Proteome analysis of Phytomonas serpens, a phytoparasite of medical interest

Júnior, Agenor de Castro Moreira dos Santos; Ricart, Carlos André Ornelas; Pontes, Arthur Henriques; Fontes, Wagner; Souza, Agnelo Rodrigues de; Castro, Mariana S.; Sousa, Marcelo Valle de; Lima, Beatriz Dolabela de

doi:10.1371/journal.pone.0204818

Cited by 4 publications

(3 citation statements)

References 55 publications

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“…The knowledge about the metabolism of P. serpens is essential to understand its pathological potential for tomato fruits, and the proteomic analysis of the parasite allowed the identification of enzymes of metabolic pathways located in glycosome, mitochondrion and cytosol ( Dos Santos Júnior et al., 2018 ). However, no enzymatic activity involved in the hydrolysis of polysaccharides into monosaccharides, such as amylases, amylomaltases, invertases or carboxymethylcellulases, were identified in P. serpens .…”

Section: Discussionmentioning

confidence: 99%

The Enhanced Expression of Cruzipain-Like Molecules in the Phytoflagellate Phytomonas serpens Recovered From the Invertebrate and Plant Hosts

Oliveira

Elias

Dias

et al. 2022

Front. Cell. Infect. Microbiol.

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Phytomonas serpens is a protozoan parasite that alternates its life cycle between two hosts: an invertebrate vector and the tomato fruit. This phytoflagellate is able to synthesize proteins displaying similarity to the cysteine peptidase named cruzipain, an important virulence factor from Trypanosoma cruzi, the etiologic agent of Chagas disease. Herein, the growth of P. serpens in complex medium (BHI) supplemented with natural tomato extract (NTE) resulted in the increased expression of cysteine peptidases, as verified by the hydrolysis of the fluorogenic substrate Z-Phe-Arg-AMC and by gelatin-SDS-PAGE. Phytoflagellates showed no changes in morphology, morphometry and viability, but the proliferation was slightly reduced when cultivated in the presence of NTE. The enhanced proteolytic activity was accompanied by a significant increase in the expression of cruzipain-like molecules, as verified by flow cytometry using anti-cruzipain antibodies. In parallel, parasites incubated under chemically defined conditions (PBS supplemented with glucose) and added of different concentration of NTE revealed an augmentation in the production of cruzipain-like molecules in a typically dose-dependent way. Similarly, P. serpens recovered from the infection of mature tomatoes showed an increase in the expression of molecules homologous to cruzipain; however, cells showed a smaller size compared to parasites grown in BHI medium. Furthermore, phytoflagellates incubated with dissected salivary glands from Oncopeltus fasciatus or recovered from the hemolymph of infected insects also showed a strong enhance in the expression of cruzipain-like molecules that is more relevant in the hemolymph. Collectively, our results showed that cysteine peptidases displaying similarities to cruzipain are more expressed during the life cycle of the phytoflagellate P. serpens both in the invertebrate and plant hosts.

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Section: Discussionmentioning

confidence: 99%

The Enhanced Expression of Cruzipain-Like Molecules in the Phytoflagellate Phytomonas serpens Recovered From the Invertebrate and Plant Hosts

Oliveira

Elias

Dias

et al. 2022

Front. Cell. Infect. Microbiol.

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“…The genomic analysis of P. serpens revealed similarities with species of the genus Leishmania and Trypanosoma , including the presence of homologue genes related to virulence such as Gp63 and cruzipain [ 5 , 6 ]. Thus, similar molecular machineries are present in both clinically relevant trypanosomatids and P. serpens , making this phytoparasite a good model for in vitro studies [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro

et al. 2023

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Phytomonas serpens is a trypanosomatid phytoparasite, found in a great variety of species, including tomato plants. It is a significant problem for agriculture, causing high economic loss. In order to reduce the vegetal infections, different strategies have been used. The biological activity of molecules obtained from natural sources has been widely investigated to treat trypanosomatids infections. Among these compounds, chalcones have been shown to have anti-parasitic and anti-inflammatory effects, being described as having a remarkable activity on trypanosomatids, especially in Leishmania species. Here, we evaluated the antiprotozoal activity of the chalcone derivative (NaF) on P. serpens promastigotes, while also assessing its mechanism of action. The results showed that treatment with the derivative NaF for 24 h promotes an important reduction in the parasite proliferation (IC50/24 h = 23.6 ± 4.6 µM). At IC50/24 h concentration, the compound induced an increase in reactive oxygen species (ROS) production and a shortening of the unique flagellum of the parasites. Electron microscopy evaluation reinforced the flagellar phenotype in treated promastigotes, and a dilated flagellar pocket was frequently observed. The treatment also promoted a prominent autophagic phenotype. An increased number of autophagosomes were detected, presenting different levels of cargo degradation, endoplasmic reticulum profiles surrounding different cellular structures, and the presence of concentric membranar structures inside the mitochondrion. Chalcone derivatives may present an opportunity to develop a treatment for the P. serpens infection, as they are easy to synthesize and are low in cost. In order to develop a new product, further studies are still necessary.

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“…An outstanding example is the existence of immunogenic similarities between Phytomonas serpens and T. cruzi . The P. serpens proteome analysis identified some proteins as candidates for potential vaccines against infections caused by kinetoplastids [10] . Therefore, the use of this parasite has been proposed as a safe experimental kinetoplastid model to develop effective chemical control agents against human and animal pathogenic trypanosomatids.…”

Section: Introductionmentioning

confidence: 99%

Biological Evaluation of Essential Oils from Selected Medicinal Plants and Their Main Components against Phytomonas davidi (Kinetoplastea: Trypanosomatidae)

Guzmán

González-Coloma⊥

Andrés

et al. 2020

Chemistry & Biodiversity

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Eight essential oils (EOs) from selected medicinal plants have been tested for their activity against Phytomonas davidi, a plant trypanosomal parasite. In the present research, the EOs have been tested on promastigote forms of P. davidi ATCC ® 30287™ strain, along with their major components, both separately and in binary combinations, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. The EOs with the highest antipromastigote activity were from Origanum virens and Salvia lavandulifolia. Thymol and β-pinene were the most active pure compounds. The study of the activity of the pure compounds in combination indicated the existence of antagonistic and synergistic effects depending on the concentration tested. In general, the combinations at low concentrations favored the activity.

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Proteome analysis of Phytomonas serpens, a phytoparasite of medical interest

Cited by 4 publications

References 55 publications

The Enhanced Expression of Cruzipain-Like Molecules in the Phytoflagellate Phytomonas serpens Recovered From the Invertebrate and Plant Hosts

The Enhanced Expression of Cruzipain-Like Molecules in the Phytoflagellate Phytomonas serpens Recovered From the Invertebrate and Plant Hosts

Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro

Biological Evaluation of Essential Oils from Selected Medicinal Plants and Their Main Components against Phytomonas davidi (Kinetoplastea: Trypanosomatidae)

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