2010
DOI: 10.1086/655780
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Proteome‐Wide Anti–Hepatitis C Virus (HCV) and Anti‐HIV Antibody Profiling for Predicting and Monitoring the Response to HCV Therapy in HIV‐Coinfected Patients

Abstract: We quantified antibody responses to the HCV proteome that are associated with sustained virologic response (SVR) in HIV/HCV co-infected patients treated with pegylated interferon and ribavirin. Analysis of pre- and post-treatment samples revealed significant decreases in the combined anti-core, anti-E1 and anti-NS4 HCV antibody titers in those with SVR, but not in the relapsers or non-responders. Furthermore, anti-p24 HIV antibody titers inversely correlated with treatment response. These results suggest that … Show more

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Cited by 19 publications
(25 citation statements)
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References 15 publications
(18 reference statements)
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“…We chose to use serology for its advantages of tolerance for sequence divergence, a capacity to detect resolved as well as acute or active infections, and high sample throughput. We expected that like other RNA viruses, including HCV, different CHV variants would be genetically diverse, and we therefore synthesized antigen from the helicase domain of NS3, the most conserved viral protein in the genome and one that shows documented antigenicity in HCV (11).…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…We chose to use serology for its advantages of tolerance for sequence divergence, a capacity to detect resolved as well as acute or active infections, and high sample throughput. We expected that like other RNA viruses, including HCV, different CHV variants would be genetically diverse, and we therefore synthesized antigen from the helicase domain of NS3, the most conserved viral protein in the genome and one that shows documented antigenicity in HCV (11).…”
Section: Discussionmentioning
confidence: 99%
“…1B). To examine possible antigenic cross-reactivity, we tested the equine seropositive and seronegative samples against helicase protein of HCV (11) and found all these samples to be nonreactive (Fig. 1C).…”
Section: Use Of the Lips To Search Chv-related Viruses In Other Animalmentioning
confidence: 99%
See 1 more Smart Citation
“…The wide dynamic range of antibody detection for many of these LIPS tests is advantageous for monitoring response to treatment. LIPS profiling of antibodies generated against multiple hepatitis C virus (HCV) proteins has lead to the identification of biomarkers for differentiating long-term responders for HCV treatment in HCV-HIV-coinfected individuals [12]. In these studies the combined antibody titers to three HCV proteins, core, ENV1 and NS4, had potential for identification of long term responders from relapsers at 40 weeks following treatment with interferon-α and ribavirin for HCV infection.…”
Section: Infectious Disease Diagnostics and Antigen Discovery By Lipsmentioning
confidence: 99%
“…To date, several LIPS formats are available including single tube assays to measure one antigen in a single sample (Burbelo et al 2005), iterative testing using microtiter plates (Burbelo et al 2010b; Burbelo et al 2010c; Burbelo et al 2009d) and microwell arrays for simultaneously profiling antibody responses to panels of antigens (Burbelo et al 2011). In addition, a rapid format called QLIPS uses a relatively short incubation of 10 minutes but still shows sensitivity and specificity comparable to the standard 2 hour LIPS format (Burbelo et al 2009a; Burbelo et al 2009c; Burbelo et al 2008).…”
Section: Introductionmentioning
confidence: 99%