Proteome analysis of proteins responsive to ambient and elevated ozone in rice seedlings

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

“…Ozone enters the intercellular space of leaves through stomata, and it can be converted into ROS and hydrogen peroxide, initially in the cell wall and plasma membrane (Pellinen et al, 1999), causing oxidative stress in the plant. Several physiological studies on plants have shown that ozone stress causes a decrease in the CO 2 assimilation rate (Soldatini et al, 1998), an inhibition of PS II (Guidi et al, 2001), and a reduction in RuBisCO activity in leaves (Feng et al, 2008;Pelloux et al, 2001), which ultimately affect the plant's normal growth and productivity. A comparative proteome analysis has been performed for soybean seedling subjected to stress by 120 ppb of ozone for three days.…”

Research on the Rice Proteome: The Contribution of Proteomics Technology in the Creation of Abiotic Stress-Tolerant Plants

“…Although eight of these are known salt-stress response proteins, the majority have not been reported in the literature. In proteomic studies, the level of different SOD isoforms were found up-regulated under drought [4,49], salt [1,44], and ozone [2,15]. SOD activity, determined by multigene family, is changing under different environmental conditions.…”

“…Feng et al [15] investigated the protein responses in rice seedlings under ambient conditions and under elevated ozone stress by a proteome approach. Twenty proteins were differentially expressed.…”

Impacts of Ozone (O3) and Carbon Dioxide (CO2) Environmental Pollutants on Crops: A Transcriptomics Update

“…Among all the major crops, rice (Oryza sativa L.) has been studied most for its response to O 3 -stress (Agrawal et al, 2002;Cho et al, 2008, Feng et al, 2008Frei et al, 2010). Agrawal et al (2002) first reported a detailed combined trancriptomics and proteomics response of rice plants under elevated O 3 -exposure.…”

“…Most prominent change in the rice leaves, within 24 h post-treatment with O 3 , was the induced accumulation of a pathogenesis related (PR) class 5 protein, three PR 10 class proteins, APX(s), superoxide dismutase (SOD), calcium-binding protein, calreticulin, a novel ATP-dependent CLP protease, and an unknown protein. Feng et al (2008) also followed the established two-week-old rice seedlings experimental model of Agrawal and co-workers (2002) and exposed plants to 0, 40, 80, and 120 ppb O 3 for nine days. A drastic damage in the photosynthetic proteins (mainly large and small subunits of RuBisCO) and primary metabolism related proteins was found, whereas an induced expression of some major antioxidant (i.e., glutathione-S-transferase and MnSOD) and defense/stress-related proteins (i.e., PR5 and two PR10 proteins OsPR10/PBZ1 and RSOsPR10) were reported.…”