Proteome Analysis of Poplar Seed Vigor

Abstract

Seed vigor is a complex property that determines the seed’s potential for rapid uniform emergence and subsequent growth. However, the mechanism for change in seed vigor is poorly understood. The seeds of poplar (Populus × Canadensis Moench), which are short-lived, were stored at 30°C and 75±5% relative humidity for different periods of time (0–90 days) to obtain different vigor seeds (from 95 to 0% germination). With decreasing seed vigor, the temperature range of seed germination became narrower; the respiration rate of the seeds decreased markedly, while the relative electrolyte leakage increased markedly, both levelling off after 45 days. A total of 81 protein spots showed a significant change in abundance (≥ 1.5-fold, P < 0.05) when comparing the proteomes among seeds with different vigor. Of the identified 65 proteins, most belonged to the groups involved in metabolism (23%), protein synthesis and destination (22%), energy (18%), cell defense and rescue (17%), and storage protein (15%). These proteins accounted for 95% of all the identified proteins. During seed aging, 53 and 6 identified proteins consistently increased and decreased in abundance, respectively, and they were associated with metabolism (22%), protein synthesis and destination (22%), energy (19%), cell defense and rescue (19%), storage proteins (15%), and cell growth and structure (3%). These data show that the decrease in seed vigor (aging) is an energy-dependent process, which requires protein synthesis and degradation as well as cellular defense and rescue.

Soybean Seed Vigor: Uniformity and Growth as Key Factors to Improve Yield

“…However, germination data cannot be extrapolated to the field; this is mainly because germination tests assume ideal conditions, which is contrary to the sub-optimal conditions actually present in the field [5]. Therefore, biological quality should also be measured by another concept, namely vigor, which can be more assertive in the measurement of germination rate, uniformity, and growth [6].…”

Proteomic Analysis Reveals the Positive Roles of the Plant-Growth-Promoting Rhizobacterium NSY50 in the Response of Cucumber Roots to Fusarium oxysporum f. sp. cucumerinum Inoculation

“…However, another report showed that the application of PGPR recovered the expression level of this protein under stress conditions (Li et al, 2013b). The CPN20 (spot 23), which was among the most-represented proteins that emphasize the impact of stress on post-translation modification/ modification machinery (Zhang et al, 2015) showed similar results when inoculated with FOC.…”

Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean

“…So far, many researchers have investigated seed vigor at protein level [10][11][12][13][14][15][16][17][18][19][20]. Recently, several proteomic studies have focused on the effects of HTH stress on the vigor formation of developing soybean seed.…”