Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

Abstract

Members of the family of 70-kD heat shock proteins (HSP70 s) play various stress-protective roles in plants. In this study, a wheat HSP70 gene was isolated from a suppression subtractive hybridization (SSH) cDNA library of wheat leaves infected by Puccinia striiformis f. sp. tritici. The gene, that was designated as TaHSC70, was predicted to encode a protein of 690 amino acids, with a molecular mass of 73.54 KDa and a pI of 5.01. Further analysis revealed the presence of a conserved signature that is characteristic for HSP70s and phylogenetic analysis demonstrated that TaHSC70 is a homolog of chloroplast HSP70s. TaHSC70 mRNA was present in leaves of both green and etiolated wheat seedlings and in stems and roots. The transcript level in roots was approximately threefold less than in leaves but light-dark treatment did not charge TaHSC70 expression. Following heat shock of wheat seedlings at 40°C, TaHSC70 expression increased in leaves of etiolated seedlings but remained stable at the same level in green seedlings. In addition, TaHSC70 was differentially expressed during an incompatible and compatible interaction with wheat-stripe rust, and there was a transient increase in expression upon treatment with methyl jasmonate (MeJA) treatment. Salicylic acid (SA), ethylene (ET) and abscisic acid (ABA) treatments had no influence on TaHSC70 expression. These results suggest that TaHSC70 plays a role in stress-related responses, and in defense responses elicited by infection with stripe rust fungus and does so via a JA-dependent signal transduction pathway.

Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice

“…AtHsp70s in the plastid stroma are important for thermotolerance of germinating seeds, which indicates that plastid physiology is important for seeds to endure heat stress [10]. In addition, several researchers showed that the Hsp70 family in other plant species is involved in plant development and tolerance to a variety of stresses [1, [11][12][13][14].…”

Effects Research of Environmental Hormone BPA and PAEs on the Expression of HSP70-9 Gene in Spinacia oleracea

“…Duan et al found that salicylic acid (SA), ethylene (ET) and abscisic acid (ABA) treatments had no influence on chloroplastic HSP70 expression in wheat [18]. Spinach HSP70 have sensitive response to heat [26][27][28]32], cold [28,47], water [26], wound [26] and heavy metal stress [9,12].…”

“…Although heat shock proteins (HSPs) were discovered and named after their response to thermal stress [16], HSPs are not only regulated by temperature stress, but induced by a range of other environmental stress, such as salinity, cold and heavy metals [17]. Generally it is agreed with that HSPs (especially the HSP70 and HSP90 families) play an important role in alleviating the damage caused by various environmental stresses [13,18]. HSPs can be divided into five major families: HSP100, HSP90, HSP70, HSP60 and small HSP family [19,20].…”

“…Among these families, HSP70 family is the highly conserved and the most researched gene family [11,15,17,[20][21][22][23][24]. Studies have demonstrated that heat stress [15,[18][19][20][24][25][26][27][28][29][30][31][32], Cold stress [15,24,26,29,30], water stress [22,25,26,33,34], oxidative stresses [19,20,22], heavy metals [9,12,13,35], salt stress [13,19,20,22,34], light stress [27], and chemical contaminants [11,15,18,19,25,[35]…”

Nucleotide Diversities and Genetic Relationship in the Three Japanese Pine Species; Pinus thunbergii, Pinus densiflora, and Pinus luchuensis

“…These deviations from the standard neutral model may be due to either the effect of past population structure or selection [29,54]. The Heat shock protein (HSP) and Disease resistance response protein 206 genes were suspected to be genes related to stress-related or pathogen/defense responses [55,56]. As previously stated (see Experimental Section), the currently used pine samples were initially collected from natural populations that showed special resistance characteristics to PWN.…”