Milk lipid secretion: recent biomolecular aspects

McManaman, James L.

doi:10.1515/bmc-2012-0025

Cited by 34 publications

(34 citation statements)

References 111 publications

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“…). All milk protein and half of the total fatty acids in bovine milk are synthesized within the mammary epithelial cells (Mcmanaman ), and milk protein and lipid biosynthesis are regulated at many levels within the BMEC. In our data, about 95 proteins related to lipid metabolism were identified with COG categories.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies demonstrated that the biochemical pathways in mammary glands related to biosynthesis and secretion of lipids and proteins are regulated by complex gene networks (Bionaz & Loor 2008;Liu et al 2013). All milk protein and half of the total fatty acids in bovine milk are synthesized within the mammary epithelial cells (Mcmanaman 2012), and milk protein and lipid biosynthesis are regulated at many levels within the BMEC. In our data, about 95 proteins related to lipid metabolism were identified with COG categories.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells

Wang

et al. 2017

Animal Science Journal

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Heat stress can play a negative effect on milk yield and composition of dairy cattle, leading to immeasurable economic loss. The basic components of the mammary gland are the alveoli; these alveolar mammary epithelial cells reflect the milk producing ability of dairy cows. In this study, we exposed bovine mammary epithelial cells to heat stress and compared them to a control group using isobaric tags for relative and absolute quantitation combined with liquid chromatography coupled with tandem mass spectrometry. Compared with a control group, 104 differentially elevated proteins (>1.3-fold) and 167 decreased proteins (<0.77-fold) were identified in the heat treatment group. Gene Ontology analysis identified a majority of the differentially expressed proteins are associated in cell-substrate junction assembly, catabolic processes and metabolic processes. Some of these significantly regulated proteins were related to the synthesis and secretion of milk, such as milk protein and fat. This finding was further supported by the results obtained from the reduced β-casein expression through the system of plasminogen activator - plasminogen - plasmin and decreased fatty acid synthase could partly explain why milk fat synthesis ability of dairy cows decreased under heat stress. Our results highlight the effects of heat stress on synthesis of milk protein and fat, thus providing additional clues for further studies of heat stress on dairy milk production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells

Wang

et al. 2017

Animal Science Journal

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“…Notably, it was observed that oxytocininduced myoepithelial cell contraction is required to release mature LDs from secretory cells into luminal spaces ( Figure 1D; Masedunskas et al, 2017). This suggests that LD droplet secretion is intermittently stimulated by milk let-down (Masedunskas et al, 2017), and is not a continuous process as previously assumed from static observations (Mather and Keenan, 1998;Neville, 2009;McManaman, 2012). Intriguingly, alveolar cells switch their cellular function from LD secretion to uptake during mammary gland involution, triggering a complex program of cell death that returns the mammary gland to a near pre-pregnant state (Kreuzaler et al, 2011;Sargeant et al, 2014).…”

Section: Multidimensional Imaging Of the Lactating Mammary Glandmentioning

confidence: 50%

“…Lipids, particularly triacylglycerols, are major milk constituents (Oftedal, 1984) that are packaged and secreted in the form of membrane-coated lipid droplets (LDs) during lactation (Walther et al, 2017). While classical biochemical and morphological analyses have revealed valuable insights into LD assembly, fusion and secretion, the kinetics underlying this dynamic process remained unclear (Mather and Keenan, 1998;McManaman, 2012).…”

Section: Multidimensional Imaging Of the Lactating Mammary Glandmentioning

confidence: 99%

Multidimensional Imaging of Mammary Gland Development: A Window Into Breast Form and Function

Lloyd-Lewis

2020

Front. Cell Dev. Biol.

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An in-depth appreciation of organ form and function relies on the ability to image intact tissues across multiple scales. Difficulties associated with imaging deep within organs, however, can preclude high-resolution multidimensional imaging of live and fixed tissues. This is particularly challenging in the mammary gland, where the epithelium lies deeply encased within a stromal matrix. Recent advances in deep-tissue and live imaging methodologies are increasingly facilitating the visualization of complex cellular structures within their native environment. Alongside, refinements in optical tissue clearing and immunostaining methods are enabling 3D fluorescence imaging of whole organs at unprecedented resolutions. Collectively, these methods are illuminating the dynamic biological processes underlying tissue morphogenesis, homeostasis, and disease. This review provides a snapshot of the current and state-of-the-art multidimensional imaging techniques applied to the postnatal mammary gland, illustrating how these approaches have revealed important new insights into mammary gland ductal development and lactation. Continual evolution of multidimensional image acquisition and analysis methods will undoubtedly offer further insights into mammary gland biology that promises to shed new light on the perturbations leading to breast cancer.

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“…1) [13], and en masse transport of phospholipids, along with triglycerides and cholesterol esters, to the apical plasma membrane in association with milk lipid secretion (pathway 1, Fig. 1) [15]. …”

Section: Introductionmentioning

confidence: 99%

Lipid Transport in the Lactating Mammary Gland

McManaman

2014

J Mammary Gland Biol Neoplasia

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Mammalian cells depend on phospholipid (PL) and fatty acid (FA) transport to maintain membrane structure and organization, and to fuel and regulate cellular functions. In mammary glands of lactating animals, copious milk secretion, including large quantities of lipid in some species, requires adaptation and integration of PL and FA synthesis and transport processes to meet secretion demands. At present few details exist about how these processes are regulated within the mammary gland. However, recent advances in our understanding of the structural and molecular biology of membrane systems and cellular lipid trafficking provide insights into the mechanisms underlying the regulation and integration of PL and FA transport processes the lactating mammary gland. This review discusses the PL and FA transport processes required to maintain the structural integrity and organization of the mammary gland and support its secretory functions within the context of current molecular and cellular models of their regulation.

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Milk lipid secretion: recent biomolecular aspects

Cited by 34 publications

References 111 publications

Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells

Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells

Multidimensional Imaging of Mammary Gland Development: A Window Into Breast Form and Function

Lipid Transport in the Lactating Mammary Gland

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