Characteristics of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera; Tephritidae) larvae

Abstract

ABSTRACT. Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-treatment with trichlorphon in two-dimensional gel electrophoresis through mass spectrometry and protein sequencing. We found functional proteins that are responsible for signal transduction (pkaap and dual specificity tyrosine-phosphorylation-regulated kinase), immunity (hemolectin), synthesis and decomposition (twinstar, cathepsin B, ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (3): 2608-2619 (2012) Resistance response to organophosphorus insecticides 2609 RE66325p), oxidative stress metabolism (glutathione S transferase and CG7320), energy metabolism (Act57B), and cytoskeleton formation (actin). These proteins appear to be involved in the resistance response to trichlorphon.

Comparative proteomic analysis of Bactrocera dorsalis (Hendel) in response to thermal stress

“…approaches, 2-DE becomes a powerful tool to analyze protein responses. Such approaches have been used previously to identify the proteins involved in the response of Spodoptera litura to chemical toxicity (Huang et al, 2004;Jin et al, 2012) and the response of Macrosiphum euphorbiae to parasitoid challenge (Nguyen et al, 2008). Furthermore, the proteomic profiles of silk glands from silkworms fed on different diets and exposed to high-temperature treatment have also been analyzed using 2-DE (Li et al, 2012;Zhang et al, 2006).…”

Gut symbiont enhances insecticide resistance in a significant pest, the oriental fruit fly Bactrocera dorsalis (Hendel)

“…Several studies have been performed to elucidate the mechanism of B. dorsalis resistance to trichlorphon [ 33 , 34 ]. The functional proteins involved in the resistance response to trichlorphon were identified based on proteomic analyses of B. dorsalis treated with trichlorphon [ 34 ]. Independent from this, some pesticide-degrading bacteria have previously been isolated from trichlorphon-contaminated soil [ 35 ].…”