Proteome analysis of Aspergillus niger: Lactate added in starch-containing medium can increase production of the mycotoxin fumonisin B2 by modifying acetyl-CoA metabolism

Sørensen, Louise Marie; Lametsch, René; Andersen, Mikael Rørdam; Nielsen, Per Væggemose; Frisvad, Jens Christian

doi:10.1186/1471-2180-9-255

Cited by 48 publications

(28 citation statements)

References 71 publications

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“…To compensate for this, a larger fraction of mitochondrial AcCoA must originate from pyruvate, which requires a higher flux through glycolysis. This argument is supported by the reported correlation between the glycolytic flux and the pool size of acetyl-CoA [34]. Sorensen et al (2009) observed that addition of lactate, led to an increased pool of AcCoA, which caused an up-regulation of the rate-limiting enzyme of the pentose phosphate pathway (PP pathway), Glucose-6-phosphate dehydrogenase [34].…”

Section: Discussionmentioning

confidence: 92%

Identification of a Transcription Factor Controlling pH-Dependent Organic Acid Response in Aspergillus niger

Poulsen¹,

Andersen²,

Lantz³

et al. 2012

PLoS ONE

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Acid formation in Aspergillus niger is known to be subjected to tight regulation, and the acid production profiles are fine-tuned to respond to the ambient pH. Based on transcriptome data, putative trans-acting pH responding transcription factors were listed and through knock out studies, mutants exhibiting an oxalate overproducing phenotype were identified. The yield of oxalate was increased up to 158% compared to the wild type and the corresponding transcription factor was therefore entitled Oxalic Acid repression Factor, OafA. Detailed physiological characterization of one of the ΔoafA mutants, compared to the wild type, showed that both strains produced substantial amounts of gluconic acid, but the mutant strain was more efficient in re-uptake of gluconic acid and converting it to oxalic acid, particularly at high pH (pH 5.0). Transcriptional profiles showed that 241 genes were differentially expressed due to the deletion of oafA and this supported the argument of OafA being a trans-acting transcription factor. Furthermore, expression of two phosphoketolases was down-regulated in the ΔoafA mutant, one of which has not previously been described in fungi. It was argued that the observed oxalate overproducing phenotype was a consequence of the efficient re-uptake of gluconic acid and thereby a higher flux through glycolysis. This results in a lower flux through the pentose phosphate pathway, demonstrated by the down-regulation of the phosphoketolases. Finally, the physiological data, in terms of the specific oxygen consumption, indicated a connection between the oxidative phosphorylation and oxalate production and this was further substantiated through transcription analysis.

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Section: Discussionmentioning

confidence: 92%

Identification of a Transcription Factor Controlling pH-Dependent Organic Acid Response in Aspergillus niger

Poulsen¹,

Andersen²,

Lantz³

et al. 2012

PLoS ONE

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“…For example fumonisins B 2 , B 4 and B 6 are produced by Aspergillus niger (Frisvad et al, 2007;Sørensen et al, 2009;Mogensen et al, 2009Mogensen et al, , 2010Månsson et al, 2010), while fumonisins B 1 , B 2 and B 3 are produced by a series of Fusarium species from four sections: Arthrosporiella, Dlaminia, Elegans and Liseola (Fotso et al, 2002;Rheeder et al, 2002;Sewram et al, 2005). It is not yet known whether the production of similar mycotoxins in widely different genera is caused by horizontal gene transfer (for example mobile chromosomes) or by parallel evolution.…”

Section: Mycotoxigenic Fungimentioning

confidence: 99%

Rationale for a polyphasic approach in the identification of mycotoxigenic fungi

Frisvad

2011

Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed

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“…They reduce the disulfide bonds and enhance the protein solubility. Even ampholytes at a concentration of 0.5% to 0.8% are used for increased protein solubility, however it must be taken into consideration for the focusing procedure [26]. Proteases inhibitor cocktails or PMSF are added to the homogenization buffer to deactivate the strong fungal proteases and lysis of the proteins [10].…”

Section: Sample Homogenisationmentioning

confidence: 99%

A Review beyond the borders: Proteomics of microclonial black fungi and black yeasts

Marzban¹,

Tesei²,

Sterflinger³

2013

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Black microcolonial fungi and black yeasts are inhabitants of extreme environments like vulcanic, desert and polar regions, where they are exposed to enhanced temperature alterations and desiccation. They have developed, therefore, extraordinary biologic characteristics which are mainly based on the expression of proteins, however, these are rarely studied and known. The review article presented here is focused on the obstacles and solutions for the proteomic analyses of this very particular fungal species.

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Proteome analysis of Aspergillus niger: Lactate added in starch-containing medium can increase production of the mycotoxin fumonisin B2 by modifying acetyl-CoA metabolism

Cited by 48 publications

References 71 publications

Identification of a Transcription Factor Controlling pH-Dependent Organic Acid Response in Aspergillus niger

Identification of a Transcription Factor Controlling pH-Dependent Organic Acid Response in Aspergillus niger

Rationale for a polyphasic approach in the identification of mycotoxigenic fungi

A Review beyond the borders: Proteomics of microclonial black fungi and black yeasts

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