Proteome Analysis of Cytoplasmatic and Plastidic <i>β</i>-Carotene Lipid Droplets in <i>Dunaliella bardawil</i>

Davidi, Lital; Levin, Yishai; Ben‐Dor, Shifra; Pick, Uri

doi:10.1104/pp.114.248450

Cited by 90 publications

(80 citation statements)

References 67 publications

(112 reference statements)

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“…11D). Since key biosynthetic enzymes are targeted to plastoglobules (Ytterberg et al, 2006;Schweiggert et al, 2011;Davidi et al, 2015), carotenoid biosynthesis is supposed to be initiated in these globules. Additionally, the undulating lines in Figure 11C can also been interpreted as early carotenoid depositions in tomato (Harris and Spurr, 1969).…”

Section: Discussion Two Types Of Putative Amyloplast-to-chromoplast Tmentioning

confidence: 99%

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

Zeng

Wang

et al. 2015

Plant Physiol.

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(S.X.).Globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (Citrus sinensis) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. Subsequent isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,386 putative plastid-localized proteins, 1,016 of which were quantified by spectral counting. The iTRAQ values reflecting the expression abundance of three identified proteins were validated by immunoblotting. Based on iTRAQ data, chromoplastogenesis appeared to be associated with three major protein expression patterns: (1) marked decrease in abundance of the proteins participating in the translation machinery through ribosome assembly; (2) increase in abundance of the proteins involved in terpenoid biosynthesis (including carotenoids), stress responses (redox, ascorbate, and glutathione), and development; and (3) maintenance of the proteins for signaling and DNA and RNA. Interestingly, a strong increase in abundance of several plastoglobule-localized proteins coincided with the formation of plastoglobules in the chromoplast. The proteomic data also showed that stable functioning of protein import, suppression of ribosome assembly, and accumulation of chromoplast proteases are correlated with the amyloplast-to-chromoplast transition; thus, these processes may play a collective role in chromoplast biogenesis and differentiation. By contrast, the chromoplast senescence process was inferred to be associated with significant increases in stress response and energy supply. In conclusion, this comprehensive proteomic study identified many potentially new plastid-localized proteins and provides insights into the potential developmental and molecular mechanisms underlying chromoplast biogenesis, differentiation, and senescence in sweet orange flesh.

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Section: Discussion Two Types Of Putative Amyloplast-to-chromoplast Tmentioning

confidence: 99%

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

Zeng

Wang

et al. 2015

Plant Physiol.

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“…Carotenoids are essential for photosynthesis within algal chloroplasts: they are involved in both the light harvesting and photoprotecting processes, and in stabilizing the structure of photosynthetic pigment-protein complexes and aiding in their function (Mimuro and Akimoto, 2003, Mulders et al., 2014). The halotolerant Dunaliella genus comprises green microalgae that have been intensively studied for the production of β-carotene as a valuable compound for the health food industry (Ben-Amotz et al., 1982a, Davidi et al., 2014, Tafreshi and Shariati, 2009). Dunaliella algae differ from many green algal species as their cells lack a rigid polysaccharide cell wall and are bounded only by a cytoplasmic membrane, which allows them to adjust their volume and shape rapidly in response to hypo- or hyper-osmotic changes (Sadka et al., 1989, Zelazny et al., 1995).…”

Section: Introductionmentioning

confidence: 99%

The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (chlorophyta) CCAP 19/30

Ibrahim

Harvey

2016

Plant Physiology and Biochemistry

131

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The green microalga Dunaliella salina survives in a wide range of salinities via mechanisms involving glycerol synthesis and degradation and is exploited for large amounts of nutraceutical carotenoids produced under stressed conditions. In this study, D. salina CCAP 19/30 was cultured in varying photoperiods and light intensities to study the relationship of light with different growth measurement parameters, with cellular contents of glycerol, starch and carotenoids, and with photosynthesis and respiration. Results show CCAP 19/30 regulated cell volume when growing under light/dark cycles: cell volume increased in the light and decreased in the dark, and these changes corresponded to changes in cellular glycerol content. The decrease in cell volume in the dark was independent of cell division and biological clock and was regulated by the photoperiod of the light/dark cycle. When the light intensity was increased to above 1000 μmol photons m−2 s−1, cells displayed evidence of photodamage. However, these cells also maintained the maximum level of photosynthesis efficiency and respiration possible, and the growth rate increased as light intensity increased. Significantly, the intracellular glycerol content also increased, >2-fold compared to the content in light intensity of 500 μmol photons m−2 s−1, but there was no commensurate increase in the pool size of carotenoids. These data suggest that in CCAP 19/30 glycerol stabilized the photosynthetic apparatus for maximum performance in high light intensities, a role normally attributed to carotenoids.

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“…In D . bardawil plastoglobuli several unique proteins have been identified including the major lipid-associated protein CGP [48,58]. The sequence of CGP reveals partial homology to SOUL heme-binding proteins and its relationship with the proteins of group 1.…”

Section: Discussionmentioning

confidence: 99%

Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach

Ahrazem

Argandoña

Castillo³

et al. 2016

PLoS ONE

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Using a subtractive hybridization approach, differentially expressed genes involved in the light response in saffron stigmas were identified. Twenty-two differentially expressed transcript-derived fragments were cloned and sequenced. Two of them were highly induced by light and had sequence similarity to early inducible proteins (ELIP) and SOUL heme-binding proteins. Using these sequences, we searched for other family members expressed in saffron stigma. ELIP and SOUL are represented by small gene families in saffron, with four and five members, respectively. The expression of these genes was analyzed during the development of the stigma and in light and dark conditions. ELIP transcripts were detected in all the developmental stages showing much higher expression levels in the developed stigmas of saffron and all were up-regulated by light but at different levels. By contrast, only one SOUL gene was up-regulated by light and was highly expressed in the stigma at anthesis. Both the ELIP and SOUL genes induced by light in saffron stigmas might be associated with the structural changes affecting the chromoplast of the stigma, as a result of light exposure, which promotes the development and increases the number of plastoglobules, specialized in the recruitment of specific proteins, which enables them to act in metabolite synthesis and disposal under changing environmental conditions and developmental stages.

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Proteome Analysis of Cytoplasmatic and Plastidic β-Carotene Lipid Droplets in Dunaliella bardawil

Cited by 90 publications

References 67 publications

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (chlorophyta) CCAP 19/30

Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach

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