Proteome analysis of bronchoalveolar lavage in lung diseases

Magi, Barbara; Bargagli, Elena; Bini, Luca; Rottoli, Paola

doi:10.1002/pmic.200600303

Cited by 104 publications

(79 citation statements)

References 130 publications

(156 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this approach suffers from large variations caused by sample preparation, protein loading, and gel staining (38). Another limit of 2DE for proteomics concerns the poor recovery of proteins from the gel for MS. Methods to supplement or replace 2DE, such as multidimensional LC have therefore been sought (39).…”

Section: Discussionmentioning

confidence: 99%

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Planque

Kulasingam

Smith

et al. 2009

Molecular & Cellular Proteomics

133

107

View full text Add to dashboard Cite

Detection of lung cancer at an early stage is necessary for successful therapy and improved survival rates. We performed a bottom-up proteomics analysis using a twodimensional LC-MS/MS strategy on the conditioned media of four lung cancer cell lines of different histological backgrounds (non-small cell lung cancer: H23 (adenocarcinoma), H520 (squamous cell carcinoma), and H460 (large cell carcinoma); small cell lung cancer: H1688) to identify secreted or membrane-bound proteins that could be useful as novel lung cancer biomarkers. Proteomics analysis of the four conditioned media allowed identification of 1,830 different proteins (965, 871, 726, and 847 from H1688, H23, H460, and H520, respectively). All proteins were assigned a subcellular localization, and 38% were classified as extracellular or membrane-bound. We successfully identified the internal control proteins (also detected by ELISA), kallikrein-related peptidases 14 and 11, and IGFBP2. We also identified known or putative lung cancer tumor markers such as squamous cell carcinoma antigen, carcinoembryonic antigen, chromogranin A, creatine kinase BB, progastrin-releasing peptide, neural cell adhesion molecule, and tumor M2-PK. To select the most promising candidates for validation, we performed tissue specificity assays, functional classifications, literature searches for association to cancer, and a comparison of our proteome with the proteome of lung-related diseases and serum. Five novel lung cancer candidates, ADAM-17, osteoprotegerin, pentraxin 3, follistatin, and tumor necrosis factor receptor superfamily member 1A were preliminarily validated in the serum of patients with lung cancer and healthy controls. Our results demonstrate the utility of this cell culture proteomics approach to identify secreted and shed proteins that are potentially useful as serologi-

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Planque

Kulasingam

Smith

et al. 2009

Molecular & Cellular Proteomics

133

107

View full text Add to dashboard Cite

show abstract

“…Several articles have been published on the proteomic composition of human BALF, especially for adults (9)(10)(11)(12). Among detected proteins that are receiving further attention, especially for their function and involvement in acute and chronic inflammatory conditions, are the α-defensins and S100A member proteins (calgranulins) (13,14).…”

mentioning

confidence: 99%

Association of high levels of α-defensins and S100A proteins with Candida mannan detection in bronchoalveolar lavage fluid of preterm neonates

et al. 2013

View full text Add to dashboard Cite

Background: Candida mannan (Mn) detection in bronchoalveolar lavage fluid (BALF) was shown to be useful for earlier identification and preemptive therapy targeting in preterm infants at high risk of invasive Candida infection. We investigated whether early detection of Candida Mn in BALF is associated with the presence of some neutrophilic products, as markers of prenatal infection/inflammation. Methods: BALF specimens were collected during the first 48 h of life from mechanically ventilated preterm newborns. Samples were analyzed by high-performance liquid chromatography-electrospray ionization-mass spectrometry. The relative amounts of α-defensins 1-4 and S100A proteins were measured by extracted ion current peak area. Absolute and differential white cell counts in BALF were obtained. Mn antigen concentrations were determined by the Platelia Candida antigen kit. results: Twenty-five studied neonates were divided into two groups: Mn-positive group and Mn-negative group. Levels of α-defensins 1-4 and S100A12 were significantly higher in the Mn-positive group than in the Mn-negative group. Moreover, positive significant correlations between the absolute number of neutrophils and the levels of α-defensins 1-4 and S100A8 were observed. conclusion: The detection of Mn antigen in BALF of preterm infants is consistent with evidence of an innate immune response in their lungs as demonstrated by higher levels of α-defensins and S100A proteins.

show abstract

“…Numerous reports have described increased oxidation with aging and have postulated a causal relationship between this increase and age-related pathologies (5,41,50,56). Increased oxidation of BAL proteins has been shown to occur in sarcoidosis, pulmonary fibrosis, and emphysema (28,34,47,56). Furthermore, using a newly improved multiplex immunoblotting approach, we showed that the increases in oxidation (carbonylation) were not random and that some proteins were preferentially oxidized.…”

Section: Discussionmentioning

confidence: 56%

Age-related changes in the expression and oxidation of bronchoalveolar lavage proteins in the rat

Umstead¹,

Freeman²,

Chinchilli³

et al. 2009

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

Umstead TM, Freeman WM, Chinchilli VM, Phelps DS. Age-related changes in the expression and oxidation of bronchoalveolar lavage proteins in the rat. Am J Physiol Lung Cell Mol Physiol 296: L14-L29, 2009. First published October 17, 2008 doi:10.1152/ajplung.90366.2008The incidence and severity of many lung diseases change with age. Some diseases, such as pneumonia, occur with increased frequency in children and the elderly. Proteins obtained by bronchoalveolar lavage (BAL) serve as the first line of defense against inhaled toxins and pathogens. Age-related changes in BAL protein expression and oxidative modification were examined in juvenile (1 mo), young adult (2 mo), and aged (18 mo) F344 rats using two-dimensional difference gel electrophoresis (2D-DIGE), matrix-assisted laser desorption ionization-time of flight/time of flight (MALDI-ToF/ToF) tandem mass spectrometry, and carbonyl immunoblotting. Using 2D-DIGE, we detected 563 protein spots, and MALDI-ToF/ToF identified 204 spots comprising 31 proteins; 21 changed significantly (17 increases) between juvenile and young adult or aged rats, but for 12 of these proteins, levels had a biphasic pattern, and levels in aged rats were less than in young adults. Relative carbonylation was determined by comparison of immunostaining with total protein staining on each oxidized protein blot. We found that aged rats had significantly increased oxidation in 13 proteins compared with juvenile rats. Many of the proteins altered in expression or oxidation level had functions in host defense, redox regulation, and protein metabolism. We speculate that low levels of expression of host defense proteins in juvenile rats and decreases in levels of these proteins between young adult and aged rats may predispose these groups to pneumonia. In addition, we have shown age-related increases in protein oxidation that may compromise host defense function in aged rats.aging; proteomics; host defense; carbonylation; proteolysis AGE IS A MAJOR FACTOR in the incidence of various lung diseases, particularly in the young and the old. Lung diseases, including chronic conditions such as emphysema and pulmonary fibrosis and acute lung diseases such as pneumonia, currently affect more than 35 million people in the U.S. Many of these diseases occur with increased frequency and severity in the elderly. However, pneumonia is also a serious problem in young children, both in developing countries, where it can be the leading cause of childhood mortality (48), and in developed countries, where morbidity is significant but mortality is low (16,43).Age also plays a role in how both humans and laboratory animals respond to pollution and various other insults. Aged organisms have an increased inflammatory response to oxidants, pollutants, and other insults (14,15,27,36,40) and are far more likely to become infected. Indeed, in the U.S., influenza and pneumonia are the fifth leading cause of death in patients over the age of 65 (37). Although it remains unclear why the elderly are more susceptible to pneumon...

show abstract

Proteome analysis of bronchoalveolar lavage in lung diseases

Cited by 104 publications

References 130 publications

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Association of high levels of α-defensins and S100A proteins with Candida mannan detection in bronchoalveolar lavage fluid of preterm neonates

Age-related changes in the expression and oxidation of bronchoalveolar lavage proteins in the rat

Contact Info

Product

Resources

About