1993
DOI: 10.1111/j.1432-1033.1993.tb18327.x
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Proteolytic cleavage of synthetic fragments of vesicle‐associated membrane protein, isoform‐2 by botulinum type B neurotoxin

Abstract: Recent data suggest that botulinum type-B neurotoxin is a protease which acts on vesicleassociated membrane protein, isoform 2 (VAMP-2). In this report, botulinum type-B neurotoxin is shown to cleave a synthetic fragment (HV62) of VAMP-2, corresponding to the bulk of the hydrophillic domain (amino acids 33-94). The neurotoxin acts at a single site between Gln76 and Phe77. Little or no proteolytic activity by botulinum type-B neurotoxin was observed with peptides containing 7, 10 or 20 amino acids spanning the … Show more

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Cited by 118 publications
(114 citation statements)
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References 28 publications
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“…SNARE proteins are thought to be the primary and perhaps only intracellular target for the BoNT͞LCs, and each serotype cleaves a different peptide bond within its corresponding SNARE substrate. This remarkable specificity results from the LCs' recognition of unusually long SNARE sequences (Ϸ30-50 residues, depending on the serotype), suggesting that substrate residues distal to the cleavage site are recognized (3)(4)(5). This hypothesis is supported by the recently elucidated crystal structure of serotype A-LC in complex with a fragment of SNAP-25 (6,7).…”
supporting
confidence: 57%
“…SNARE proteins are thought to be the primary and perhaps only intracellular target for the BoNT͞LCs, and each serotype cleaves a different peptide bond within its corresponding SNARE substrate. This remarkable specificity results from the LCs' recognition of unusually long SNARE sequences (Ϸ30-50 residues, depending on the serotype), suggesting that substrate residues distal to the cleavage site are recognized (3)(4)(5). This hypothesis is supported by the recently elucidated crystal structure of serotype A-LC in complex with a fragment of SNAP-25 (6,7).…”
supporting
confidence: 57%
“…Previous studies have shown that a VAMP(55-82) peptide which contains both the motif and cleavage site is poorly cleaved by BoNT/B [12]. This observation clearly suggests that substrate residues flanking the C-terminus of the cleavage site also play some role in substrate recognition.…”
Section: Discussionmentioning
confidence: 86%
“…Peptides were purified by reverse-phase high pressure liquid chromatography on C8 columns (2.2 × 25 cm, Vydac), and characterised as described previously [12].…”
Section: Synthesis Of Peptidesmentioning
confidence: 99%
See 1 more Smart Citation
“…Alternatively, the receptor may assist the toxin in transporting the light chain across the membrane. The final event of intoxication involves the catalytic hydrolysis of key synaptic vesicle proteins [3,[23][24][25] by the light chain [3,26]. The light chains are zinc-dependent endoproteases that selectively inactivate three essential proteins involved in the docking and fusion of acetylcholinecontaining synaptic vesicles to the plasma membrane.…”
Section: Introductionmentioning
confidence: 99%