODERMA GUANI INFLUENCES EXPRESSION OF SUPEROXIDE DISMUTASE GENES IN TENEBRIO MOLITOR

Zhu

Sang-zi

Stanley

et al. 2014

Arch Insect Biochem Physiol

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Superoxide dismutase (SOD) is an antioxidant enzyme involved in detoxifying reactive oxygen species. In this study, we identified genes encoding the extracellular and intracellular copper-zinc SODs (ecCuZnSOD and icCuZnSOD) and a manganese SOD (MnSOD) in the yellow mealworm beetle, Tenebrio molitor. The cDNAs for ecCuZnSOD, icCuZnSOD, and MnSOD, respectively, encode 24.55, 15.81, and 23.14 kDa polypeptides, which possess structural features typical of other insect SODs. They showed 20-94% identity to other known SOD sequences from Bombyx mori, Musca domestica, Nasonia vitripennis, Pediculus humanus corporis, and Tribolium castaneum. Expression of these genes was analyzed in selected tissues and developmental stages, and following exposure to Escherichia coli and parasitization by Scleroderma guani. We recorded expression of all three SODs in cuticle, fat body, and hemocytes and in the major developmental stages. Relatively higher expressions were detected in late-instar larvae and pupae, compared to other developmental stages. Transcriptional levels were upregulated following bacterial infection. Analysis of pupae parasitized by S. guani revealed that expression of T. molitor SOD genes was significantly induced following parasitization. We infer that these genes act in immune response and in host-parasitoid interactions.

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Proteomes of the aphid Macrosiphum euphorbiae in its resistance and susceptibility responses to differently compatible parasitoids

Cited by 34 publications

References 59 publications

Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Characteristics of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera; Tephritidae) larvae

PARASITIZATION BY SCLERODERMA GUANI INFLUENCES EXPRESSION OF SUPEROXIDE DISMUTASE GENES IN TENEBRIO MOLITOR

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