Proteome analysis of cultivar‐specific deregulations of Oryza sativa indica and O. sativa japonica cellular suspensions undergoing Rice yellow mottle virus infection

Ventelon-Debout, Marjolaine; Delalande, François; Brizard, Jean-Paul; Diemer, Hélène; Dorsselaer, Alain Van; Brugidou, Christophe

doi:10.1002/pmic.200300502

Cited by 97 publications

(74 citation statements)

References 34 publications

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“…In plants, it was reported that HSP70 and HSP90 are essential components in the INF1-mediated hypersensitive response in Nicotiana benthamiana, and that they function upstream or independent of the MAPK cascade [23]. Moreover, various reports describe that HSP70 expression in plants is induced upon infection with pathogens [23][24][25][26], but more detailed analysis of HSP70 expression profiles during plant-pathogen interactions are scarce.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

et al. 2010

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Members of the family of 70-kD heat shock proteins (HSP70 s) play various stress-protective roles in plants. In this study, a wheat HSP70 gene was isolated from a suppression subtractive hybridization (SSH) cDNA library of wheat leaves infected by Puccinia striiformis f. sp. tritici. The gene, that was designated as TaHSC70, was predicted to encode a protein of 690 amino acids, with a molecular mass of 73.54 KDa and a pI of 5.01. Further analysis revealed the presence of a conserved signature that is characteristic for HSP70s and phylogenetic analysis demonstrated that TaHSC70 is a homolog of chloroplast HSP70s. TaHSC70 mRNA was present in leaves of both green and etiolated wheat seedlings and in stems and roots. The transcript level in roots was approximately threefold less than in leaves but light-dark treatment did not charge TaHSC70 expression. Following heat shock of wheat seedlings at 40°C, TaHSC70 expression increased in leaves of etiolated seedlings but remained stable at the same level in green seedlings. In addition, TaHSC70 was differentially expressed during an incompatible and compatible interaction with wheat-stripe rust, and there was a transient increase in expression upon treatment with methyl jasmonate (MeJA) treatment. Salicylic acid (SA), ethylene (ET) and abscisic acid (ABA) treatments had no influence on TaHSC70 expression. These results suggest that TaHSC70 plays a role in stress-related responses, and in defense responses elicited by infection with stripe rust fungus and does so via a JA-dependent signal transduction pathway.

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Section: Introductionmentioning

confidence: 99%

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

et al. 2010

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“…Although some studies have analyzed changes in global profiling resulting from virus infection of natural hosts, such as infection of cassava by African cassava mosaic virus [19] and infection of rice by rice yellow mottle virus [20], Arabidopsis thaliana has been the main model host used in combination with viruses belonging to different taxonomic families. These studies involved cauliflower mosaic caulimovirus (CaMV) [21]; turnip vein clearing (TVCV) [22•], oilseed rape mosaic (OMRV) [22•] and tobacco mosaic (TMV) tobamoviruses [23,24]; potato X potexvirus (PVX) [22•]; cucumber mosaic cucumovirus (CMV) [22•,25,26]; turnip mosaic (TuMV) [22•,27], plum pox (PPV) [28] and tobacco etch (TEV) potyviruses [29]; and mung bean yellow mosaic (MYMV) [30] and cabbage leaf curl (CaLCuV) geminiviruses [31].…”

Section: Compiling and Comparing Expression Data For Several Plant VImentioning

confidence: 99%

Detection of food-borne pathogens with DNA arrays on disk

Arnandis-Chover

Morais

Tortajada-Genaro

et al. 2012

Talanta

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ElsevierElena Fito, SF.; Carrera, J.; Rodrigo, J. (2011). A systems biology approach to the evolution of plant-virus interactions. Current Opinion in Plant Biology. 14(4):372-377. doi:10.1016/j.pbi.2011.03.013. Omic approaches to the analysis of plant-virus interactions are becoming increasingly popular. These types of data, in combination with models of interaction networks, will aid in revealing not only host components that are important for the virus life cycle, but also general patterns about the way in which different viruses manipulate host regulation of gene expression for their own benefit and possible mechanisms by which viruses evade host defenses. Here, we review studies identifying host genes regulated by viruses and discuss how these genes integrate in host regulatory and interaction networks, with a particular focus on the physical properties of these networks. A systems biology approach to the evolution of plant-virus interactions The systems biology approachGenomic tools have allowed assessment of gene expression at a genome-wide scale, providing unprecedented views of the host-virus interaction. To make use of all of the information contained in these large data sets, however, it is necessary to use computational and mathematical tools to disentangle the interactions between the molecular components of both biological entities and to identify how these interactions determine the outcome of the infection [1,2••], which is known as the field of genomic systems biology (GSB). GSB is a top-down approach that takes advantage of the recent development of high-throughput experimental techniques for obtaining omic data, and constitutes the antithesis of the reductionist paradigm (with a bottom-up perspective) that has been dominating molecular biology. The GSB approach consists of cycling between the generation of experimental data and modeling by means of reverse-engineering techniques to propose testable hypotheses about biological systems, experimental validation of these hypotheses and quantification of the relevant model parameters, and then using the newly acquired quantitative description to refine the computational model and finally make predictions of the system behavior [3,4•].To complete its infectious cycle, a few components of a virus, including its nucleic acids and encoded proteins, must establish multiple and complex interactions not only among themselves [5,6•,7,8] but also with a myriad of components of the host cell [9,10•,11]. The outcome of all these interactions is that the plant controls the spread of viral infection or, alternatively, the virus overcomes the host defenses and establishes a productive infection that may or may not be associated with the development of symptoms. While the GSB approach is being extensively used in the analysis of animal virus interactions (e.g. hepatitis C, human immunodeficiency, yellow fever, influenza A and herpesviruses), plant virologyhas not yet benefitted to the same extent, and the most relevant studies in the field generally apply some tr...

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“…2-D DiGE technology is a powerful and quantitative tool to address proteomic changes in complex samples. This and related 2-D gel techniques have been used previously to provide insight into cellular changes induced by viral infection or by specific viral proteins (1,2,7,9,14,42,53). Using this approach, we have identified 19 cellular proteins whose abundance is altered in the presence of EBNA1 in NPC cells.…”

Section: Discussionmentioning

confidence: 97%

Changes in the Nasopharyngeal Carcinoma Nuclear Proteome Induced by the EBNA1 Protein of Epstein-Barr Virus Reveal Potential Roles for EBNA1 in Metastasis and Oxidative Stress Responses

Cao

Mansouri

Frappier

2012

J Virol

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Epstein-Barr virus (EBV) infection is causatively associated with a variety of human cancers, including nasopharyngeal carcinoma (NPC).The only viral nuclear protein expressed in NPC is EBNA1, which can alter cellular properties in ways that may promote oncogenesis. Here, we used 2-dimensional difference gel electrophoresis (2-D DiGE) to profile changes in the nuclear proteome that occur after stable expression of EBNA1 in the EBV-negative NPC cell line CNE2. We found that EBNA1 consistently altered the levels of a small percentage of the nuclear proteins. The identification of 19 of these proteins by mass spectrometry revealed that EBNA1 upregulated three proteins affecting metastatic potential (stathmin 1, maspin, and Nm23-H1) and several proteins in the oxidative stress response pathway, including the antioxidants superoxide dismutase 1 (SOD1) and peroxiredoxin 1 (Prx1). Western blot analysis verified that EBNA1 expression upregulated and EBNA1 silencing downregulated these proteins. In addition, transcripts for stathmin 1 were induced by EBNA1, whereas EBNA1 only affected Prx1 and SOD1 at the protein level. Further investigation of the EBNA1 effects on the redox pathway showed that long-term EBNA1 expression in NPC resulted in increased reactive oxygen species (ROS) and increased levels of the NADPH oxidases NOX1 and NOX2, known to generate ROS. In addition, EBNA1 depletion in EBV-positive cells decreased NOX2 and ROS. The results show multiple roles for EBNA1 in the oxidative stress response pathway and suggest mechanisms by which EBNA1 may promote NPC metastases. E pstein-Barr virus (EBV) is a ubiquitous human gammaherpesvirus that is harbored in at least 90% of the world population (40). Transmitted through saliva, EBV infects B lymphocytes as well as the epithelium of the orthopharynx. Upon primary infection, EBV immediately establishes latent infection in cells by maintaining multiple copies of its genome in the form of episomes. While most infected individuals control the virus efficiently and remain free of EBV-associated diseases, latent EBV infection is linked to the development of both lymphoid and epithelial tumors. Nasopharyngeal carcinoma (NPC) is one such epithelial tumor in which EBV is widely recognized as a causative agent, since most NPC tumors are monoclonal proliferations of latently EBV-infected cells (38). These tumor cells express only one EBV nuclear protein, EBNA1, in addition to the EBV latent membrane proteins LMP1 and LMP2 (38).Epstein-Barr nuclear antigen 1 (EBNA1) is essential for the establishment of latent EBV infection since it replicates and segregates the viral genome in proliferating cells (reviewed in reference 17). Additionally, it is a transcriptional activator for other EBV latency genes. Aside from its roles in viral persistence, increasing numbers of observations suggest that EBNA1 alters cellular processes in ways that contribute to host cell survival and proliferation. First, EBNA1 is expressed in all latency forms in proliferating cells and is the only EBV protein...

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Proteome analysis of cultivar‐specific deregulations of Oryza sativa indica and O. sativa japonica cellular suspensions undergoing Rice yellow mottle virus infection

Cited by 97 publications

References 34 publications

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

Detection of food-borne pathogens with DNA arrays on disk

Changes in the Nasopharyngeal Carcinoma Nuclear Proteome Induced by the EBNA1 Protein of Epstein-Barr Virus Reveal Potential Roles for EBNA1 in Metastasis and Oxidative Stress Responses

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