Proteome of Seed Development and Germination

Catusse, Julie; Rajjou, Loïc; Job, Claudette; Job, Dominique

doi:10.1002/9780470369630.ch13

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…This study considered seed reserve degradation and utilization for the energy needs for germination. However, some components of seed reserves may promote the transition from the quiescent to the active state during germination ( Catusse et al, 2008 ), which is certainly important and not amount dependent.…”

Section: Discussionmentioning

confidence: 99%

Mobilization and Role of Starch, Protein, and Fat Reserves during Seed Germination of Six Wild Grassland Species

et al. 2018

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Since seed reserves can influence seed germination, the quantitative and qualitative differences in seed reserves may relate to the germination characteristics of species. The purpose of our study was to evaluate the correlation between germination and seed reserves, as well as their mobilization during germination of six grassland species (Chloris virgata, Kochia scoparia, Lespedeza hedysaroides, Astragalus adsurgens, Leonurus artemisia, and Dracocephalum moldavica) and compare the results with domesticated species. We measured starch, protein, and fat content in dry seeds and the initial absorption of water during imbibition. Starch, soluble protein, fat, and soluble sugar content also were determined at five stages during germination. Starch, protein, and fat reserves in dry seeds were not significantly correlated with germination percentage and rate (speed), but soluble sugar and soluble protein contents at different germination stages were positively significantly correlated with germination rate for the six species. Starch was mainly used during seed imbibition, and soluble protein was used from the imbibition stage to the highest germination stage. Fat content for all species remained relatively constant throughout germination for six species, regardless of the proportion of other seed reserves in the seeds. Our results for fat utilization differ from those obtained for cultivated grasses and legumes. These results provide new insight on the role of seed reserves as energy resources in germination for wild species.

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Section: Discussionmentioning

confidence: 99%

Mobilization and Role of Starch, Protein, and Fat Reserves during Seed Germination of Six Wild Grassland Species

et al. 2018

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“…Considerable progress has been made in identifying and profiling proteins expressed in developing, mature, and germinating seeds [38]. In case of developing seeds, proteomic analyses have been performed in Medicago truncatula [39,40], soybean [41], and rapeseed [42,43] These large-scale proteomic studies and the past four decades of biochemical studies on oilseeds have provided a large volume of information on the metabolic pathways responsible for accumulation of storage reserves such as carbohydrates, oils, and proteins.…”

Section: Large Number Of Phosphoproteins Reside In Developing Seedmentioning

confidence: 99%

Plant phosphoproteomics: An update

et al. 2009

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Phosphoproteomics involves identification of phosphoproteins, precise mapping, and quantification of phosphorylation sites, and eventually, revealing their biological function. In plants, several systematic phosphoproteomic analyses have recently been performed to optimize in vitro and in vivo technologies to reveal components of the phosphoproteome. The discovery of novel substrates for specific protein kinases is also an important issue. Development of a new tool has enabled rapid identification of potential kinase substrates such as kinase assays using plant protein microarrays. Progress has also been made in quantitative and dynamic analysis of mapped phosphorylation sites. Increased quantity of experimentally verified phosphorylation sites in plants has prompted the creation of dedicated web-resources for plant-specific phosphoproteomics data. This resulted in development of computational prediction methods yielding significantly improved sensitivity and specificity for the detection of phosphorylation sites in plants when compared to methods trained on less plant-specific data. In this review, we present an update on phosphoproteomic studies in plants and summarize the recent progress in the computational prediction of plant phosphorylation sites. The application of the experimental and computed results in understanding the phosphoproteomic networks of cellular and metabolic processes in plants is discussed. This is a continuation of our comprehensive review series on plant phosphoproteomics.

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“…There are also a number of areas in plant biology that can be taken as excellent examples including seed biology [20,21], organelle proteomics [22], stress proteomics in plants (see Section 6), protein phosphorylation [9, and references therein], and trees [23]. Knowledge transfer between model and nonmodel plant proteomes has revealed that each plant needs to be uniquely/independently studied as many proteins and modifications are highly plant-specific.…”

Section: Information Transfer Between Model and Non-model Plants To Amentioning

confidence: 99%