Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation

Sangha, Jatinder Singh; Chen, Yolanda H.; Kaur, Jatinder; Khan, Wazir Zada; Abduljaleel, Zainularifeen; Alanazi, Mohammad; Mills, Aaron; Adalla, C. B.; Bennett, John; Prithiviraj, Balakrishnan; Jahn, Gary C.; Leung, Hei

doi:10.3390/ijms14023921

Cited by 33 publications

(22 citation statements)

References 56 publications

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“…Aspergillus flavus ‐resistant maize kernel embryo showed sharp up‐regulation of GLY I proteins as compared with the susceptible genotype in response to A. flavus infection (Chen et al ). Similar pattern of GLY I protein/enzyme induction was observed in rice and Brassica after brown plant hopper ( Nilaparvata lugens ) and Sclerotinia sclerotiorum infection, respectively (Chen et al ; Sangha et al ). These indicative findings point towards a potential function of glyoxalases in plant biotic stress modulation pathways.…”

Section: Introductionsupporting

confidence: 57%

Manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice

Gupta

Sahoo

Ghosh

et al. 2017

Plant Cell & Environment

108

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Crop plants face a multitude of diverse abiotic and biotic stresses in the farmers' fields. Although there now exists a considerable knowledge of the underlying mechanisms of response to individual stresses, the crosstalk between response pathways to various abiotic and biotic stresses remains enigmatic. Here, we investigated if the cytotoxic metabolite methylglyoxal (MG), excess of which is generated as a common consequence of many abiotic and biotic stresses, may serve as a key molecule linking responses to diverse stresses. For this, we generated transgenic rice plants overexpressing the entire two-step glyoxalase pathway for MG detoxification. Through assessment of various morphological, physiological and agronomic parameters, we found that glyoxalase-overexpression imparts tolerance towards abiotic stresses like salinity, drought and heat and also provides resistance towards damage caused by the sheath blight fungus (Rhizoctonia solani) toxin phenylacetic acid. We show that the mechanism of observed tolerance of the glyoxalase-overexpressing plants towards these diverse abiotic and biotic stresses involves improved MG detoxification and reduced oxidative damage leading to better protection of chloroplast and mitochondrial ultrastructure and maintained photosynthetic efficiency under stress conditions. Together, our findings indicate that MG may serve as a key link between abiotic and biotic stress response in plants.

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Section: Introductionsupporting

confidence: 57%

Manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice

Gupta

Sahoo

Ghosh

et al. 2017

Plant Cell & Environment

108

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“…Similarly, induction of GLYI activity was observed in rice in response to attack by brown planthopper (Nilaparvata lugens) and in Brassica after infection by Sclerotinia sclerotiorum. After fungal infection, intercellular MG levels were significantly increased in susceptible genotypes, so high GLYI activity was crucial for the detoxification of MG [39,40].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of glyoxalase-like gene families in grape (Vitis vinifera L.) and their expression profiling in response to downy mildew infection

Cheng

Wang

et al. 2019

BMC Genomics

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Background: The glyoxalase system usually comprises two enzymes, glyoxalase I (GLYI) and glyoxalase II (GLYII). This system converts cytotoxic methylglyoxal (MG) into non-toxic D-lactate in the presence of reduced glutathione (GSH) in two enzymatic steps. Recently, a novel type of glyoxalase III (GLYIII) activity has observed in Escherichia coli that can detoxify MG into D-lactate directly, in one step, without a cofactor. Investigation of the glyoxalase enzymes of a number of plant species shows the importance of their roles in response both to abiotic and to biotic stresses. Until now, glyoxalase gene families have been identified in the genomes of four plants, Arabidopsis, Oryza sativa, Glycine max and Medicago truncatula but no similar study has been done with the grapevine Vitis vinifera L. Results: In this study, four GLYI-like, two GLYII-like and three GLYIII-like genes are identified from the genome database of grape. All these genes were analysed in detail, including their chromosomal locations, phylogenetic relationships, exon-intron distributions, protein domain organisations and the presence of conserved binding sites. Using quantitative real-time PCR analysis (qRT-PCR), the expression profiles of these genes were analysed in different tissues of grape, and also when under infection stress from downy mildew (Plasmopara viticola). The study reveals that most VvGLY-like genes had higher expressions in stem, leaf, tendril and ovule but lower expressions in the flower. In addition, most of the VvGLY-like gene members were P. viticola responsive with high expressions 6-12 h and 96-120 h after inoculation. However, VvGLYI-like1 was highly expressed 48 h after inoculation, similar to VvPR1 and VvNPR1 which are involved in the defence response. Conclusions: This study identified the GLYI-like, GLYII-like and GLYIII-like full gene families of the grapevine. Based on a phylogenetic analysis and the presence of conserved binding sites, we speculate that these glyoxalase-like genes in grape encode active glyoxalases. Moreover, our study provides a basis for discussing the roles of VvGLYIlike, VvGLYII-like and VvGLYIII-like genes in grape's response to downy mildew infection. Our results shed light on the selection of candidate genes for downy mildew tolerance in grape and lay the foundation for further functional investigations of these glyoxalase genes.

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“…Corroborating our proteomic data, protease activity was also higher in EI rice leaves compared to control (Table 2). Proteolysis-related proteins has been shown to be induced by MeJA application on rice leaves 77 and by brown planthopper ( Nilaparvata lugens ) infestation in leaf sheaths 40 . Proteolysis processes may be associated with oxidative stress, and cells exhibit increased rates of proteolysis following exposure to oxidative stress inducing agents 78 .…”

Section: Resultsmentioning

confidence: 99%

Physiological and Molecular Alterations Promoted by Schizotetranychus oryzae Mite Infestation in Rice Leaves

Buffon¹,

Blasi²,

Adamski³

et al. 2015

J. Proteome Res.

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Infestation of phytophagous mite Schizotetranychus oryzae in rice causes critical yield losses. To better understand this interaction, we employed Multidimensional Protein Identification Technology (MudPIT) approach to identify differentially expressed proteins. We detected 18 unique proteins in control and 872 in infested leaves, respectively, along with 32 proteins more abundant in control leaves. S. oryzae infestation caused decreased abundance of proteins related to photosynthesis (mostly photosystem II-related), carbon assimilation and energy production, chloroplast detoxification, defense, fatty acid and gibberellin synthesis. On the other hand, infestation caused increased abundance of proteins involved in protein modification and degradation, gene expression at the translation level, protein partitioning to different organelles, lipid metabolism, actin cytoskeleton remodeling, and synthesis of jasmonate, amino acid and molecular chaperones. Our results also suggest that S. oryzae infestation promotes cell wall remodeling and interferes with ethylene biosynthesis in rice leaves. Proteomic data were positively correlated with enzymatic assays and RT-qPCR analysis. Our findings describe the protein expression patterns of infested rice leaves, and suggest that the acceptor side of PSII is probably the major damaged target in the photosynthetic apparatus. These data will be useful in future biotechnological approaches aiming to induce phytophagous mite resistance in rice.

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Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation

Cited by 33 publications

References 56 publications

Manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice

Manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice

Genome-wide analysis of glyoxalase-like gene families in grape (Vitis vinifera L.) and their expression profiling in response to downy mildew infection

Physiological and Molecular Alterations Promoted by Schizotetranychus oryzae Mite Infestation in Rice Leaves

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