Proteome profile and biological activity of caprine, bovine and human milk fat globules

Spertino, Stefano; Cipriani, Valentina; Angelis, Chiara De; Giuffrida, Maria Gabriella; Marsano, Francesco; Cavaletto, Maria

doi:10.1039/c2mb05400k

Cited by 47 publications

(33 citation statements)

References 48 publications

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“…These proteins are thus specific to EV derived from milk (and have not been identified in other EV preparations). Moreover, OLAH could potentially be used to differentiate between milk-derived EV and MFG, as some studies performed on MFG have reported the presence of PTHLH, whereas OLAH has never been detected in this milk component (31)(32)(33)37). Both OLAH and PTHLH have been detected in whey (36,45), which contains EV but not MFG, further supporting the idea that milk-derived EV express both OLAH and PTHLH.…”

Section: Discussionsupporting

confidence: 53%

Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components

Herwijnen

Zonneveld

Goerdayal³

et al. 2016

Molecular & Cellular Proteomics

135

158

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Section: Discussionsupporting

confidence: 53%

Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components

Herwijnen

Zonneveld

Goerdayal³

et al. 2016

Molecular & Cellular Proteomics

135

158

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“…A primary function of PLIN proteins is the regulation of lipolysis by controlling lipase access to the TAG core of CLD (33, 37–39). Perilipin 2, which appears to be the primary PLIN family member expressed by mammary epithelial cells (16, 40–42), is an abundant protein on lipid droplets isolated from the milk of rodents, cattle, and humans (16, 43, 44). PLIN2 transcripts are selectively enriched in mammary epithelial cells in the mouse mammary gland relative to other cell types (42), and their levels undergo about a 30-fold increase during mammary gland differentiation (42).…”

Section: Mechanisms Of Milk Lipid Productionmentioning

confidence: 99%

Milk lipid secretion: recent biomolecular aspects

McManaman

2012

BioMolecular Concepts

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Neonates of most species depend on milk lipids for calories, fat-soluble vitamins, and bioactive lipid components for growth and development during the postnatal period. To meet neonatal nutrition and development needs, the mammary gland has evolved efficient mechanisms for synthesizing and secreting large quantities of lipid during lactation. Although the biochemical steps involved in milk lipid synthesis are understood, the identities of the genes mediating these steps and the molecular physiology of milk lipid production and secretion have only recently begun to be understood in detail through advances in mouse genetics, gene expression analysis, protein structural properties, and the cell biology of lipid metabolism. This review discusses emerging data about the molecular, cellular, and structural determinants of milk lipid synthesis and secretion within the context of physiological functions.

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“…; Spertino et al . ). To our knowledge, this is the first use of proteomics technology to discern changes in secretory mechanisms following knockout of a specific mediator of apocrine secretion.…”

Section: Introductionmentioning

confidence: 97%

Xanthine oxidoreductase mediates membrane docking of milk‐fat droplets but is not essential for apocrine lipid secretion

Monks

Dzieciątkowska

Bales

et al. 2016

The Journal of Physiology

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Apocrine secretion is utilized by epithelial cells of exocrine glands. These cells bud off membrane-bound particles into the lumen of the gland, losing a portion of the cytoplasm in the secretion product. The lactating mammary gland secretes milk lipid by this mechanism, and xanthine oxidoreductase (XOR) has long been thought to be functionally important. We generated mammary-specific XOR knockout (MGKO) mice, expecting lactation to fail. Histology of the knockout glands showed very large lipid droplets enclosed in the mammary alveolar cells, but milk analysis showed that these large globules were secreted. Butyrophilin, a membrane protein known to bind to XOR, was clustered at the point of contact of the cytoplasmic lipid droplet with the apical plasma membrane, in the wild-type gland but not in the knockout, suggesting that XOR mediates 'docking' to this membrane. Secreted milk fat globules were isolated from mouse milk of wild-type and XOR MGKO dams, and subjected to LC-MS/MS for analysis of protein component. Proteomic results showed that loss of XOR leads to an increase in cytoplasmic, cytoskeletal, Golgi apparatus and lipid metabolism proteins associated with the secreted milk fat globule. Association of XOR with the lipid droplet results in membrane docking and more efficient retention of cytoplasmic components by the secretory cell. Loss of XOR then results in a reversion to a more rudimentary, less efficient, apocrine secretion mechanism, but does not prevent milk fat globule secretion.

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Proteome profile and biological activity of caprine, bovine and human milk fat globules

Cited by 47 publications

References 48 publications

Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components

Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components

Milk lipid secretion: recent biomolecular aspects

Xanthine oxidoreductase mediates membrane docking of milk‐fat droplets but is not essential for apocrine lipid secretion

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