Proteome microarray technology and application: higher, wider, and deeper

Qi, Huan; Wang, Fei; Tao, Sheng-Ce

doi:10.1080/14789450.2019.1662303

Cited by 21 publications

(18 citation statements)

References 70 publications

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“…Many studies have shown that HCMV infection at different stages can upregulate the levels of IL‐1α, IL‐1β, IL‐6, IL‐12, and tumor necrosis factor (TNF) ‐ α and promote inflammation and pathogenesis 20,21 . Because of its major advantages over conventional methods, such as its high‐throughput format, good sensitivity, and reduced sample consumption, 10 we used the protein chip method, followed by ELISA verification, to analyze the differential expression of CNS‐related cytokines in the serum and CSF of newborns infected with HCMV. Our results showed that Acrp30, IL‐1α, and MMP‐3 were differentially expressed in the CSF and not in the serum.…”

Section: Discussionmentioning

confidence: 99%

“…The protein chip, a new technology that has recently been developed based on the gene chip, represents a high‐throughput protein immunoassay and analysis technology. Its main advantages include miniaturization, integration, high throughput, high sensitivity, and easy operation 10 . Preliminary progress has been made in protein chips in detecting target proteins or changes in proteins related to diseases such as cancer, endocrine diseases, infectious diseases, and those of the cardiovascular system 11,12 .…”

Section: Introductionmentioning

confidence: 99%

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Neurotrophic factor levels in the serum and cerebrospinal fluid of neonates infected with human cytomegalovirus

Wang

Zou

et al. 2021

Microbiology and Immunology

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Human cytomegalovirus (HCMV) is most likely to damage the central nervous system (CNS) during early embryonic development; however, the early neurodevelopmental abnormalities caused by HCMV infection and the regulation of cytokines remain unclear. Therefore, we investigated neuronal factors in the serum and cerebrospinal fluid (CSF) of newborns infected with HCMV using protein microarray technology with a view to elucidating the changes in specific neuronal factors for use in the development of a reliable index for predicting CNS injury caused by HCMV infection. Serum and CSF were collected from four newborns with HCMV infection and CNS injury (HCMV‐infected group) and from four newborns without CNS infection (control group). A protein microarray containing 29 kinds of CNS‐related cytokines was used to identify differentially expressed neuronal factors in the serum and CSF of the HCMV‐infected and control groups. The levels of the differentially expressed proteins were verified further in 30 CSF samples from an HCMV‐infected group using enzyme‐linkedimmunosorbent assay (ELISA). Between newborns in the HCMV‐infected and control groups, the protein microarray analysis identified three differentially expressed neurotrophic factors in the CSF samples: Acrp30, MMP‐3, and interleukin‐1 alpha (IL‐1α). No differential cytokine expression was seen in the serum. ELISA showed significantly higher expression levels of Acrp30 and MMP‐3 in the CSF of the 30 newborns with HCMV infection and CNS injury than in those in the control group, whereas the expression of IL‐1α was significantly lower. Our results demonstrate that changes in the expression levels of Acrp30, MMP‐3, and IL‐1α in the CSF of newborns infected with HCMV may be related to the pathogenesis of CNS infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Neurotrophic factor levels in the serum and cerebrospinal fluid of neonates infected with human cytomegalovirus

Wang

Zou

et al. 2021

Microbiology and Immunology

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“…Although a number of review papers have been published on protein microarrays, where general illustrations of their classifications, experimental procedures, and applications could be found ( 5 , 7 , 24 , 101 , 102 ), it still seems to be rare to find a comprehensive discussion on the key issues when using protein microarrays in AID biomarker discovery. Such issues can include biosample collection, optimization of experimental design with consideration for minimized cost, choice of different protein arrays, data analysis, novel biomarker validation, and even the technical transference to clinic lab tests.…”

Section: Key Issues Of Protein Microarrays In Aid Biomarker Discoverymentioning

confidence: 99%

“…Novel label-free detection approaches such as surface plasmon resonance, carbon nanotubes, reflective phantom interface, etc. are expected to address the problem with greater sensitivity ( 101 ).…”

Section: Considerations In Design Of Protein Microarray Experimentsmentioning

confidence: 99%

Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases

Song

Bai

et al. 2021

Front. Immunol.

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Dysregulated autoantibodies and cytokines were deemed to provide important cues for potential illnesses, such as various carcinomas and autoimmune diseases. Increasing biotechnological approaches have been applied to screen and identify the specific alterations of these biomolecules as distinctive biomarkers in diseases, especially autoimmune diseases. As a versatile and robust platform, protein microarray technology allows researchers to easily profile dysregulated autoantibodies and cytokines associated with autoimmune diseases using various biological specimens, mainly serum samples. Here, we summarize the applications of protein microarrays in biomarker discovery for autoimmune diseases. In addition, the key issues in the process of using this approach are presented for improving future studies.

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“…Microarray analysis is the basis of gene function research. Differentially expressed genes can be widely, quickly and reliably screened out for functional and pathway analysis by using microarray analysis to analyze samples at different developmental stages or under different treatment conditions [8].…”

Section: Introductionmentioning

confidence: 99%

Heat Shock Cognate 70 kDa Protein Is the Target of Tetradecyl 2,3-Dihydroxybenzoate for Neuritogenic Effect in PC12 Cells

et al. 2021

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Tetradecyl 2,3-dihydroxybenzoate (ABG-001) is a lead compound derived from gentisides with a remarkable neuritogenic activity. However, the target of ABG-001 is yet to be defined to date. In this study, the potential target of ABG-001 was investigated via an activity-based protein profiling (ABPP) analysis, which is a chemical proteomic method for target identification by using chemical probes. Results indicated that the potential target proteins of ABG-001 were heat shock cognate 70 kDa protein (Hsc70), 78 kDa glucose-regulated protein (GRP78), and 14-3-3 theta protein. Then, the potential target of ABG-001 was confirmed by using inhibitors, the cellular thermal shift assay (CETSA) and small-interfering RNA (siRNA) analysis. The inhibitor of Hsc70 and siRNA significantly decreased the neurite outgrowth induced by ABG-001. Furthermore, ABG-001 induced neurite outgrowth was reduced by siRNA against Hsc70, and the results of CETSA suggested that Hsc70 showed a significant thermal stability-shifted effect upon ABG-001 treatment. These results indicated that Hsc70 is the target protein of ABG-001 in PC12 cells.

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Proteome microarray technology and application: higher, wider, and deeper

Cited by 21 publications

References 70 publications

Neurotrophic factor levels in the serum and cerebrospinal fluid of neonates infected with human cytomegalovirus

Neurotrophic factor levels in the serum and cerebrospinal fluid of neonates infected with human cytomegalovirus

Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases

Heat Shock Cognate 70 kDa Protein Is the Target of Tetradecyl 2,3-Dihydroxybenzoate for Neuritogenic Effect in PC12 Cells

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